Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Recombination interferon with new space conformation and enhanced effect, its preparing method and application

A recombinant interferon and sequence technology, which is applied to the composition of the same substance, the pharmaceutical composition, and the application field of medicine, can solve the problems of inability to use large doses, large side effects, and inability to directly inhibit the secretion of HBsAg and HBeAg, and achieves Reduce toxic side effects, low side effects, and inhibit secretion

Active Publication Date: 2010-05-12
SUPERLAB FAR EAST LTD
View PDF5 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] For the diagnosis of hepatitis B patients, when surface antigen (HBsAg) positive and e antigen (HBeAg) positive are detected, they can be judged as hepatitis B patients. At present, various types of human α-interferon have been used clinically to treat chronic Hepatitis B patients are treated, and its mechanism of action is: interferon binds to the specific receptor of the cell to exert its antiviral DNA and RNA synthesis effect, including the induction of certain enzymes to prevent the replication of the virus in the virus-infected cell. However, in vitro pharmacodynamics of all interferons can only inhibit viral DNA and RNA, and cannot directly inhibit the secretion of HBsAg and HBeAg. Moreover, they cannot be used in large doses due to large side effects.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Recombination interferon with new space conformation and enhanced effect, its preparing method and application
  • Recombination interferon with new space conformation and enhanced effect, its preparing method and application
  • Recombination interferon with new space conformation and enhanced effect, its preparing method and application

Examples

Experimental program
Comparison scheme
Effect test

example 1

[0076] According to the published DNA sequence encoding interferon alfacon-1 and deduced amino acid sequence data (Klein ML, Bartley TD, Lai PH, et al., Structural characterization of recombinant consensus interferon-alpha. Journal of Chromatography, 1988; 454:205-215), see figure 1 , we used Escherichia coli to preferentially express codons (The Wisconsin Package, by Genetics Computer Group, Inc. Copyright 1992, Medison, Wisconsin, USA), and under the condition that the amino acid sequence remained unchanged, molecular design was carried out for its DNA coding sequence, Then the full-length cDNA encoding gene of rSIFN-co was artificially synthesized.

[0077] In order to obtain a large amount of high-purity rSIFN-co protein for research and clinical treatment, we used recombinant DNA technology to clone the full-length cDNA sequence of rSIFN-co into the high-efficiency expression vector of E. coli, and then induced / activated the expression with L-arabinose The regulatory me...

example 2

[0136] Isolation and Purification of Recombinant Interferon (rSIFN-co)

[0137] Fermentation of engineered bacteria

[0138] Inoculate the engineered bacteria in LB medium, 37 ° C, shake the flask (200rpm) and cultivate overnight (about 18 hours), add 50% glycerol with a concentration of 30% to the bacterial culture solution, mix and divide into 1ml each, Store at -20°C as a production strain.

[0139] The production strain was inserted into LB medium (containing 10 g of peptone, 5 g of yeast extract, and 10 g of sodium chloride in 1 L) at a ratio of 1%, cultivated overnight at 37° C. at 200 rpm, and was used as a grade I seed fungus. Grade I seed bacteria are added into RM medium in a ratio of 10% (1L contains 20g of casein, 1mmol / L of magnesium chloride (0.203g), 4g of disodium hydrogen phosphate, 3g of potassium dihydrogen phosphate, 0.5g of sodium chloride, chlorine amine 1g), 37°C, pH 7.0, fermented to OD 600 Add arabinose (20%) to a final concentration of 0.02% to ind...

example 3

[0154] Differences in spatial conformation between recombinant interferon (rSIFN-co) of the present invention and interferon alfacon-1

[0155] The spatial conformation difference between the recombinant interferon (rSIFN-co) of the present invention and interferonalfacon-1 was compared by circular dichroism method.

[0156] Adopt circular dichroism instrument J-500C, set measurement sensitivity to be 2m° / cm, optical path be 0.20cm, measure the circular dichroism spectrum of dried rejuvenated sample in wavelength range 190nm-250nm; Described dried rejuvenated sample contains 30μg / ml Dry Fujin, 5.9mg / ml NaCl, 3.8mg / ml Na 2 PO 4 , pH 7.0. The result is shown in Fig. 6A.

[0157] Adopt circular dichroism instrument J-500C, setting and measuring sensitivity is 2m ° / cm, and optical path is 2cm, and measuring recombinant interferon (rSIFN-co) sample of the present invention is the circular dichroism spectrum in the wavelength range of 250nm-320nm; The above interferon samples co...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention provides recombination interferon (rSIFN-co) or the functional analog with new space conformation, enhanced effect, and low side effect. It is showed by the external pharmacodynamics, itcan not only restrain the DNA replication of the hepatitis b virus, but also restrain the exudation of the surface antigen (HBsAg) and e antigen (HBeAg). The cytology toxicity is 1 / 8 of the clinicalinterferon and the antiviral activity is 5-20 times to the clinical interferon and has a higher, a more board spectrum and longer time biological response reaction, and prevent the tumor hyperplasia and transmission. The invention also provides the synthetic gene code, gene carrier, expression system of the synthetic gene of the recombination interferon or the functional analog. Finally, the invention also provides the preparing method and application thereof.

Description

technical field [0001] The invention relates to the field of bioengineering. Specifically, the present invention relates to a recombinant interferon or its functional equivalent that has a new spatial conformation, enhanced efficacy, lower side effects and can be used in large doses, as well as compositions and pharmaceuticals containing the interferon or its functional equivalent. Use the composition. The present invention also relates to the production method of the recombinant interferon or its functional equivalent, and its application in the preparation of drugs for preventing and treating viral diseases and treating tumors. Background technique [0002] Interferon is a soluble glycoprotein produced by a variety of cells with a wide range of antiviral, antitumor and immunomodulatory effects. Under normal circumstances, there is no interferon in tissues or serum, and cells can be induced to produce interferon only under the action of certain specific factors. Accordin...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): A61K38/21A61P31/12A61P31/16A61P31/18C07K14/555
CPCY02A50/30
Inventor 魏光文
Owner SUPERLAB FAR EAST LTD
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com