19 results about "Tree shrew" patented technology

The utility model discloses an experimental tree shrew breeding observation box with a noise reduction structure, which comprises a horizontally placed support base, a protective cover corresponding to the support base is arranged above the support base, an observation window is fixedly arranged on the outer surface of the front side of the protective cover, a lower baffle is fixedly arranged on the upper surface of the support base, and a noise reduction structure is arranged on the lower baffle. The lower end of the protective cover and the lower baffle are mutually clamped, a ventilation net is fixedly installed on the surface of the front side of the lower baffle, a feeder and a water feeding trough which are fixedly installed on the supporting base are arranged on the inner side of the lower baffle, and a light supplementing lamp is fixedly installed on the inner wall of the upper end of the protective cover. According to the experimental tree shrew breeding observation box with the silencing structure, a novel structural design is adopted, air is conveyed into the movable box through an air inlet pump by utilizing a connecting pipe, the air is heated by utilizing an electric heater in the movable box and then conveyed into a protective cover, and the purpose of adjusting the temperature is achieved by matching with a temperature sensor; therefore, different environment conditions can be simulated.

The application discloses a high-activity UGT1A1 enzyme based on tree shrew and a feed composition for enhancing alcohol metabolism. The catalytic efficiency of the recombinant tree shrew UGT1A1 enzyme on ethanol is significantly higher than that of human isozyme. The core of the application is to provide a complex feed system containing the enzyme, which solves the key bottleneck of poor stability and low delivery efficiency of exogenous enzymes in application through the synergistic effect of enzyme stabilizers, cell penetration enhancers, enteric coatings and coenzyme supply agents. Animal experiments show that the composition can safely and effectively accelerate in vivo alcohol elimination and reduce blood ethanol concentration, and can be used for developing alcoholism products, acute alcoholism treatment drugs and alcoholic liver disease prevention preparations.

The utility model discloses a tree shrew cultivation cage capable of effectively reducing humidity, and relates to the cultivation cage field, the tree shrew cultivation cage comprises a housing and a cage rack arranged around the housing, the upper end of the housing is rotatingly provided with a cover plate, the upper end of the right side of the housing is fixedly provided with a fixed block, and the fixed block is internally provided with a quick release mechanism; the quick release mechanism is provided with a pull rod, and the pull rod penetrates through the fixing block. The tree shrew cultivation cage capable of effectively reducing humidity is provided with the cover plate which can be conveniently taken out and closed, when the tree shrew cultivation cage is used, cultivated tree shrews are placed in the shell, the tree shrews are limited by the cage frame when being placed in the shell, when the cover plate is closed, the cover plate is rotated to enable a closing block to be pressed down corresponding to the interior of a fixing block, and after a clamping block is disengaged from a clamping groove, the closing block is closed. The cover plate can be opened, so that the effect of conveniently opening and closing the breeding cage can be achieved, and the closed structure is high in strength, not prone to damage and not prone to being damaged by the tree shrew to be lost.

The invention discloses application of a miR-5588-3p inhibitor, and belongs to the technical field of biological medicines. Researches find that the expression of miR-5588-3p in the plasma of an epileptic is abnormally increased. The miR-5588-3p inhibitor is given 72 hours before the tree shrew epilepsy model is constructed, so that the attack level, the convulsion rate and the death rate of the epilepsy tree shrew can be effectively reduced, and the brain pathological injury of the epilepsy tree shrew can be relieved. The miR-5588-3p inhibitor disclosed by the invention is used for preparing a medicine for treating epilepsy, and the nucleotide sequence of the miR-5588-3p inhibitor is as follows: GCUGGCAUUAGUGGGACUU. According to the present invention, the convulsion rate and the death rate of the epilepsy tree shrew can be well reduced, the matrix incubation period and the convulsion incubation period of the epilepsy tree shrew can be effectively prolonged, the convulsion duration of the epilepsy tree shrew can be shortened, the attack level of the epilepsy tree shrew can be effectively reduced, and the miR-5588-3p inhibitor can significantly reduce the hippocampal neuron damage.

The utility model discloses a tree shrew feeding device with an automatic feeding function, which comprises a storage barrel connected with the top of a blanking barrel made of transparent materials, the top of the storage barrel is rotatably connected with a hook, and the outer side of the hook is hung on the inner side of a breeding cage; a threaded ring is fixed to the edge of the outer side of the top of the discharging barrel, a plurality of sets of supporting strips are fixed to the edge of the outer side of the containing disc, a conical barrel is fixed to the interior of the containing disc, the outer side of the top of the conical barrel is attached to the bottom of the discharging barrel, and a plurality of sets of through holes facilitating discharging are formed in the bottom of the discharging barrel. According to the tree shrew feeding device with the automatic feeding function, the extending mechanism is arranged, by means of the extending mechanism, the distance between a containing disc and the bottom of a discharging barrel can be changed, the extending mechanism can drive a screw rod to move, a rotating disc drives a through hole to stably rotate in a discharging opening, discharging can be facilitated, and the practicability is high. And manual control of automatic feeding is avoided.

