A kind of multiple dpo-pcr primer combination and method for detecting Trichinella spiralis, Toxoplasma gondii and Schistosoma

A DPO-PCR, primer combination technology, applied in microorganism-based methods, biochemical equipment and methods, recombinant DNA technology, etc., can solve the problems of complex system, low stability, limited use, etc., to achieve high throughput, Good specificity and improved detection efficiency
CN106701985BInactive Publication Date: 2020-08-04海南出入境检验检疫局检验检疫技术中心
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
海南出入境检验检疫局检验检疫技术中心
Publication Date
2020-08-04
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention provides a multiple DPO-PCR (dual priming oligonucleotide-polymerase chain reaction) primer combination for detecting trichinella spiralis, toxoplasma gondii and schistosome. The sequences of the primer combination are as shown in SEQ ID NO:1 to SEQ ID NO:6 correspondingly. The invention also provides a multiple DPO-PCR method for detecting the trichinella spiralis, the toxoplasma gondii and the schistosome. By adopting the method, qualitative detection on the muscular tissue or viscera sample of animals suffering from diseases can be realized. Experiments indicate that the detection method provided by the invention has high specificity, high flux and high speed, the detection efficiency is improved, and a more effective method is provided for detecting three animal origin parasitic zoonoses of the trichinella spiralis, the toxoplasma gondii and the schistosome.
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Description

technical field

[0001] The invention belongs to the technical field of biological detection, and in particular relates to a combination of primers and a method for simultaneously detecting Trichinella spiralis, Toxoplasma gondii and Schistosoma japonicum based on multiplex DPO-PCR. Background technique

[0002] Trichinella spiralis is a serious food-borne zoonotic parasitic nematode, which can infect more than 150 mammals including humans and is distributed worldwide. Trichinellosis infection comes from ingestion of raw or undercooked pork or other animal meat containing trichinella cysts, and human infection with trichinellosis can cause death. It is a zoonotic parasitic disease that seriously endangers public health safety , so it is a mandatory item in meat hygiene inspection.

[0003] Toxoplasma gondii is an obligate intracellular parasitic protozoan that can infect almost all nucleated cells of warm-blooded animals, and is a zoonotic parasitic disease that is widesprea...

Claims

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