57 results about "Tree shrew" patented technology

The utility model discloses an experimental tree shrew breeding observation box with a noise reduction structure, which comprises a horizontally placed support base, a protective cover corresponding to the support base is arranged above the support base, an observation window is fixedly arranged on the outer surface of the front side of the protective cover, a lower baffle is fixedly arranged on the upper surface of the support base, and a noise reduction structure is arranged on the lower baffle. The lower end of the protective cover and the lower baffle are mutually clamped, a ventilation net is fixedly installed on the surface of the front side of the lower baffle, a feeder and a water feeding trough which are fixedly installed on the supporting base are arranged on the inner side of the lower baffle, and a light supplementing lamp is fixedly installed on the inner wall of the upper end of the protective cover. According to the experimental tree shrew breeding observation box with the silencing structure, a novel structural design is adopted, air is conveyed into the movable box through an air inlet pump by utilizing a connecting pipe, the air is heated by utilizing an electric heater in the movable box and then conveyed into a protective cover, and the purpose of adjusting the temperature is achieved by matching with a temperature sensor; therefore, different environment conditions can be simulated.

The application discloses a high-activity UGT1A1 enzyme based on tree shrew and a feed composition for enhancing alcohol metabolism. The catalytic efficiency of the recombinant tree shrew UGT1A1 enzyme on ethanol is significantly higher than that of human isozyme. The core of the application is to provide a complex feed system containing the enzyme, which solves the key bottleneck of poor stability and low delivery efficiency of exogenous enzymes in application through the synergistic effect of enzyme stabilizers, cell penetration enhancers, enteric coatings and coenzyme supply agents. Animal experiments show that the composition can safely and effectively accelerate in vivo alcohol elimination and reduce blood ethanol concentration, and can be used for developing alcoholism products, acute alcoholism treatment drugs and alcoholic liver disease prevention preparations.

The utility model relates to the technical field of tree shrew cages, and discloses a tree shrew cage convenient for camera shooting observation, which comprises a cage body, two side surfaces of the top end of the cage body are provided with sliding chutes I, sliding blocks I are arranged in the sliding chutes I in a sliding manner, the bottoms of the two sliding blocks I are rotatably provided with threaded rods II, and the threaded rods II are provided with threaded rods II. A second sliding block in threaded connection with the second threaded rod is slidably arranged at the top end of the interior of the cage body, an air cylinder box is arranged at the bottom end of the second sliding block, an air cylinder is arranged in the air cylinder box, the telescopic end of the air cylinder is connected with a transparent box, and a camera is arranged in the transparent box. The camera can be driven to move in the X-axis direction through the arranged first sliding block, then the camera is driven to move in the Y-axis direction through the arranged second sliding block, and finally the camera is driven to move in the Z-axis direction through the arranged air cylinder, so that the position of the camera can be changed at will, and the tree shrew can be observed and shot in all directions.

The utility model discloses a tree shrew cultivation cage capable of effectively reducing humidity, and relates to the cultivation cage field, the tree shrew cultivation cage comprises a housing and a cage rack arranged around the housing, the upper end of the housing is rotatingly provided with a cover plate, the upper end of the right side of the housing is fixedly provided with a fixed block, and the fixed block is internally provided with a quick release mechanism; the quick release mechanism is provided with a pull rod, and the pull rod penetrates through the fixing block. The tree shrew cultivation cage capable of effectively reducing humidity is provided with the cover plate which can be conveniently taken out and closed, when the tree shrew cultivation cage is used, cultivated tree shrews are placed in the shell, the tree shrews are limited by the cage frame when being placed in the shell, when the cover plate is closed, the cover plate is rotated to enable a closing block to be pressed down corresponding to the interior of a fixing block, and after a clamping block is disengaged from a clamping groove, the closing block is closed. The cover plate can be opened, so that the effect of conveniently opening and closing the breeding cage can be achieved, and the closed structure is high in strength, not prone to damage and not prone to being damaged by the tree shrew to be lost.

