34 results about "Cell immortalization" patented technology

The present invention provides methods for immortalizing precursor cells that are non-terminally differentiated cells such as stem cells, said methods comprising culturing the precursor cells in the presence of a Notch agonist and one or more growth factors that support the proliferation but not differentiation of the non-terminally differentiated cells. The present invention further provides methods to induce the differentiation of immortalized cells, comprising growing the cells in the presence of a Notch agonist and at least one growth factor which supports the differentiation of the cell into a more specialized cell type. The immortalized and / or differentiated cells of the invention can be used to repopulate cell populations that have been diminished, for example as a result of infection or exposure to certain drugs. The invention further provides a cell culture comprising a population of non-terminally differentiated cells immortalized by the methods of the present invention and kits comprising reagents that promote the immortalization of precursor cells. The invention further provides methods for screening for Notch modulators and for identifying genes involved in processes of cellular differentiation.

Provided are a conditional suicide anti-tumour-targeting immune cell and a preparation method therefor. The preparation method comprises the steps of: A. using a recombinant DNA technique to insert a cell-immortalized gene into a cloning site of a first cloning vector and preparing same into a virion; B. selecting specifically an immune cell in human peripheral blood by using magnetic beads, and then infecting same with the virion prepared in step A to obtain an immortalized immune cell; C. using the recombinant DNA technique to insert a suicide gene into a second cloning vector, and then transferring same into the obtained immortalized immune cell to obtain a conditional suicide immune cell; D. using the recombinant DNA technique to insert a single-chain antibody gene into a cloning site of a third cloning vector and preparing same into a virion, and then transfecting the conditional suicide immune cell to obtain a conditional suicide anti-tumour-targeting immune cell.

The invention discloses an immortalized cell line of lamb testis Sertoli cells and its establishment method and application. The lamb testis Sertoli cell immortalized cell line is named as lamb testis Sertoli cell immortalized cell line hTERT‑LSC, and its preservation number is CCTCC NO: C2018202. The experiment found that the hTERT-LSC cell line can be continuously passaged for more than 60 generations, and its growth and proliferation characteristics remain unchanged. The titer of sheeppox virus propagated by this cell line is equivalent to that of lamb testicular Sertoli cell LSC, and the cell division rate is higher than that of LSC, and the division rate can reach 1:3. At the same time, the immortalized cell line is used to prepare the vaccine, which simplifies the production process, shortens the production cycle, reduces the production cost, and ensures the stable quality of the prepared sheep pox virus vaccine. Therefore, the proposal of the present invention provides a new technical means for the large-scale production of the sheep pox virus vaccine.

The invention discloses a method for lengthening, proliferating and culturing in-vitro telomeres of cartilage cells and human tissue engineering regenerated cartilage. The method and the human tissue engineering regenerated cartilage have the advantages that telomerase mRNA [(messenger RNA (ribonucleic acid)) transfection is carried out on the cartilage cells in an in-vitro manner, accordingly, the telomeres in cartilage cells obtained by means of culturing in follow-up procedures can be lengthened, proliferation and the viability of the cartilage cells and cartilage cells of elderly patients in particular can be obviously improved by the aid of synergistic effects of the telomerase mRNA transfection and small molecule compounds such as vitamin C, B18R or p65i, rapamycin and resveratrol in complete media, and the method and the human tissue engineering regenerated cartilage have important significance in improving or treating cell function decline due to senescence when the actually applied to regeneration medical treatment; the telomeres of the cartilage cells are not continuously or even frequently lengthened by the aid of the method for lengthening, proliferating and culturing the in-vitro telomeres of the cartilage cells, accordingly, genome insertion mutation risks or cell immortalization tumor formation risks can be prevented, the safety and the applicability can be greatly improved, and the method and the human tissue engineering regenerated cartilage can be effectively applied to the regeneration medical treatment.