The invention discloses a tree shrew visual association memory evaluation method based on an 8-shaped maze, and the method comprises the following steps: preparing before an experiment, including animal screening and environment setting; performing rule training, enabling the tree shrew to learn to associate the colors of the prompt board in the selection area with channel arm selection, including familiarity environment, door pushing training, shaping training and free selection test, and opening and closing the corresponding channel arm according to the colors of the prompt board in the shaping training; formal testing is carried out, shaping training is carried out twice every day, then free selection testing is carried out according to a 1-3 random mode, and the error rate is recorded; and performing modeling evaluation, namely evaluating whether a stable memory model is established or not based on an error rate of a formal test, and entering the formal test when the prompt board continuously appears for 1-3 times according to the same color and the conversion rate is 50-65% and the error rate is lower than 20% in the free selection test. According to the tree shrew visual association memory evaluation method provided by the invention, scientificity and statistical efficacy of behavior testing are ensured, and learning deviation caused by a fixed mode in a traditional method is overcome.

The invention discloses a tree shrew liver cancer modeling method, and belongs to the technical field of disease model construction.The method comprises the steps that 1, a reagent is prepared, under the sterile condition, needed N-nitrosodiethylamine is weighed and dissolved in sterilized ultrapure water, a 10 mg / mL N-nitrosodiethylamine using solution is prepared, and the N-nitrosodiethylamine using solution is filtered through a 0.22 micron cell filter for use; the preparation method comprises the following steps: weighing required 2-PCCA under a sterile condition, dissolving the 2-PCCA in sterilized ultrapure water to prepare a 10 mg / mL 2-PCCA working solution, and filtering the 2-PCCA working solution by using a 0.22 mu m cell filter for later use; and (2) performing intraperitoneal injection on the tree shrew by using the N-nitrosodiethylamine working solution prepared in the step (1) according to the dosage of 50mg / kg once a week, performing intragastric administration on the tree shrew by using a 2-PCCA working solution according to the dosage of 20mg / kg three days after N-nitrosodiethylamine is administered, and performing continuous 11 weeks to complete modeling. Aiming at the defects of the existing liver cancer animal model research, the invention proposes that the stable tree shrew liver cancer model can be established by jointly using DEN and 2-PCCA for the first time.

The invention discloses an 8-shaped labyrinth device for tree shrew cognitive decision evaluation. The 8-shaped labyrinth device comprises a central arm component, a shaped channel component and a D-shaped channel component. The central arm part comprises a central bridge and T-shaped three-way connecting parts at the two ends, and the two ends of the central bridge are connected with the ends I of the connecting parts respectively. Transverse ports of the two T-shaped three-way connecting parts are connected with the corresponding ends of the T-shaped channel part and the D-shaped channel part respectively to form a closed annular channel. The two T-shaped three-way connecting parts serve as a starting area and a selection area respectively, the starting area is provided with a side door, and one-way doors are arranged at the connecting positions of the starting area and the T-shaped channel and the D-shaped channel respectively and only allow the tree shrew to be pushed into the starting area from the T-shaped channel or the D-shaped channel in a one-way mode. The device provided by the invention structurally ensures the continuity of experiments, reduces stress caused by manual intervention, improves the accuracy and reliability of tree shrew cognitive behavior evaluation, and has the advantages of low cost, easiness in operation and convenience in cleaning and maintenance.

The invention discloses a tree shrew work memory task training method based on an 8-shaped maze, and the method comprises the following specific operation steps: S01, preparing before task training: measuring the food intake of a tree shrew, carrying out adaptive interaction, carrying out adaptive grain limitation and setting an experimental environment; s02, a task training step: environment familiarity, shaping period and rule training are carried out in sequence, the rule training adopts a mode of shaping first and then free selection, and rewards are given only when the current arm entering selection of the tree shrew is opposite to that of the last time; and S03, delay time testing: after the tree shrews master the rules, increasing the waiting time of the starting area in a gradient manner, carrying out half-round shaping training which is the same as the testing time on the testing day, and then carrying out free selection testing. According to the tree shrew work memory task training method provided by the invention, through progressive training and personalized behavior preparation, the stress response of tree shrews is effectively reduced, the task motivation and behavior stability are improved, high accuracy can be kept within the delay time of 60 seconds, and the training efficiency is improved. And a reliable and efficient training and evaluation normal form is provided for the work memory research of the tree shrew.

