6 results about "Tree shrew" patented technology

The invention discloses application of a miR-5588-3p inhibitor, and belongs to the technical field of biological medicines. Researches find that the expression of miR-5588-3p in the plasma of an epileptic is abnormally increased. The miR-5588-3p inhibitor is given 72 hours before the tree shrew epilepsy model is constructed, so that the attack level, the convulsion rate and the death rate of the epilepsy tree shrew can be effectively reduced, and the brain pathological injury of the epilepsy tree shrew can be relieved. The miR-5588-3p inhibitor disclosed by the invention is used for preparing a medicine for treating epilepsy, and the nucleotide sequence of the miR-5588-3p inhibitor is as follows: GCUGGCAUUAGUGGGACUU. According to the present invention, the convulsion rate and the death rate of the epilepsy tree shrew can be well reduced, the matrix incubation period and the convulsion incubation period of the epilepsy tree shrew can be effectively prolonged, the convulsion duration of the epilepsy tree shrew can be shortened, the attack level of the epilepsy tree shrew can be effectively reduced, and the miR-5588-3p inhibitor can significantly reduce the hippocampal neuron damage.

The invention discloses a tree shrew liver cancer modeling method, and belongs to the technical field of disease model construction.The method comprises the steps that 1, a reagent is prepared, under the sterile condition, needed N-nitrosodiethylamine is weighed and dissolved in sterilized ultrapure water, a 10 mg / mL N-nitrosodiethylamine using solution is prepared, and the N-nitrosodiethylamine using solution is filtered through a 0.22 micron cell filter for use; the preparation method comprises the following steps: weighing required 2-PCCA under a sterile condition, dissolving the 2-PCCA in sterilized ultrapure water to prepare a 10 mg / mL 2-PCCA working solution, and filtering the 2-PCCA working solution by using a 0.22 mu m cell filter for later use; and (2) performing intraperitoneal injection on the tree shrew by using the N-nitrosodiethylamine working solution prepared in the step (1) according to the dosage of 50mg / kg once a week, performing intragastric administration on the tree shrew by using a 2-PCCA working solution according to the dosage of 20mg / kg three days after N-nitrosodiethylamine is administered, and performing continuous 11 weeks to complete modeling. Aiming at the defects of the existing liver cancer animal model research, the invention proposes that the stable tree shrew liver cancer model can be established by jointly using DEN and 2-PCCA for the first time.

This invention discloses the application of a miR-5588-3p inhibitor, belonging to the field of biomedical technology. Studies have found that miR-5588-3p expression is abnormally elevated in the plasma of epilepsy patients. Administration of the miR-5588-3p inhibitor 72 hours before constructing a tree shrew epilepsy model can effectively reduce the seizure severity, seizure rate, and mortality rate in epileptic tree shrews, and alleviate brain pathological damage. The miR-5588-3p inhibitor described in this invention is used to prepare drugs for treating epilepsy, and its nucleotide sequence is as follows: GCUGGCAUUAGUGGGACUU. This invention can effectively reduce the seizure rate and mortality rate in epileptic tree shrews, effectively prolong the clonic latency and seizure latency, shorten the seizure duration, and effectively reduce the seizure severity. The miR-5588-3p inhibitor can significantly reduce hippocampal neuronal damage.

The invention discloses sgRNA for accurately editing a PSEN1 p.M146V site of a tree shrew and application of the sgRNA, and belongs to the technical field of gene editing. The nucleotide sequence of the sgRNA designed by the invention is shown as SEQ ID NO.1. The sgRNA can guide a base editor to be precisely positioned to a PSEN1 p.M146V site, a spermatogonial stem cell line for precisely editing the PSEN1 p.M146V site of the tree shrew is obtained, the precisely edited spermatogonial stem cell line is obtained through single cell cloning, screening and enrichment, and the stably inherited PSEN1 p.M146V gene is obtained through reproductive line transmission to edit the tree shrew. The sgRNA provided by the invention is accurate in design and high in editing efficiency, and the editing efficiency of the obtained tree shrew spermatogonial stem cells is as high as 100%. The spermatogonial stem cell line for precisely editing the tree shrew PSEN1 p.M146V site and the stably inherited PSEN1 p.M146V gene editing tree shrew are obtained for the first time, a research basis is provided for researching the degenerative phenotype, pathological characteristics and molecular mechanism of the Alzheimer's disease caused by the mutation, and an important experimental platform is provided for screening related drugs.

The utility model relates to the technical field of animal behavior experiment equipment, and provides a water maze platform suitable for tree shrews, which comprises a transparent acrylic material platform, a waterproof shell, a lifting device and an intelligent detection control system. The platform adapts to the body type of the tree shrew; an attitude sensor is arranged at the bottom of the platform and communicates with a control circuit board through a waterproof connecting line, the load change of the platform is detected in real time, the lifting device is controlled to slowly jack the platform for a period of time and then automatically reset, and lifting time data are synchronously transmitted to a terminal through a wireless transmission module. The lifting device adopts a screw rod to drive a sleeve to drive the platform to lift, and the waterproof effect of the device is ensured by combining an oil seal in a waterproof shell and a thread sealing design; #-shaped grooves or raised lines are arranged on the surface of the platform so as to enhance friction force and regional identifiability. Through the platform lifting triggered by animal behaviors, the problems that a traditional water maze platform is poor in adaptability to tree shrews and lacks intelligent interaction are solved, and the experiment efficiency and precision are remarkably improved.

This invention discloses a method for establishing Alzheimer's disease in tree shrews. The specific steps involve mixing an ethanol solution of 0.5 μg / μl Microcystin-LR with a muscarine solution, and then injecting 2 μl into each of the bilateral Meynert basal ganglia of the tree shrew. After administration, the tree shrew is fed normally for two weeks to complete the Alzheimer's disease model. The muscarine solution is prepared under sterile conditions by adding 200 μl of sterile saline to a vial containing 1 mg of muscarine, repeatedly pipetting to ensure complete dissolution, resulting in a concentration of 5 μg / μl. The Microcystin-LR solution is a commercially available product. This invention involves injecting a mixture of 0.1 μg MC-LR and 3 μg IBO solution into the bilateral Meynert basal ganglia of tree shrews, establishing a stable tree shrew AD model after two weeks. Three days after administration, tree shrews exhibited AD behavioral manifestations, such as lethargy and reduced activity. Two weeks later, the tree shrew brains showed typical AD pathological manifestations, including Aβ deposition and elevated expression levels at multiple phosphorylation sites of Tau protein.