Wheat glycogen synthetase kinase gene

A technology of glycogen synthesis and enzyme kinase, which is applied in the field of life sciences and can solve problems such as agricultural production loss

Inactive Publication Date: 2004-08-25
HEBEI NORMAL UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

There are about 500 million mu of saline soil in my country, including more than 100 million mu of saline cultivated land and more than 300 million mu of

Method used

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  • Wheat glycogen synthetase kinase gene
  • Wheat glycogen synthetase kinase gene
  • Wheat glycogen synthetase kinase gene

Examples

Experimental program
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Embodiment

[0059] The wheat glycogen synthase kinase gene provided by the present invention has a full length of 1573bp, wherein the open reading frame (ORF) contains 1143bp and encodes 381 amino acids. The protein encoded by it is widely involved in plant response to drought, salt stress, ABA induction, and light induction. etc., especially its participation in the salt stress signal transduction process. The gene is cloned into a prokaryotic expression vector, and a prokaryotic expression product with a molecular weight of 43.6KD, namely glycogen synthase kinase protein, can be expressed in Escherichia coli.

[0060] The above-mentioned glycogen synthase kinase gene and its expression product can be obtained by the following steps:

[0061] Using the F1 anther culture of Pu Nong 3665 crossed with Bainong 3039, EMS mutagenesis and repeated screening for salt tolerance were obtained from the same

[0062] A wheat salt-tolerant mutant RH8706-49 (salt-tolerant index ≥ 1.3) and a salt-sens...

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Abstract

The present invention relates to a wheat glycogen synthetase kinase gene obtained by utilizing cDNA-AFLP technique to clone the salt-stressed specific expression fragment of wheat salt-tolerant mutant, adopting sequence determinatio Blast identification and RACE method. The total length of said gene is 1573bp, in which its open reading frame (ORF) contains 1143 bp, coding 381 amino acids, its coded protein extensively participates in plant response reaction to drought, ABA induction and light induction, specially participates in salt-stressed signal conduction process. After said gene is cloned into prokaryotic expression vector, it can express out a prokaryotic expression product whose molecular weight is 43.6 KD in the colibacillus, i.e. glycogen synthetase kinase protein. Said invention also provides its application field.

Description

technical field [0001] The invention relates to a wheat glycogen synthase kinase TaGSK1 (Triticum asetivum Glycogen SynthaseKinase1) gene, which belongs to the technical field of life science. Background technique [0002] Studies have shown that the most important factor affecting the growth and yield of crops is environmental stress, among which salt stress and drought stress are the most serious. According to statistics, from 1939 to 1978, among various environmental factors affecting crop yield, the yield reduction caused by drought and salinity was as high as 40%. There are about 500 million mu of saline soil in my country, including more than 100 million mu of saline cultivated land and more than 300 million mu of saline wasteland to be reclaimed and utilized. The area of ​​secondary salinized soil is still expanding, causing heavy losses to agricultural production . How to alleviate the impact of salt stress on agricultural production, so that large areas of saline-a...

Claims

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Application Information

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IPC IPC(8): A01H5/00C07H21/04C12N15/29C12N15/54C12N15/70C12Q1/68
Inventor 沈银柱黄占景马闻师陈桂平徐涛
Owner HEBEI NORMAL UNIV
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