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Anti-trichina small heat shock protein egg yolk antibody as well as preparation method and application thereof

A technology of small heat shock protein and yolk antibody, applied in the field of biomedicine, can solve problems such as difficulties in clinical promotion and application, and achieve the effects of enhancing animal immunity, facilitating large-scale industrial production and low cost

Pending Publication Date: 2022-03-11
JILIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, there have been some relevant research reports on the anti-tumor effects of polyclonal antibodies and monoclonal antibodies against Trichinella spiralis and cancer-related gene protein antigens. Moreover, the mouse serum and monoclonal antibodies prepared against these related protein antigens have achieved certain anti-tumor effects in mouse animal experiments, but the antibodies used above are all injected, and there are great difficulties in clinical promotion and application.
There is no report on the oral application of poultry yolk antibody to cancer-related gene protein antigen of Trichinella spiralis and tumor

Method used

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  • Anti-trichina small heat shock protein egg yolk antibody as well as preparation method and application thereof
  • Anti-trichina small heat shock protein egg yolk antibody as well as preparation method and application thereof
  • Anti-trichina small heat shock protein egg yolk antibody as well as preparation method and application thereof

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Effect test

Embodiment 1

[0031] Expression of antigen gene sHSPs associated with Trichinella spiralis and Lewis lung cancer

[0032] The primers were designed according to the specific gene sequence of Trichinella spiralis small heat shock proteins (sHSPs) (DQ986457.1), and the nucleotide sequences of the primers were:

[0033] Upstream primer: 5'-ATGGCCTTGACTGCGTGGTATAATC-3';

[0034] Downstream primer: 5'-TCATCTCTTCTGAGCAGCCGGAGC-3';

[0035] The enzyme cutting sites were: GGATCC (BamHⅠ); GTCGAC (SalⅠ); using the cDNA of Trichinella spiralis muscle larvae as a template, the sHSPs gene was amplified by PCR and ligated with the cloning vector pMD-18T, named pMD-18T-sHSPs. After sequencing verification, pMD-18T-sHSPs and empty vector pET-32a(+) were digested with double enzymes respectively, pMD-18T-sHSPs and pET-32a(+) recovered from the gel were ligated with T4 DNA ligase, and finally transformed into BL- 21 (DE3), the expression of the recombinant bacteria with correct sequencing was induced by IP...

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Abstract

The invention provides an anti-trichina small heat shock protein (sHSPs) chicken egg yolk antibody and a preparation method thereof, and also discloses a medical application of the egg yolk antibody in tumor resistance. The traditional Chinese medicine composition has the characteristics of enhancing animal immunity, being non-toxic and the like, can be used as an oral preparation medicine for treating tumors, and can be prepared into oral liquid and the like as food for preventing tumors; the egg yolk antibody for poultry such as chicken can be orally taken, is convenient to use, non-toxic, low in cost, safe and convenient for large-scale industrialized production.

Description

Technical field: [0001] The invention provides an anti-trichinella small heat shock protein egg yolk antibody, relates to an anti-trichinella spiralis and Lewis lung cancer-related gene protein antigen sHSPs egg yolk antibody, in particular discloses an anti-trichinella spiralis and Lewis lung cancer-related gene protein antigen sHSPs chicken egg yolk Antibody and preparation method; meanwhile, the medical application of yolk antibody in anti-tumor is provided, which belongs to the technical field of biomedicine. Background technique: [0002] Trichinella ( Trichinella spiralis ) is a zoonotic parasite in which adults live in the small intestine of mammals and larvae live in muscle tissue. Current studies have found that Trichinella spiralis can not only cause trichinellosis, but infection by Trichinella spiralis can also improve the anti-tumor ability of the host. At present, there have been some relevant research reports on the anti-tumor effects of polyclonal antibodies...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/12C07K16/02C07K16/18A61K39/395A61P35/00
CPCC07K14/4354C07K16/02C07K16/18A61P35/00C07K2317/11A61K2039/505
Inventor 张西臣岳涛涛张楠李建华宫鹏涛王瑜
Owner JILIN UNIV
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