Rapid detection method for imidacloprid water body pollution by fluorescent quantitative PCR

Abstract

The invention relates to a water body pollutant detection method, in particular to a rapid detection method for imidacloprid water body pollution by fluorescence quantitative PCR, and belongs to the field of molecular biology. By detecting a response reaction of the aquatic insect choroterpes yixinggensis mitochondrial gene nad4L expression level to imidacloprid, imidacloprid pollution monitoringis conducted, and the method particularly comprises the following steps of 1, culture of the choroterpes yixinggensis of a to-be-detected water sample; 2, total RNA extraction; 3, gel electrophoresisdetection; 4, RNA concentration determination; 5, RNA inversion into cDNA; 6, standard construction and standard curve making; 7, gene expression quantity determination; 8, data analysis. The method is high in flexibility, good in timeliness, high in specificity, easy to implement, and capable of rapidly detecting whether or not the water body is polluted by the imidacloprid.

Description

technical field [0001] The invention relates to a water body pollutant detection method, in particular to a rapid detection method for imidacloprid water body pollution by fluorescent quantitative PCR, belonging to the field of molecular biology. Background technique [0002] Imidacloprid belongs to neonicotinoid pesticide, which is a kind of neurotic insecticidal pesticide that can keep insects excited or paralyzed and then die. Its high toxicity to invertebrates, ease of use, flexibility, and long-term persistence make it a class of insecticidal pesticides with a high global market share. This type of pesticide represented by imidacloprid has lethal or sub-lethal effects on terrestrial and macrobenthic animals such as birds and bees, and aquatic invertebrates such as mayfly larvae, causing a decrease in earthworm fecundity and affecting ecological balance. At the same time, due to their good water solubility and high leaching rate, they are easy to enter the aquatic ecosy...

AI Technical Summary

Problems solved by technology

[0003] In order to solve the problem of imidacloprid water pollution detection, the present invention provides a rapid detection method for imidacloprid water pollution by fluorescence quantitative PCR, which measures the mitochondrial gene expression level of the aquatic insect Yixing broad-based mayfia by real-time fluorescence quantitative PCR technology to quickly detect whether the water body is affected by imidacloprid. Pollution, with the characteristics of convenience, high sensitivity, simple operation, etc.

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