The utility model discloses an experimental tree shrew oral cavity opening device, which relates to the technical field of tree shrew experimental equipment and comprises a mounting plate, two connecting shafts are rotatably connected to the surface of the mounting plate, gears are arranged in the middles of the connecting shafts, the two gears are in meshed connection, a connecting rod is arranged at one end of each connecting shaft, and a connecting arm is arranged at one end of each connecting rod. One end of each connecting arm is provided with an opening assembly, and the other end of each connecting shaft is provided with a supporting rod; the tree shrew oral cavity opening device has the advantages that the holding rods enable the connecting rods to rotate outwards under the cooperation of the supporting rods and the connecting shafts, the two connecting rods rotate more synchronously through meshing connection of the two gears, the two supporting plates open the oral cavity of a tree shrew through the cooperation of the connecting rods and the connecting arms, and the tree shrew oral cavity opening device is more convenient to use. Therefore, the opening and closing degree of the two supporting plates can be adjusted through the pressure exerting force of the two holding rods, the adjusting process is convenient and efficient, and operation is safer.

The utility model discloses a temperature control device of a tree shrew breeding room, which comprises a control box with a hollow structure, a butt joint pipe is fixedly arranged on the side surface of the control box, a water adding pipe is fixedly arranged on the upper surface of the control box, and the butt joint pipe is fixedly connected with a floor heating pipeline through a joint. A conveying pump is fixedly installed outside the butt joint pipe, and the floor heating pipeline is laid under the ground in the tree shrew breeding room. According to the temperature control device of the tree shrew breeding room, novel structural design is adopted, water is filtered through a filter screen in the control box in the water flowing process, so that scale in a pipeline is reduced, in the process, a first rotating shaft and a second rotating shaft are driven to rotate through a motor and a synchronous belt, the first rotating shaft drives stirring blades to rotate, and the stirring blades are driven to rotate; and a second rotating shaft drives a cam roller to rotate, the cam roller is utilized to extrude the filter screen, and a first spring is matched to enable the filter screen to reciprocate left and right, so that the surface of the filter screen is cleaned.

The invention discloses application of a miR-5588-3p inhibitor, and belongs to the technical field of biological medicines. Researches find that the expression of miR-5588-3p in the plasma of an epileptic is abnormally increased. The miR-5588-3p inhibitor is given 72 hours before the tree shrew epilepsy model is constructed, so that the attack level, the convulsion rate and the death rate of the epilepsy tree shrew can be effectively reduced, and the brain pathological injury of the epilepsy tree shrew can be relieved. The miR-5588-3p inhibitor disclosed by the invention is used for preparing a medicine for treating epilepsy, and the nucleotide sequence of the miR-5588-3p inhibitor is as follows: GCUGGCAUUAGUGGGACUU. According to the present invention, the convulsion rate and the death rate of the epilepsy tree shrew can be well reduced, the matrix incubation period and the convulsion incubation period of the epilepsy tree shrew can be effectively prolonged, the convulsion duration of the epilepsy tree shrew can be shortened, the attack level of the epilepsy tree shrew can be effectively reduced, and the miR-5588-3p inhibitor can significantly reduce the hippocampal neuron damage.

The utility model relates to the technical field of tree shrew transportation, and discloses an improved special tree shrew transportation box which comprises a transportation box body, a first suction cup is arranged at the bottom end of the transportation box body, supporting plates are arranged on the two sides of the transportation box body, threaded rods are in threaded connection with the middles of the supporting plates, a rotating disc is arranged at the top end of each threaded rod, and a second suction cup is arranged at the bottom end of the rotating disc. A connecting plate is rotatably arranged at the bottom end of the threaded rod, a second suction cup is arranged at the bottom end of the connecting plate, positioning rods slidably connected with the supporting plate are arranged at the top end of the connecting plate and located on the two sides of the threaded rod, and limiting discs are arranged at the top ends of the positioning rods. The stability of the transport box can be improved through the arranged first suction cup, then a threaded rod is rotated and linearly moves downwards under the action of a positioning rod, so that a connecting plate and a second suction cup at the bottom end of the connecting plate are driven to move downwards, the second suction cup makes contact with the ground, the suction force between the second suction cup and the ground is increased, and therefore the stability of the transport box is improved.

