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Transcription factor capable of improving stress resistance of white birch and protein encoded by transcription factor

A transcription factor and stress resistance technology, applied in genetic engineering, plant gene improvement, plant peptides, etc., can solve the problems of insignificant effect, long cycle, low success rate, etc.

Inactive Publication Date: 2015-04-29
NORTHEAST FORESTRY UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the traditional methods of variety improvement have the problems of inconspicuous effect, long cycle and low success rate.

Method used

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  • Transcription factor capable of improving stress resistance of white birch and protein encoded by transcription factor
  • Transcription factor capable of improving stress resistance of white birch and protein encoded by transcription factor
  • Transcription factor capable of improving stress resistance of white birch and protein encoded by transcription factor

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Experimental program
Comparison scheme
Effect test

specific Embodiment approach 1

[0021] Embodiment 1: The nucleotide sequence of the transcription factor BpWND1 gene that improves the stress resistance of birch in this embodiment is shown in SEQ ID NO:1.

[0022] The amino acid sequence of the protein encoded by the transcription factor BpWND1 gene that improves the stress resistance of birch in this embodiment is shown in SEQ ID NO:2.

[0023] In this embodiment, the full length of the BpWND1 gene is 1281bp, the encoded protein molecule consists of 426 amino acids (SEQID NO: 2), the isoelectric point (PI) is 6.16, the molecular weight (MW) is 47.98KD, and the negatively charged residue (Asp+ Glu) is 52, the positively charged residue (Arg+Lys) is 44, and the molecular formula is C 2093 h 3226 N 598 o 661 S 20 , the instability coefficient (II) is 54.23, the average hydrophobicity (GRAVY) is -0.688, and the fat index (AI) is 62.96.

specific Embodiment approach 2

[0024] Specific embodiment two: this embodiment tests the transcription factor BpWND1 gene that improves birch stress resistance:

[0025] 1. Introduce restriction sites on the upstream and downstream of the transcription factor BpWND1 gene, and design and synthesize the BpWND1 gene sequence and primers with restriction sites;

[0026] 2. Perform PCR amplification on the transcription factor BpWND1 gene. The PCR reaction program is pre-denaturation at 94°C for 2 minutes; denaturation at 94°C for 30 seconds, annealing at 58°C for 30 seconds, extension at 72°C for 2 minutes, and 30 cycles; finally, extension at 72°C for 7 minutes;

[0027] 3. After the collected DNA fragments were purified, they were ligated with the digested pROK2 vector, and then transformed into Escherichia coli Top10 strain;

[0028] 4. The correct recombinant plasmid (pROK2-BpWND1) identified by PCR verification and sequencing was transferred into the Agrobacterium tumefaciens EHA105 strain by the conductom...

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Abstract

The invention discloses a transcription factor capable of improving stress resistance of white birch and protein encoded by the transcription factor, relates to a gene capable of improving the stress resistance of the white birch and the protein encoded by the gene, and aims at providing the transcription factor capable of improving the stress resistance of the white birch and the protein encoded by the transcription factor. A substantial foundation is laid for cultivation and improvement of the white birch variety with high stress resistance in future through a transgenic technology. A nucleotide sequence of the transcription factor capable of improving the stress resistance of the white birch is as shown in SEQ ID NO:1; and an amino acid sequence of the encoded protein is as shown in SEQ ID NO:2. The transcription factor BpWND1 gene capable of improving the stress resistance of the white birch is subjected to obvious inducible expression due to drought and salt stress after being led to the white birch, and the BpWND1 gene participates into the drought-resistant and salt-resistant stress response reaction of the white birch.

Description

technical field [0001] The invention relates to a gene capable of improving birch resistance and its coded protein. Background technique [0002] White birch (Betula platyphylla Suk.) is a plant of Betula family Betula. It is a deciduous tree with fast growth. It is a pioneer tree species of secondary forest in Northeast China. It has strong cold resistance and acidic soil. It is used in plywood, veneer furniture and pulp wood There are a wide range of applications. Because of its important ecological, ornamental and practical economic value, it has always been listed as one of the important tree species studied by the national science and technology plan. The scope of improving birch varieties is also expanding, and its main goal is to breed new birch varieties that are fast-growing, high-quality and highly resistant. However, the traditional means of variety improvement have the problems of inconspicuous effect, long cycle and low success rate. Genetic improvement using...

Claims

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Application Information

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IPC IPC(8): C12N15/29C07K14/415
Inventor 杨传平胡萍张凯敏王玉成王艳敏
Owner NORTHEAST FORESTRY UNIVERSITY
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