45 results about "Response reactions" patented technology

The present invention combines synthetic, natural and recombinant polymers for making a self-retaining suture. In an embodiment of the present invention recombinant polyhydroxyalkanoate (rPHA) polymers and / or polyglycolic acid polymers are incorporated into the suture. In various embodiments of the invention, materials are incorporated into the suture to illicit a tissue response reaction. The tissue response reaction results in deposition of collagen around the tissue retainer in the tissue thereby increasing the retention of the tissue retainer in the tissue. In various embodiments of the invention, the core of the suture is made from a material that does not illicit a tissue response reaction. In various embodiments of the invention, the core can be chose to enhance elasticity and strength of the suture.

The invention provides recombination interferon (rSIFN-co) or the functional analog with new space conformation, enhanced effect, and low side effect. It is showed by the external pharmacodynamics, it can not only restrain the DNA replication of the hepatitis b virus, but also restrain the exudation of the surface antigen (HBsAg) and e antigen (HBeAg). The cytology toxicity is 1 / 8 of the clinical interferon and the antiviral activity is 5-20 times to the clinical interferon and has a higher, a more board spectrum and longer time biological response reaction, and prevent the tumor hyperplasia and transmission. The invention also provides the synthetic gene code, gene carrier, expression system of the synthetic gene of the recombination interferon or the functional analog. Finally, the invention also provides the preparing method and application thereof.

The invention discloses a preparation method of a periodic wuchereria malayi compound multivalent DNA (deoxyribonucleic acid) vaccine. The preparation method comprises the following steps of: designing a primer, amplifying BmGAPDH (reduced glyceraldehyde-phosphate dehydrogenase) and BmCPI genes by PCR (polymerase chain reaction), purifying segments of BmGAPDH and BmCPI genes, connecting and converting a T carrier, screening and identifying an I positive recombinant clone, building and identifying an I recombinant expression vector, extracting and purifying a mass of recombinant plasmid and the like. The preparation method is convenient in preparation and good in effect. The periodic wuchereria malayi which is popular in China is taken as a research object, and the periodic wuchereria malayi cysteine proteinase inhibitor / 3-phosphoglyceraldehyde dehydrogenase eukaryotic expression recombinant plasmid is built. The test result proves that the compound multivalent DNA vaccine can induce the mouse to generate the specific immune response reaction, so that a satisfied effect can be obtained, and the periodic wuchereria malayi compound multivalent DNA vaccine can be successfully prepared.

The invention provides a preparation method and application of a new therapeutic vaccine for dendritic tumor cells. The therapeutic tumor vaccine is chemotherapeutic drug induced tumor antigen, wherein, the dendritic cells are loaded and activated. The chemotherapeutic medicament induced tumor antigen contains a plurality of immunostimulation molecules as well as tumor-associated and specific antigen, which can remarkably carry out chemotaxis and activation on immunocytes such as the dendritic cells, T-cells (thymus-dependent lymphocytes) and the like, stimulate the dendritic cells to be mature and express a plurality of cell factors and chemotactic factors, effectively induce immune response reaction with antigenic specificity and non-specificity and strengthen body immune function. The therapeutic vaccine provided by the invention can be used for preventing and treating tumors and has the characteristics of simple preparation process, low cost, strong specificity, obvious curative effect and the like.

The invention relates to an inactivated type lactic acid bacteria microecological preparation as well as a preparation method and application thereof. The inactivated type lactic acid bacteria microecological preparation comprises inactivated bacteria and auxiliary materials, wherein the inactivated bacteria comprise inactivated bifidobacteria and inactivated bacterium lacticum. The inactivated microecological preparation disclosed by the invention is high in safety, resistant to infection by bacteriophages, free from limit of bacterium quantity, and high in product quality stability, and can be matched with an antibiotic for use; besides, the inactivated type lactic acid bacteria have the efficient capacity of being adhered to epithelial cells of intestinal tracts, and are resistant to high temperature, high acid and bile; when the inactivated type lactic acid bacteria and the antibiotic are in synergic use, the problems of a viable bacteria preparation can be safely and effectively solved; and in addition, the inactivated lactic acid bacteria can generate competitive inhibition on harmful microorganisms, adsorb harmful substances and regulate an immune response reaction, so that the immunity of organisms can be enhanced. The preparation method disclosed by the invention has the characteristics of being simple to operate and mild in conditions, and cost reduction, popularization and production are facilitated.