The invention discloses a tree shrew Parkinson's disease model making method which comprises the following specific steps: (1) Macrozam preparation: weighing required Macrozam under a sterile condition, and dissolving the Macrozam in sterilized ultrapure water to prepare a Macrozam working solution with the concentration of 10 mg / mL; (2) gavage: gavage is conducted on the tree shrew with Macrozamine using liquid, the dosage is 40-80 mg / kg according to the mass of the tree shrew body, and a stable tree shrew Parkinson's disease model is established after normal feeding is conducted for two weeks. According to the method, the tree shrew is subjected to intragastric administration with Macrozamine, the dosage is 40-80 mg / kg, and a stable tree shrew Parkinson's disease model can be established after two weeks. According to the scheme, three transformation medical bottlenecks of a traditional model are overcome for the first time through tree shrew nigra-striatum pathway anatomy conservative property, oral transformation advantage and dosage precise control three-dimensional innovation: (1) whole course simulation; (2) channel-level fidelity; and (3) high-value prediction. The model provides an irreplaceable standardized platform for Parkinson's disease mechanism research and clinical transformation.

The utility model discloses a fence for wild cultivation of tree shrews, which relates to the technical field of tree shrew cultivation and comprises a net frame, a steel wire net is arranged in the net frame, grooves are arranged on two sides of the net frame, rotating rods are rotatably arranged in the grooves, a first clamping plate is arranged in the middle of the rotating rod on the left side, and a second clamping plate is arranged in the middle of the rotating rod on the right side. A second clamping plate matched with the first clamping plate is arranged in the middle of the rotating rod located on the right side, a limiting block is arranged in the middle of the second clamping plate, a rectangular groove is formed in the bottom of the first clamping plate, an inserting rod is slidably arranged in the rectangular groove, an inserting hole matched with the inserting rod is formed in the limiting block, a limiting assembly is arranged outside the inserting rod, and an adjusting assembly is arranged at the top of the groove. The fence has the advantages that two adjacent fences can be conveniently mounted and dismounted, an original mode of moving the fences up and down is changed into a mode of moving the fences front and back, operation difficulty is reduced, and mounting and dismounting efficiency of the fences is guaranteed.

The utility model discloses a tree shrew feeding device with an automatic feeding function, which comprises a storage barrel connected with the top of a blanking barrel made of transparent materials, the top of the storage barrel is rotatably connected with a hook, and the outer side of the hook is hung on the inner side of a breeding cage; a threaded ring is fixed to the edge of the outer side of the top of the discharging barrel, a plurality of sets of supporting strips are fixed to the edge of the outer side of the containing disc, a conical barrel is fixed to the interior of the containing disc, the outer side of the top of the conical barrel is attached to the bottom of the discharging barrel, and a plurality of sets of through holes facilitating discharging are formed in the bottom of the discharging barrel. According to the tree shrew feeding device with the automatic feeding function, the extending mechanism is arranged, by means of the extending mechanism, the distance between a containing disc and the bottom of a discharging barrel can be changed, the extending mechanism can drive a screw rod to move, a rotating disc drives a through hole to stably rotate in a discharging opening, discharging can be facilitated, and the practicability is high. And manual control of automatic feeding is avoided.