The application discloses a modeling method of a tree shrew epilepsy model, and specifically comprises the following steps: under a sterile condition, 1 mg of kainic acid powder is dissolved in 0.1 ml of sterile normal saline to prepare a 10 mg / ml kainic acid solution; under the sterile condition, 1 mg of N-methyl-D-aspartate powder is dissolved in 10 ml of sterile normal saline to prepare a 100 μg / ml N-methyl-D-aspartate solution; the tree shrew is subcutaneously injected in the neck with the prepared N-methyl-D-aspartate solution at a dose of 0.4 mg / kg, 12 hours later, the tree shrew is right brain ventricle injected at a dose of 60 μg / kg, and the modeling is completed after 24 hours. According to the application, the tree shrew is subcutaneously injected in the neck with 0.4 mg / kg N-methyl-D-aspartate 12 hours before kainic acid injection, so that a low-dose kainic acid (60 μg / kg) can produce an epilepsy-causing effect on the tree shrew, the epilepsy-causing rate is 100%, and no animal dies.

This invention discloses the application of a miR-5588-3p inhibitor, belonging to the field of biomedical technology. Studies have found that miR-5588-3p expression is abnormally elevated in the plasma of epilepsy patients. Administration of the miR-5588-3p inhibitor 72 hours before constructing a tree shrew epilepsy model can effectively reduce the seizure severity, seizure rate, and mortality rate in epileptic tree shrews, and alleviate brain pathological damage. The miR-5588-3p inhibitor described in this invention is used to prepare drugs for treating epilepsy, and its nucleotide sequence is as follows: GCUGGCAUUAGUGGGACUU. This invention can effectively reduce the seizure rate and mortality rate in epileptic tree shrews, effectively prolong the clonic latency and seizure latency, shorten the seizure duration, and effectively reduce the seizure severity. The miR-5588-3p inhibitor can significantly reduce hippocampal neuronal damage.

The utility model discloses an experimental tree shrew rearing cage convenient to clean and disinfect excrement, and relates to the technical field of tree shrew rearing, the experimental tree shrew rearing cage comprises an outer frame, a rearing cage body is installed in the outer frame, a cross rod is fixedly connected to the middle of the top of the outer frame, and the cross rod forms a handle at the top of the outer frame. Symmetrically-distributed guide rails are fixedly connected to the two sides of the bottom of the rearing cage body, the two guide rails are fixedly connected to the inner side of the outer frame, a collecting drawer is fixedly connected between the two guide rails, and the top of the collecting drawer is aligned to the bottom of the rearing cage body. According to the experimental tree shrew rearing cage facilitating excrement cleaning and disinfection, due to the arranged film structure, excrement directly falls onto a film in the rearing process, direct contact between the excrement and a collecting drawer or the rearing cage body is avoided, and the automatic scraping function of a fixing rod and a cleaning sleeve is combined; excrement attached to the metal rod is automatically cleaned while the collecting drawer is pulled out, so that the overall sanitary condition of the equipment is improved.

The invention discloses sgRNA for accurately editing a PSEN1 p.M146V site of a tree shrew and application of the sgRNA, and belongs to the technical field of gene editing. The nucleotide sequence of the sgRNA designed by the invention is shown as SEQ ID NO.1. The sgRNA can guide a base editor to be precisely positioned to a PSEN1 p.M146V site, a spermatogonial stem cell line for precisely editing the PSEN1 p.M146V site of the tree shrew is obtained, the precisely edited spermatogonial stem cell line is obtained through single cell cloning, screening and enrichment, and the stably inherited PSEN1 p.M146V gene is obtained through reproductive line transmission to edit the tree shrew. The sgRNA provided by the invention is accurate in design and high in editing efficiency, and the editing efficiency of the obtained tree shrew spermatogonial stem cells is as high as 100%. The spermatogonial stem cell line for precisely editing the tree shrew PSEN1 p.M146V site and the stably inherited PSEN1 p.M146V gene editing tree shrew are obtained for the first time, a research basis is provided for researching the degenerative phenotype, pathological characteristics and molecular mechanism of the Alzheimer's disease caused by the mutation, and an important experimental platform is provided for screening related drugs.

The utility model discloses a tree shrew cub incubator with a temperature control monitoring structure, and relates to the field of tree shrew cultivation, the tree shrew cub incubator comprises a mounting shell, the side surface of the mounting shell is provided with a central console, the side surface of the mounting shell is provided with a driving motor, the outer surface of the output end of the driving motor is provided with a first fixed gear, and the first fixed gear is provided with a temperature control monitoring structure. And a meshing sliding mechanism for moving the cleaning sliding plate is mounted above the first fixed gear and comprises a supporting moving net. According to the tree shrew cub incubator with the temperature control monitoring structure, when the interior of the device needs to be cooled, a fixed abutting rod is synchronously driven when a driving motor drives a meshing rack to slide, the fixed abutting rod makes contact with a transmission line set in the moving process, and meanwhile a sliding transmission rack at the tail end is driven to slide; and in the sliding process of the sliding transmission rack, the fixed cooling fan is driven to rotate through the second fixed gear, and the cooling effect on the interior of the equipment is achieved.