The invention pertains to the technical field of plant genetic engineering, and particularly relates to a promoter CBFP of a key gene CbCBF of a shepherd spurse CBF path and applications thereof. The nucleotide sequence of the promoter is showed as SEQ ID NO:1, wherein CbCBF gene and the promoter thereof contain the nucleotide sequence of the 2283rd basic group in the sequence showed in the sequence table of SEQ ID NO:1, and the nucleotide sequence encodes a transcription factor which is induced by low temperature and can regulate and control a cold-inductive gene. The promoter area contains two ABRE cis-acting elements which are serially arranged, thus being capable of specifically playing a response reaction to low temperature and ABA; and the promoter area contains three MYCRE cis-acting elements CANNTG, thus being capable of being combined with an upstream control element (ICE) (inducer of CBF expression). An inductive expression carrier structured by using the genetic promoter can powerfully induces the expression of a target gene when the plant is stressed by low temperature. The invention also provides the applications of the promoter in cultivating cold-resistant plants, which realizes the breed improvement of crops.

Compositions and methods are disclosed that relate to improved treatments for nervous system diseases and disorders using CNS-implanted semi-permeable biocompatible capsules containing encapsulated pathogen-free xenogeneic choroid plexus (CP) cells that are induced to produce altered (and in certain embodiments increased) levels of one or more cerebrospinal fluid (CSF) components. Capsules are selected as disclosed to be capable of induction of elevated CSF production levels by CP cells that are remarkably (>16 months post implant) long-lived, without eliciting immunological rejection, inflammation or foreign body response reactions.

The present invention relates to a wheat glycogen synthetase kinase gene obtained by utilizing cDNA-AFLP technique to clone the salt-stressed specific expression fragment of wheat salt-tolerant mutant, adopting sequence determinatio Blast identification and RACE method. The total length of said gene is 1573bp, in which its open reading frame (ORF) contains 1143 bp, coding 381 amino acids, its coded protein extensively participates in plant response reaction to drought, ABA induction and light induction, specially participates in salt-stressed signal conduction process. After said gene is cloned into prokaryotic expression vector, it can express out a prokaryotic expression product whose molecular weight is 43.6 KD in the colibacillus, i.e. glycogen synthetase kinase protein. Said invention also provides its application field.

The invention discloses an oncolytic virus and neoantigen combined tumor vaccine and a preparation method thereof. The combined vaccine can be implemented through the following two ways: in the firstway, an immune response reaction generated from an oncolytic virus reagent is used for improving the efficiency for phagocytizing a polypeptide reagent by an antigen presenting cell, increasing the number of T cells which can specifically identify a neoantigen and improving the tumor invasion performance of the T cells so as to enhance the anti-cancer curative effect; and in the second way, a genecoding a tumor neoantigen is inserted into an oncolytic virus vector to massively express the tumor neoantigen, and is combined with the tumor killing performance of the oncolytic virus to further enhance the immune response reaction of a tumor focus local part and improve the invasion degree of the killing type T cell in a tumor tissue, so that the local part can generate an immune response reaction, and generation of an effector cell is stimulated to achieve an anti-cancer effect. Experiment comparison discovers that the first combined vaccine and the second combined vaccine have an excellent tumor inhibition effect, and the second combined vaccine has a remarkable tumor inhibition effect.

The invention relates to a helicobacter pylori dominant antigen assembly based on CD4+T cell immunity and a preparation method. The dominant antigen assembly comprises the following three components and homologous protein of the three components: inosine monophosphate dehydrogenase, type II citrate synthase and urease B subunit, wherein the amino acid sequences are shown as SEQ ID NO.1, SEQ ID NO.2 and SEQ ID NO.3. The screened dominant antigen assembly based on CD4+T cell immunity has the obvious immune protection effect, has the protection effect superior to that of H.pylori holoprotein antigen, has strong capacity of scavenging helicobacter pylori, and causes extremely slight pathological injuries. The three immunity dominant antigens provided by the invention can induce the body to generate strong immune response reaction aiming at antigens, therefore, through the means of inducing the body to generate the response aiming at the immunoprotecive dominant antigens, or directly immunizing the body by adopting the protective antigens, the effective immune protection effect can be achieved on the helicobacter pylori infection, and the scheme can be used for the further study on the preventive and therapeutic polyvaccines of helicobacter pylori.