The utility model relates to the technical field of tree shrew feeding, and discloses a tree shrew feeding box convenient for separating urine and excrement, which comprises two box bodies, a channel is arranged between the two box bodies, one side of the box body close to the channel is provided with a through groove corresponding to the channel, two baffle plates are arranged in the channel in a sliding manner, and the two baffle plates are arranged in the channel in a sliding manner. A supporting plate and a collecting box are slidably arranged in the box body, the collecting box is located below the supporting plate, a plurality of liquid leakage holes are formed in the surface of the supporting plate, and a limiting seat for limiting the positions of the supporting plate and the collecting box is rotatably arranged on the side, away from the channel, of the box body through a damping rotating shaft. According to the tree shrew collecting box, separated urine and excrement can be conveniently and rapidly cleaned through the supporting plate and the collecting box which are arranged in a sliding mode, sequential dismantling is not needed, tree shrews can be transferred into the other box body during cleaning, the tree shrews do not need to be transferred to the outside any more, and operation is more convenient and faster.

The utility model discloses a water feeding device with an automatic water storage function for tree shrew cultivation, which relates to the technical field of tree shrew cultivation and comprises a kettle, the bottom end of the kettle is in threaded connection with a water outlet pipe, a limit frame is sleeved on the outer side of the kettle, and a water inlet is arranged at the top end of the kettle. An automatic water supplementing mechanism used for storing water in the kettle is arranged at the top end of the interior of the kettle and comprises a water tank, the water tank is arranged above the kettle, and a fixing rod is fixedly connected to the bottom end of the water tank. According to the water feeding device with the automatic water storage function for tree shrew cultivation, in order to guarantee sufficient water source in the kettle and utilize a water tank to independently store water, a fixed rod is inserted into a water inlet in the top end of the kettle, and a stop block is pushed downwards, so that a connecting block is driven to move downwards, and a movable plate is also pushed downwards synchronously; therefore, water in the fixing rod can automatically flow into the kettle for water supplement.

The utility model discloses a clean water supply device for tree shrew cultivation, which comprises a water storage tank internally provided with a water source, and the bottom of the water storage tank is fixedly connected with a base; a telescopic conveying pipe is connected to the interior of the water storage tank body in a penetrating mode, the top of the telescopic conveying pipe is in threaded connection with a closed cover body, and the end, stretching into the water storage tank body, of the telescopic conveying pipe is connected with a plurality of sets of spray heads; and a rotating sleeve is fixed to the bottom of the telescopic conveying pipe, a water draining mechanism is arranged at the bottom of the rotating sleeve, and the water draining mechanism acts on the bottom of the base to achieve water source discharging and cleaning treatment in the water storage tank and the base. The clean water supply device for tree shrew cultivation is provided with the water drainage mechanism, and the water drainage openings formed in the bottom of the top end of the water storage tank and the interior of the climbing frame can be opened through the arrangement of the water drainage mechanism, so that water sources in the water storage tank and the interior of the climbing frame can be replaced, and tree shrew cultivation is facilitated.

The utility model discloses an excrement collecting device for tree shrew cultivation, and relates to the field of tree shrew cultivation, the excrement collecting device comprises a base, the top of the base is fixedly provided with a supporting cylinder, the top of the supporting cylinder is fixedly connected with an excrement seat, and the outer wall of the excrement seat is sleeved with a non-slip mat; a micro motor body is arranged on the inner wall of the left side of the excrement seat, a rotating rod is fixedly mounted on an output shaft of the micro motor body through a coupler, and the tail end of the rotating rod penetrates through the supporting cylinder. The tree shrew defecation device has the advantages that the tree shrew defecation device is provided with the excrement seat and the non-slip mat, accordingly, the tree shrew can stably stand to defecate, excrement can be collected, uniformly cleaned and replaced by the aid of the collecting sleeve and the drawing box, the tree shrew can be automatically fed by the aid of the storage cylinder, the tree shrew can be attracted to defecate and collect the excrement, and the tree shrew defecation device is high in practicability. Meanwhile, an inductive sensor body and a servo motor body are arranged, and the height of the storage cylinder can be adjusted, so that the tree shrew storage device adapts to tree shrews of different heights.