The application discloses a special eight-arm maze device for testing the spatial learning and memory ability of tree shrews, which comprises a central platform device, a starting arm device, an experimental arm device and a support plate, and the eight-arm maze device is composed of the central platform device, seven experimental arm devices and one starting arm device which are uniformly arranged in a Chinese character'mi' type around the central platform device, and the experimental arm device and the starting arm device are fixedly connected with the central platform device by screws on both sides of the support plate. In addition, the central platform and the seven experimental arms are designed by splicing a semitransparent material, the visual field of the tree shrews in the maze is expanded, the animals are facilitated to position according to the space reference outside the maze, the tree shrews are more likely to receive and refer to environmental information, and efficient learning of the tree shrews is facilitated; the side plate of the starting arm is designed to be opaque, the interference of environmental and artificial factors on the tree shrews during the rest period of the tree shrews in the starting arm after the experiment is reduced, and the safety of the tree shrews is improved.

1. Name of the designed product: wool ball (banded deer). 2. Use of the designed product: the designed product is used for drying in a dryer. 3. Design points of the designed product: combination of shape and pattern. 4. Picture or photo best indicating the design points: front view.

The utility model relates to the technical field of animal behavior experiment equipment, and provides a water maze platform suitable for tree shrews, which comprises a transparent acrylic material platform, a waterproof shell, a lifting device and an intelligent detection control system. The platform adapts to the body type of the tree shrew; an attitude sensor is arranged at the bottom of the platform and communicates with a control circuit board through a waterproof connecting line, the load change of the platform is detected in real time, the lifting device is controlled to slowly jack the platform for a period of time and then automatically reset, and lifting time data are synchronously transmitted to a terminal through a wireless transmission module. The lifting device adopts a screw rod to drive a sleeve to drive the platform to lift, and the waterproof effect of the device is ensured by combining an oil seal in a waterproof shell and a thread sealing design; #-shaped grooves or raised lines are arranged on the surface of the platform so as to enhance friction force and regional identifiability. Through the platform lifting triggered by animal behaviors, the problems that a traditional water maze platform is poor in adaptability to tree shrews and lacks intelligent interaction are solved, and the experiment efficiency and precision are remarkably improved.

This invention discloses a method for establishing Alzheimer's disease in tree shrews. The specific steps involve mixing an ethanol solution of 0.5 μg / μl Microcystin-LR with a muscarine solution, and then injecting 2 μl into each of the bilateral Meynert basal ganglia of the tree shrew. After administration, the tree shrew is fed normally for two weeks to complete the Alzheimer's disease model. The muscarine solution is prepared under sterile conditions by adding 200 μl of sterile saline to a vial containing 1 mg of muscarine, repeatedly pipetting to ensure complete dissolution, resulting in a concentration of 5 μg / μl. The Microcystin-LR solution is a commercially available product. This invention involves injecting a mixture of 0.1 μg MC-LR and 3 μg IBO solution into the bilateral Meynert basal ganglia of tree shrews, establishing a stable tree shrew AD model after two weeks. Three days after administration, tree shrews exhibited AD behavioral manifestations, such as lethargy and reduced activity. Two weeks later, the tree shrew brains showed typical AD pathological manifestations, including Aβ deposition and elevated expression levels at multiple phosphorylation sites of Tau protein.

The utility model relates to the technical field of animal feeding, in particular to a tree shrew living body feed feeding device which comprises a main shell and a feed bin, the feed bin is formed in the main shell and used for storing feed, a supporting seat is arranged on the outer side of the main shell, a movable seat is arranged above the supporting seat, a reset spring is connected to the lower portion of the movable seat, and the reset spring is connected with a feed bin. A movable seat is arranged in the main shell, upward thrust is provided for the movable seat through a reset spring, a feeding groove is formed in the inner side of the movable seat and formed in the side edge of the main shell, a movable roller is arranged above the feeding groove, a material containing groove is formed above the movable roller, and a rotation control mechanism is arranged on the outer side of the movable roller. According to the tree shrew living body feed feeding device, feed in the feed bin can be brought out into the feeding trough through rotation of the movable roller in cooperation with the feed containing trough so that feeding can be achieved, the movable roller can reset after feeding, and the feed containing trough can be moved into the feed bin again to make preparations for next-time feeding.