The invention belongs to the technical field of transition metal detection and discloses a fluorescence probe for detecting three-valent iron ions and a preparation method of the fluorescence probe. The structural formula of the fluorescence probe is represented by a formula (I) (shown in the description). The preparation method comprises the following steps: reacting by virtue of rhodamine B andhydrazine hydrate in an ethanol solution, so as to obtain an intermediate 1; reacting by virtue of the intermediate 1 with chloroacetyl chloride and triethylamine in dichloromethane under an ice bathcondition, so as to obtain an intermediate 2; and adding potassium iodide, 2-methoxy-benzylamine or 2-thienylmethylamine and potassium carbonate into acetonitrile for reaction, so as to obtain a fluorescence probe for detecting Fe<2+>. The preparation method only comprises the three steps, the after-treatment process is simple, the operation is simple, and the product is easily prepared; and the prepared product can be applied to ultraviolet and fluorescent double response reaction detection of Fe<3+> and can be used for detecting Fe<3+> through naked eyes; and the preparation method has the advantages of high response speed and sensitivity and has wide application prospects in the fields of chemical engineering, environment, biological medicines and the like.

The invention discloses a transcription factor capable of improving stress resistance of white birch and protein encoded by the transcription factor, relates to a gene capable of improving the stress resistance of the white birch and the protein encoded by the gene, and aims at providing the transcription factor capable of improving the stress resistance of the white birch and the protein encoded by the transcription factor. A substantial foundation is laid for cultivation and improvement of the white birch variety with high stress resistance in future through a transgenic technology. A nucleotide sequence of the transcription factor capable of improving the stress resistance of the white birch is as shown in SEQ ID NO:1; and an amino acid sequence of the encoded protein is as shown in SEQ ID NO:2. The transcription factor BpWND1 gene capable of improving the stress resistance of the white birch is subjected to obvious inducible expression due to drought and salt stress after being led to the white birch, and the BpWND1 gene participates into the drought-resistant and salt-resistant stress response reaction of the white birch.

An infrared communication type vehicle traffic management system for the traffic management and pass / park toll is composed of vehicle induction unit, lens-CCD camera set, image pick-up cards, license plate image processing unit, infrared communication units on road side and in vehicle, instant response reaction unit, instant response reaction rule library, pass record database, data post-processing unit, and man-vehicle database.

The present invention relates in general to the field of diagnostics, namely to test systems for polypeptides in respect to silicone. More precisely, the present invention, in one embodiment thereof, concerns test systems for identifying and analyzing polypeptides adhering to silicone. Furthermore, the present invention relates to newly identified polypeptides adhering to silicone and to respective uses of such proteins, e.g., in test systems for detecting a response reaction of a mammal to a silicone.

The invention relates to a preparation method of UV770. The preparation method comprises the following steps: (S1) adding (a) 2,2,6,6-tetramethylpiperidine alcohol with the formula shown in the specification and (b) sebacic acid with the formula shown in the specification into a reaction tank, and carrying out fusion mixing; (S2) introducing nitrogen to protect the reaction; (S3) adding titanium catalysts into the reactor, and carrying out thermal response reaction; (S4) cooling reactants to 100-110 DEG C after the reaction is finished, and immediately filtering; and (S5) cooling filtrate, separating out crystals, washing, and further refining rough products to obtain the purified product UV770, or evaporating water to directly obtain the product. The preparation method has the characteristics of high reaction efficiency and convenience in refining.

The invention relates to an FvNCED3 gene used for enhancing the salt tolerance of Fraxinus velutina Torr. and application thereof. The nucleotide sequence of the FvNCED3 gene controlling the salt tolerance of Fraxinus velutina Torr. is as shown in SEQ ID No.1, and the encoded nucleotide sequence of the FvNCED3 gene is as shown in SEQ ID No.2. The FvNCED 3 gene related to plant salt tolerance enhancement is found in Fraxinus velutina Torr. for the first time, and tests prove that the FvNCED 3 gene has good application prospects and particularly has huge potential application value in plant stress tolerance improvement in genetic engineering. Meanwhile, theoretical bases are provided for research of a whole plant stress tolerance gene control network and a stress response reaction mechanism, crop stress resistance is improved through the plant genetic engineering method, and a genetic basis is provided for innovation of new stress tolerance tree species.