The invention discloses a tree shrew visual association memory evaluation method based on an 8-shaped maze, and the method comprises the following steps: preparing before an experiment, including animal screening and environment setting; performing rule training, enabling the tree shrew to learn to associate the colors of the prompt board in the selection area with channel arm selection, including familiarity environment, door pushing training, shaping training and free selection test, and opening and closing the corresponding channel arm according to the colors of the prompt board in the shaping training; formal testing is carried out, shaping training is carried out twice every day, then free selection testing is carried out according to a 1-3 random mode, and the error rate is recorded; and performing modeling evaluation, namely evaluating whether a stable memory model is established or not based on an error rate of a formal test, and entering the formal test when the prompt board continuously appears for 1-3 times according to the same color and the conversion rate is 50-65% and the error rate is lower than 20% in the free selection test. According to the tree shrew visual association memory evaluation method provided by the invention, scientificity and statistical efficacy of behavior testing are ensured, and learning deviation caused by a fixed mode in a traditional method is overcome.

The invention discloses a tree shrew Parkinson model construction method which comprises the following steps: (1) weighing ceramide, and dissolving the ceramide in sterilized ultrapure water to prepare a solution with the concentration of 0.6 mg / mL; and (2) carrying out caudal vein injection on the tree shrew according to 4 mu g / g once per day for 7 days continuously, and normally feeding for 2 weeks after drug administration is finished to finish modeling. The stable tree shrew Parkinson's disease model can be established through caudal vein injection of ceramide with the dosage of 4 [mu] g / g for one week and normal feeding for two weeks after drug administration.

The invention discloses a tree shrew liver cancer modeling method, and belongs to the technical field of disease model construction.The method comprises the steps that 1, a reagent is prepared, under the sterile condition, needed N-nitrosodiethylamine is weighed and dissolved in sterilized ultrapure water, a 10 mg / mL N-nitrosodiethylamine using solution is prepared, and the N-nitrosodiethylamine using solution is filtered through a 0.22 micron cell filter for use; the preparation method comprises the following steps: weighing required 2-PCCA under a sterile condition, dissolving the 2-PCCA in sterilized ultrapure water to prepare a 10 mg / mL 2-PCCA working solution, and filtering the 2-PCCA working solution by using a 0.22 mu m cell filter for later use; and (2) performing intraperitoneal injection on the tree shrew by using the N-nitrosodiethylamine working solution prepared in the step (1) according to the dosage of 50mg / kg once a week, performing intragastric administration on the tree shrew by using a 2-PCCA working solution according to the dosage of 20mg / kg three days after N-nitrosodiethylamine is administered, and performing continuous 11 weeks to complete modeling. Aiming at the defects of the existing liver cancer animal model research, the invention proposes that the stable tree shrew liver cancer model can be established by jointly using DEN and 2-PCCA for the first time.

The utility model discloses a tree shrew breeding room dung tank cleaning device, which comprises a collecting tank with an uncovered structure, a first processing box and a second processing box for carrying are arranged on the left side and the right side of the collecting tank, and operating handles are fixedly arranged on the outer surfaces of the first processing box and the second processing box. A filter screen used for solid-liquid separation is fixedly mounted on the lower surface of the first treatment box, a longitudinally-arranged cleaning scraper is mounted in the collecting tank, the lower surface of the cleaning scraper is attached to the lower surface of the inner side of the collecting tank, and a flushing spray head is fixedly mounted on the left side of the cleaning scraper; and a water supply mechanism for supplying water to the flushing nozzles is arranged below the collecting tank. According to the dung tank cleaning device for the tree shrew breeding room, in the scraping and cleaning process, a water pump in the water storage tank conveys clean water into the flushing spray head through the conveying pipe, the clean water is pressurized and sprayed out through the flushing spray head, the purpose of flushing the inner wall of the collecting tank is achieved, and therefore the overall cleaning effect is improved.

