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FvNCED3 gene used for enhancing salt tolerance of Fraxinus velutina Torr. and application thereof

A fluffy white wax and salt tolerance technology, applied in the field of molecular biology, can solve problems such as unseen biosynthesis pathways, and achieve the effect of improving crop stress resistance

Active Publication Date: 2016-03-16
BINZHOU UNIV
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  • Abstract
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  • Application Information

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Problems solved by technology

However, there are no reports about the ABA biosynthesis pathway and the related synthetic gene NCED of C. tomentosa

Method used

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  • FvNCED3 gene used for enhancing salt tolerance of Fraxinus velutina Torr. and application thereof
  • FvNCED3 gene used for enhancing salt tolerance of Fraxinus velutina Torr. and application thereof
  • FvNCED3 gene used for enhancing salt tolerance of Fraxinus velutina Torr. and application thereof

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Embodiment

[0024] 1. Experimental method and steps

[0025] 1.1 Handling of materials

[0026] The 1-month-old A. tomentosa seedlings sown in the same substrate and with consistent growth were used as materials. The A. tomentosa seedlings were treated with 200mM NaCl for 0h, 12h, 24h, and 48h, respectively, and each group had three biological replicates. Fluorescent quantitative PCR method for gene expression pattern analysis, 2 -ΔΔCt method to calculate the relative expression of genes.

[0027] 1.2 Gene cloning

[0028] Weigh 10 mg of the above-mentioned treated materials and place them in a liquid nitrogen precooled mortar and grind them fully until they are powdery; transfer the ground powders into a 1.5ml centrifuge tube with the extract. Then, according to the operating requirements of the E.Z.N.ATotalRNAKit kit manual, the total RNA of each tissue of the above-mentioned processed white wax was extracted respectively. Then prepare 1.2wt% agarose gel electrophoresis to detect t...

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Abstract

The invention relates to an FvNCED3 gene used for enhancing the salt tolerance of Fraxinus velutina Torr. and application thereof. The nucleotide sequence of the FvNCED3 gene controlling the salt tolerance of Fraxinus velutina Torr. is as shown in SEQ ID No.1, and the encoded nucleotide sequence of the FvNCED3 gene is as shown in SEQ ID No.2. The FvNCED 3 gene related to plant salt tolerance enhancement is found in Fraxinus velutina Torr. for the first time, and tests prove that the FvNCED 3 gene has good application prospects and particularly has huge potential application value in plant stress tolerance improvement in genetic engineering. Meanwhile, theoretical bases are provided for research of a whole plant stress tolerance gene control network and a stress response reaction mechanism, crop stress resistance is improved through the plant genetic engineering method, and a genetic basis is provided for innovation of new stress tolerance tree species.

Description

technical field [0001] The invention relates to a FvNCED3 gene for enhancing the salt tolerance of white wax tomentosa and its application, belonging to the technical field of molecular biology. Background technique [0002] Fraxinus velutina Torr. is an excellent tree species for afforestation and urban landscaping in saline-alkali land, and it is also a commonly used sand-fixing tree species. However, there are few reports on the molecular mechanism of stress tolerance of white wax tomentosa at present, and there are fewer natural good genes isolated from it, and the functions of most of its genes are still unknown. Therefore, it is necessary to strengthen the molecular biology research on representative woody plants such as A. tomentosa, and excavate the key stress tolerance genes, so as to provide a theoretical basis and new genetic resources for the genetic improvement of plant stress tolerance traits. [0003] Abscisic acid (ABA) is an important plant hormone that pla...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/53C12N9/02C12N15/82A01H5/00
CPCC12N9/0069C12N15/8273C12Y113/11051
Inventor 李田孙景宽陆兆华刘京涛夏江宝宋爱云
Owner BINZHOU UNIV
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