The invention discloses an anti-theft intelligent lock which is mounted between a door body and a door frame. The anti-theft intelligent lock comprises a controller, an anti-theft detector, an electric control main lock part and an electric control auxiliary lock part; the anti-theft detector is used for detecting a door lock state, and outputting an abnormal signal when the anti-theft intelligent lock is disassembled violently; the controller receives the abnormal signal and outputs a first alarm signal to the electric control auxiliary lock part; and the electric control auxiliary lock part is controlled to work by the first alarm signal and locks the door body and the door frame together. When a lawbreaker disassembles the anti-theft intelligent lock, an alarm module immediately makes a response reaction and sends a message to a server, and the server sends the message to a mobile terminal to remind a user. When an alarm responses, the control module raises the security level of the anti-theft intelligent lock to the highest, and delays time for the user to adopt measures.

The invention relates to the technical field of medical biology, in particular to a leptospira interrogans DNA (Deoxyribose Nucleic Acid) vaccine as well as a construction method and application thereof. The leptospira interrogans can rapidly invade the inside of human and various animals to cause infection via the skin or the mucous membranes, and result in leptospirosis with the main symptoms of fever, jaundice and bleeding after humans are infected, the leptospirosis is the common zoonosis with wide world distribution and seriously threat the health of the humans and the production of animal husbandry, and China is one of the main countries with the epidemic leptospirosis. The invention provides the leptospira interrogans DNA vaccine, which comprises a eukaryon expression vector and leptospira interrogans LB018 genes, wherein the nucleotide sequence of the LB018 genes is shown as SEQ ID NO: 1. The leptospira interrogans DNA vaccine can effectively induce the immune response reaction of a piggy, which shows that the leptospira interrogans DNA vaccine has a certain immune protection action on the leptospira interrogans of the animals, and the leptospira interrogans DNA vaccine has the advantages of simple production technology, low cost and stable physical and chemical properties, and is convenient for storage and transportation.

The invention discloses a bivalent DNA vaccine connecting peptide and applications thereof. A bivalent DNA vaccine is obtained through the following steps: performing PCR amplification on an HSV-2 glycoprotein gene and an adjuvant gene, introducing enzyme cutting sites, and introducing a connecting peptide (G4S)2IZ(G4S)2 via overlapping PCR. Humoral immunity, humoral immunity and mucosal immunity response reactions triggered by the bivalent DNA vaccine are detected by prime-boost principle immunized mice; a serotype antibody is detected and the severity of illness of each group within 15 days is evaluated by vaginal infection of150LD50 HSV-2. Compared with a univalent DNA vaccine, the recombinant bivalent DNA vaccine generates more intense humoral immunity, humoral immunity and mucosal immunity response reactions and a powerful capability of neutralizing the virus is detected out; high-titer antibodies can be rapidly generated by the immunity reactions and the anamnestic immunity reactions of the recombinant bivalent vaccine, so that a good protection effect on the mice is achieved.

The invention provides N,N'-dicyclohexyl-N-higher fatty acid ureide analogs and pharmaceutical application thereof, relating to therapeutic compounds. A pharmaceutical composition of the N,N'-dicyclohexyl-N-higher fatty acid ureide analogs, and pharmaceutically acceptable salts, solvates, hydrates or carriers thereof can be prepared by a method known by the technical personnel in the technical field. The invention relates to a pharmaceutical composition containing pharmaceutically effective amount of compounds disclosed as general formula I. The composition is used for regulating immune response reaction of the organism, and especially used for preparing organ transplantation immunological rejection inhibitors. The N,N'-dicyclohexyl-N-higher fatty acid ureide analogs can be used for preparing immunomodulating drugs, immunosuppressive drugs, drugs for treating immunological rejection reaction, drugs for treating rheumatic arthritis, anti-inflammatory agents, drugs for treating rheumatalgia, drugs for treating autoimmune disease, drugs for treating fever diseases, organ transplantation immunological rejection inhibitors and the like; and the N,N'-dicyclohexyl-N-higher fatty acid ureide analogs can be used in cytological experiments and experimental animal in-vivo researches.