The utility model discloses a convenient-to-fold foodstuff box for tree shrew cultivation, which comprises a foodstuff box body, separation cloth is arranged in the foodstuff box body, and a convenient-to-fold mechanism is arranged on the outer side of the foodstuff box body. The mechanism convenient to fold comprises a bottom plate, a connecting plate, a first threaded rod, a supporting rod, a connecting hook, a fixing buckle, a cover plate, a fixing block and a rubber cushion plate, and the bottom plate is installed below the food box body. According to the convenient-to-fold foodstuff box for tree shrew cultivation, by arranging the convenient-to-fold mechanism, the foodstuff box can be rapidly folded and placed and can also be rapidly supported and opened to be put into use, meanwhile, by arranging the fixing and mounting mechanism, the foodstuff box can be stably mounted in a cage for cultivating tree shrews, and it is guaranteed that when the tree shrews eat food, the foodstuff box can be conveniently folded and put into use. And in addition, a dismounting mechanism is arranged, so that the foodstuff box can be quickly dismounted, and the interior of the foodstuff box can be conveniently cleaned.

The utility model discloses a food mincing device for tree shrew cultivation, which comprises a mincing processing box, two groups of mincing rollers rotatably mounted in the mincing processing box, a suction hood, a motor, a motor driving device, a motor driving device, a motor driving device, a motor driving device, a motor driving device, a motor driving device, a motor driving device, a motor driving device and a motor driving device, and is characterized in that the motor is fixedly mounted at the front end of the mincing processing box and is fixedly connected with the left mincing roller; a dustproof plate is installed in the middle of the air suction cover in an inserted mode, and the dustproof plate and the air suction cover are fixed through bolts. According to the food smashing device for tree shrew cultivation, dust generated in the smashing process can be conveniently adsorbed and cleaned, dust raising is prevented, through arrangement of a screening mechanism, smashed food can be conveniently screened, the uniformity of the food is improved, left-right sliding between a screening box and a supporting block is combined, and therefore the screening efficiency is improved; and in addition, through the arrangement of a fixing plate, an auxiliary plate and an auxiliary rod, the screen mesh can be conveniently disassembled, and therefore screened large-particle impurities can be conveniently removed and cleaned.

The utility model discloses a tree shrew breeding feeder capable of quantitatively feeding, which comprises a protective shell, the protective shell is of a barrel-shaped structure for storing feeding feed, the upper end of the protective shell is sleeved with a collecting barrel, a weighing platform is arranged below the collecting barrel, and the weighing platform is arranged below the collecting barrel. And the weighing platform is used for weighing and quantifying the weight of the feed for cultivating the tree shrew fed into the feeding bowl from the protective shell. According to the feeder capable of achieving quantitative feeding for tree shrew breeding, a weighing table arranged in the protective shell is matched with a quantitative feeding assembly arranged in the collecting barrel, quantitative feeding of tree shrew grain feeding is achieved, the collecting barrel stores corresponding feed grains, natural kieselguhr is adopted for providing a dry environment to maintain the feed grains in a dry state, and the feeding efficiency is improved. The problem that too much or too little feeding occurs in traditional manual feeding is avoided, the weighing data weight threshold value of the weighing table is set through the touch screen displayer, fodder and food thrown by the quantitative throwing assembly are limited, and therefore the tree shrews at different stages can obtain sufficient fodder and food.

The invention discloses an 8-shaped labyrinth device for tree shrew cognitive decision evaluation. The 8-shaped labyrinth device comprises a central arm component, a shaped channel component and a D-shaped channel component. The central arm part comprises a central bridge and T-shaped three-way connecting parts at the two ends, and the two ends of the central bridge are connected with the ends I of the connecting parts respectively. Transverse ports of the two T-shaped three-way connecting parts are connected with the corresponding ends of the T-shaped channel part and the D-shaped channel part respectively to form a closed annular channel. The two T-shaped three-way connecting parts serve as a starting area and a selection area respectively, the starting area is provided with a side door, and one-way doors are arranged at the connecting positions of the starting area and the T-shaped channel and the D-shaped channel respectively and only allow the tree shrew to be pushed into the starting area from the T-shaped channel or the D-shaped channel in a one-way mode. The device provided by the invention structurally ensures the continuity of experiments, reduces stress caused by manual intervention, improves the accuracy and reliability of tree shrew cognitive behavior evaluation, and has the advantages of low cost, easiness in operation and convenience in cleaning and maintenance.

