A kind of bivalent dna vaccine connection peptide and its application

A technology of DNA vaccine and connecting peptides, which is applied in the field of genetic engineering and immunology, can solve the problems of affecting the normal function of genes, not being able to protect men, and the imbalance of gene functions, so as to enhance vaccine immune response, enhance memory immune response, The effect of strong virus neutralization ability
CN103864902BActive Publication Date: 2015-09-30WUHAN INST OF VIROLOGY CHINESE ACADEMY OF SCI
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
WUHAN INST OF VIROLOGY CHINESE ACADEMY OF SCI
Publication Date
2015-09-30

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Abstract

The invention discloses a bivalent DNA vaccine connecting peptide and applications thereof. A bivalent DNA vaccine is obtained through the following steps: performing PCR amplification on an HSV-2 glycoprotein gene and an adjuvant gene, introducing enzyme cutting sites, and introducing a connecting peptide (G4S)2IZ(G4S)2 via overlapping PCR. Humoral immunity, humoral immunity and mucosal immunity response reactions triggered by the bivalent DNA vaccine are detected by prime-boost principle immunized mice; a serotype antibody is detected and the severity of illness of each group within 15 days is evaluated by vaginal infection of150LD50 HSV-2. Compared with a univalent DNA vaccine, the recombinant bivalent DNA vaccine generates more intense humoral immunity, humoral immunity and mucosal immunity response reactions and a powerful capability of neutralizing the virus is detected out; high-titer antibodies can be rapidly generated by the immunity reactions and the anamnestic immunity reactions of the recombinant bivalent vaccine, so that a good protection effect on the mice is achieved.
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Description

technical field

[0001] The invention relates to the fields of genetic engineering and immunology, more specifically to a bivalent DNA vaccine linking peptide, and also relates to the application of the linking peptide in preparing a bivalent DNA vaccine. Background technique

[0002] A bivalent DNA vaccine refers to a fusion DNA molecule that uses gene splicing technology and DNA recombination technology to connect two genes to form a fusion. After the target gene is introduced into the cell, it can be translated into a bifunctional protein molecule alone or in fusion, which can make the body produce specific vaccines. Biological agents for sexual immunity.

[0003] The two genes are connected with a linker (linker) or directly, and the same open reading frame is used to translate a chimeric bifunctional fusion protein molecule. The general method is to delete the stop codon of the first gene, and its reading frame is the same as that of the first gene. The initiation codon...

Claims

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