The invention discloses a swine mycoplasma hyopneumoniae p52 gene recombinant adenovirus and a construction method and an application thereof. In the invention, a p52 gene is cloned into a shuttle vector pShuttle-CMV and is linearized, is electro transformed into BJ5183 fungus to carry out homologous recombination with a skeleton vector pAdEasy-1 contained therein, is mixed with liposome to transfect AD-293 cells to be packaged into complete adenoviruses. After being identified by RT-PCR and indirect immunofluorescence, virus is augmented, and fluid with the virus is collected, Balb / c mice areimmunized, and the immunity result is analyzed. Results show that recombinant adenovirus expressing the p52 gene is constructed successfully; the microorganism preservation number thereof is CGMCC NO. 2986. The recombinant adenovirus can correctly express a target protein and has high potency. Animal experiments prove that the recombinant adenovirus of the invention can generate special humoral immunity and mucosal immunization response reaction.

The invention relates to a water body pollutant detection method, in particular to a rapid detection method for imidacloprid water body pollution by fluorescence quantitative PCR, and belongs to the field of molecular biology. By detecting a response reaction of the aquatic insect choroterpes yixinggensis mitochondrial gene nad4L expression level to imidacloprid, imidacloprid pollution monitoringis conducted, and the method particularly comprises the following steps of 1, culture of the choroterpes yixinggensis of a to-be-detected water sample; 2, total RNA extraction; 3, gel electrophoresisdetection; 4, RNA concentration determination; 5, RNA inversion into cDNA; 6, standard construction and standard curve making; 7, gene expression quantity determination; 8, data analysis. The method is high in flexibility, good in timeliness, high in specificity, easy to implement, and capable of rapidly detecting whether or not the water body is polluted by the imidacloprid.

The invention relates to a helicobacter pylori dominant antigen assembly based on CD4+T cell immunity and a preparation method. The dominant antigen assembly comprises the following three components and homologous protein of the three components: inosine monophosphate dehydrogenase, type II citrate synthase and urease B subunit, wherein the amino acid sequences are shown as SEQ ID NO.1, SEQ ID NO.2 and SEQ ID NO.3. The screened dominant antigen assembly based on CD4+T cell immunity has the obvious immune protection effect, has the protection effect superior to that of H.pylori holoprotein antigen, has strong capacity of scavenging helicobacter pylori, and causes extremely slight pathological injuries. The three immunity dominant antigens provided by the invention can induce the body to generate strong immune response reaction aiming at antigens, therefore, through the means of inducing the body to generate the response aiming at the immunoprotecive dominant antigens, or directly immunizing the body by adopting the protective antigens, the effective immune protection effect can be achieved on the helicobacter pylori infection, and the scheme can be used for the further study on the preventive and therapeutic polyvaccines of helicobacter pylori.

The invention provides an immune agent which is obtained by amplifying staphylococcus aureus enterotoxin B in vitro. The agent essentially comprises CD8<+>NK1.1<+>NKT cell and CD8<+>CD3<-> T cell, and can be used for controlling and curing I type diabetes mellitus. The immune agent is proved that the cells are not reacted with classical antigen stimulators any longer, have lower limitation of major histocmpatible complex (MHC), and restrain the response reaction of normal lymphocyte to antigen, and can be used for anti rejection of organ transplantation. After being frozen and thawed, the immune agent has invariable functions.

The invention relates to a preparation method of a multidimensional multivalence prostatic cancer specificity tumor antigen assembly. Cultured mycobacterium bovis(BCG vaccine BCG) thalli, and throughseparation, refining and purifying, obtained thalli protein is a basic carrier. The effect of the multidimensional multivalence prostatic cancer specificity tumor antigen assembly can mobilize and activate an immune response reaction of an organism autoimmunity system. A nanometer polymer is used as a multi-connection arm, and self modified structure functional groups are utilized. Through a combination manner of covalent bonds or non-covalent bonds, thalli protein and specific prostatic cancer and tumor antigen polypeptide genetic fragments are connected. The multidimensional multivalence three-dimensional configuration bridge linkage mode is finished, and the multidimensional multivalence prostatic cancer specificity tumor antigen assembly having the characteristics of being high in biological safety, clear in specific antigen, high in autosensitization original nature and the like is prepared. The preparation method can be used for preparing a prostatic cancer specificity antibody,is used for pinpoint research, development and production of prostatic cancer vaccines for human, and has favorable wide application prospects.