The utility model discloses a constant temperature nursing room for tree shrew cultivation, and relates to the technical field of tree shrew cultivation, the constant temperature nursing room comprises a heat preservation box and a box door, the front end of the heat preservation box is provided with the box door through a hinge, the inside of the heat preservation box is provided with a first breathable bedding, the left end of the first breathable bedding is connected with a second breathable bedding through a zipper, and the second breathable bedding is provided with a second breathable bedding. A material barrel is installed at the right end of the interior of the heat preservation box, a water barrel is arranged on the left side of the material barrel, a control panel is fixed to the left side face of a mounting box, a temperature sensor is fixed to the inner wall of the heat preservation box, and springs are fixed to the left side face and the right side face of the heat preservation box correspondingly. According to the constant-temperature nursing room for cultivating the tree shrews, the temperature in the heat preservation box is controlled through the temperature control structure, a comfortable living environment is provided for the tree shrews, household garbage generated by the tree shrews is conveniently cleaned, and when the tree shrews need to have a rest, a dark environment is conveniently provided for the tree shrews in the heat preservation box, and water and food are conveniently put into the tree shrews.

The utility model discloses a drinking water feeding device for tree shrew cultivation, which comprises a water feeding trough installed in a tree shrew cultivation bin, the water feeding trough and a water storage tank form a communication structure through a conveying pipe, and the middle position of the conveying pipe is connected with a conveying pump. An infrared sensor used for detecting passing of tree shrews is fixedly installed on the outer surface of the front side of the water feeding tank, an upper water tank and a lower water tank are arranged on the inner side of the interior of the water feeding tank, the upper water tank is used for water feeding operation, a partition plate is installed between the upper water tank and the lower water tank, and the partition plate is rotationally connected with the water feeding tank. According to the drinking water feeding device for tree shrew cultivation, a telescopic air cylinder contracts to pull a partition plate to rotate, water in an upper water tank and dirt enter a lower water tank, at the moment, a filter screen in the lower water tank filters the water, the filtered water is conveyed into a conveying pipe again through a backflow pipe, and the water is fed into the conveying pipe; and recycling is realized, and cleanness in the water feeding tank is kept.

The invention discloses a tree shrew work memory task training method based on an 8-shaped maze, and the method comprises the following specific operation steps: S01, preparing before task training: measuring the food intake of a tree shrew, carrying out adaptive interaction, carrying out adaptive grain limitation and setting an experimental environment; s02, a task training step: environment familiarity, shaping period and rule training are carried out in sequence, the rule training adopts a mode of shaping first and then free selection, and rewards are given only when the current arm entering selection of the tree shrew is opposite to that of the last time; and S03, delay time testing: after the tree shrews master the rules, increasing the waiting time of the starting area in a gradient manner, carrying out half-round shaping training which is the same as the testing time on the testing day, and then carrying out free selection testing. According to the tree shrew work memory task training method provided by the invention, through progressive training and personalized behavior preparation, the stress response of tree shrews is effectively reduced, the task motivation and behavior stability are improved, high accuracy can be kept within the delay time of 60 seconds, and the training efficiency is improved. And a reliable and efficient training and evaluation normal form is provided for the work memory research of the tree shrew.

