Inactivated type lactic acid bacteria microecological preparation as well as preparation method and application thereof

A micro-ecological preparation, the technology of inactivating Lactobacillus, applied in the direction of bacteria, application, Lactobacillus used in food preparation, can solve the problems of long-term colonization, loss of vitality, single active ingredient and so on

Inactive Publication Date: 2017-01-11
JIANGSU WECARE BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, probiotic preparations are often easily affected by environmental factors such as production and transportation, resulting in loss of vitality, and live bacterial preparations cannot effectively resist the influence of gastric acid and bile sa...

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] S1, respectively activate Bifidobacterium breve BBr60 and Lactobacillus plantarum Lp90 in test tubes of MRS medium to obtain primary seed liquid, then transfer the primary seed liquid to seed medium to obtain secondary seed liquid, and culture The condition is 38°C, anaerobic culture for 10h. The secondary seed liquid was respectively connected to the fermentation medium for high-density fermentation, the inoculum amount of the secondary seed liquid was 5% when connected to the fermentation medium, the culture condition was 38° C., anaerobic culture was carried out for 12 hours, and the fermentation liquid was collected respectively.

[0029] S2. Centrifuge the fermented liquid separately, collect the cells, add trehalose protective agent to emulsify, and inactivate the obtained emulsified liquid at high temperature for 50 minutes at 80° C., and then carry out vacuum freeze-drying to obtain inactivated probiotic bacteria powder.

[0030] S3, add inactivated bacteria pow...

Embodiment 2

[0032] S1. Activate Bifidobacterium lactis BLa80, Bifidobacterium longum BL21, and Lactobacillus acidophilus LA85 in test tubes of MRS medium respectively to obtain primary seed liquid, and then transfer the primary seed liquid to seed medium respectively The secondary seed solution was obtained, the culture condition was 38° C., and anaerobic culture was carried out for 10 h. The secondary seed liquid was respectively connected to the fermentation medium for high-density fermentation, the inoculum amount of the secondary seed liquid was 5% when connected to the fermentation medium, the culture condition was 38° C., anaerobic culture was carried out for 12 hours, and the fermentation liquid was collected respectively.

[0033] S2. Centrifuge the fermented liquid separately, collect the cells, add trehalose protective agent to emulsify, and inactivate the obtained emulsified liquid at 80° C. for 50 minutes, and then carry out vacuum freeze-drying to obtain inactivated probiotic ...

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PUM

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Abstract

The invention relates to an inactivated type lactic acid bacteria microecological preparation as well as a preparation method and application thereof. The inactivated type lactic acid bacteria microecological preparation comprises inactivated bacteria and auxiliary materials, wherein the inactivated bacteria comprise inactivated bifidobacteria and inactivated bacterium lacticum. The inactivated microecological preparation disclosed by the invention is high in safety, resistant to infection by bacteriophages, free from limit of bacterium quantity, and high in product quality stability, and can be matched with an antibiotic for use; besides, the inactivated type lactic acid bacteria have the efficient capacity of being adhered to epithelial cells of intestinal tracts, and are resistant to high temperature, high acid and bile; when the inactivated type lactic acid bacteria and the antibiotic are in synergic use, the problems of a viable bacteria preparation can be safely and effectively solved; and in addition, the inactivated lactic acid bacteria can generate competitive inhibition on harmful microorganisms, adsorb harmful substances and regulate an immune response reaction, so that the immunity of organisms can be enhanced. The preparation method disclosed by the invention has the characteristics of being simple to operate and mild in conditions, and cost reduction, popularization and production are facilitated.

Description

technical field [0001] The invention relates to the technical field of probiotics, in particular to an inactivated lactic acid bacteria probiotic and its preparation method and application. Background technique [0002] Microecological preparations are living microbial preparations made from normal microorganisms or substances that promote the growth of microorganisms. That is to say, all preparations that can promote the growth and reproduction of normal microflora and inhibit the growth and reproduction of pathogenic bacteria are called "microecological preparations". Its main component, probiotics, is a type of active microorganisms that are beneficial to the host. They are active beneficial microorganisms that colonize the human intestinal tract and reproductive system and can produce definite health effects, thereby improving the host's micro-ecological balance and exerting beneficial effects. Probiotics can help the human body maintain and regulate the balance of inte...

Claims

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Application Information

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IPC IPC(8): A23L33/135A61K35/747A61P37/04A61K35/745
CPCA61K35/745A61K35/747A23V2002/00A23V2400/113A23V2400/519A23V2400/531A23V2400/533A23V2400/169A23V2200/32A61K2300/00
Inventor 方曙光杨帆姜甜占英英冯胜鹏
Owner JIANGSU WECARE BIOTECHNOLOGY CO LTD
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