The application discloses a modeling method of a tree shrew epilepsy model, and specifically comprises the following steps: under a sterile condition, 1 mg of kainic acid powder is dissolved in 0.1 ml of sterile normal saline to prepare a 10 mg / ml kainic acid solution; under the sterile condition, 1 mg of N-methyl-D-aspartate powder is dissolved in 10 ml of sterile normal saline to prepare a 100 μg / ml N-methyl-D-aspartate solution; the tree shrew is subcutaneously injected in the neck with the prepared N-methyl-D-aspartate solution at a dose of 0.4 mg / kg, 12 hours later, the tree shrew is right brain ventricle injected at a dose of 60 μg / kg, and the modeling is completed after 24 hours. According to the application, the tree shrew is subcutaneously injected in the neck with 0.4 mg / kg N-methyl-D-aspartate 12 hours before kainic acid injection, so that a low-dose kainic acid (60 μg / kg) can produce an epilepsy-causing effect on the tree shrew, the epilepsy-causing rate is 100%, and no animal dies.

The invention discloses a preparation method of a tree shrew glaucoma model, which comprises the following steps of: firstly, dissolving 25 micrograms of okadaic acid in 250 microliters of normal saline under a sterile condition to prepare a 0.1 microgram / microliter stock solution; the stock solution obtained in the step (1) is diluted with normal saline according to the proportion of 1: 5 and then injected to the tree shrew double-eye vaulted conjunctiva, the injection amount of each eye is 5 microliters on the first day and 3 microliters on the eighth day, and model manufacturing is completed after regular feeding is conducted for 21 days after administration is finished. According to the method, okadaic acid is injected to the vault conjunctiva of the two eyes of the tree shrew twice, and the specific anatomical-metabolic characteristics of the tree shrew are deeply coupled with a double-time-phase administration scheme, so that the three technical bottlenecks of'uncontrollable pathological process' (monkey), 'anatomical structure distortion '(rabbit) and'molecular mechanism deviation' (rodent) of a traditional model are successfully overcome; a first standardized animal model construction scheme with physiological correlation and operation economy is provided for glaucoma transformation medical research.

The utility model discloses a tree shrew intestinal flora collecting device, which relates to the technical field of tree shrew experimental appliances and comprises a bottom plate, a first upright post connected to one side of the top end of the bottom plate, a second upright post connected to the middle of the top end of the bottom plate, a supporting plate connected to the top ends of the first upright post and the second upright post, and a fixing mechanism arranged at the top end of the supporting plate. A through groove is formed in the middle of one side of the supporting plate, a connecting shell is connected to one side of the supporting plate, a baffle is slidably connected into the connecting shell, and a collecting mechanism is arranged on the other side of the top end of the bottom plate. The tree shrew tail limiting device has the advantages that the supporting plate is matched with the through groove to support a tree shrew, the tail of the tree shrew penetrates through the through groove to be preliminarily positioned, the connecting shell is matched with the baffle, the tail of the tree shrew can be limited between the through groove and the baffle, and therefore the tail of the tree shrew is limited and prevented from swinging, and the tree shrew tail limiting device is simple in structure and convenient to use. The excrement is prevented from being adhered to the tails of the tree shrews, so that the excrement is conveniently collected.

This invention discloses the application of a miR-5588-3p inhibitor, belonging to the field of biomedical technology. Studies have found that miR-5588-3p expression is abnormally elevated in the plasma of epilepsy patients. Administration of the miR-5588-3p inhibitor 72 hours before constructing a tree shrew epilepsy model can effectively reduce the seizure severity, seizure rate, and mortality rate in epileptic tree shrews, and alleviate brain pathological damage. The miR-5588-3p inhibitor described in this invention is used to prepare drugs for treating epilepsy, and its nucleotide sequence is as follows: GCUGGCAUUAGUGGGACUU. This invention can effectively reduce the seizure rate and mortality rate in epileptic tree shrews, effectively prolong the clonic latency and seizure latency, shorten the seizure duration, and effectively reduce the seizure severity. The miR-5588-3p inhibitor can significantly reduce hippocampal neuronal damage.