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Method for detecting lactoperoxidase in raw milk using test paper

A lactoperoxidase and detection method technology, applied in the field of analysis and testing of lactoperoxidase in raw milk, can solve the problems of high cost, inability to realize online detection, cumbersome operation, etc., and achieve low detection cost and high detection speed Fast, easy-to-test effects

Inactive Publication Date: 2015-03-11
NORTHEAST AGRICULTURAL UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to solve the problems of cumbersome operation and the need for professional technicians to operate, high cost, or inability to realize online detection in the existing analysis and test method of lactoperoxidase content, and to provide a fast , convenient detection method for lactoperoxidase

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  • Method for detecting lactoperoxidase in raw milk using test paper
  • Method for detecting lactoperoxidase in raw milk using test paper
  • Method for detecting lactoperoxidase in raw milk using test paper

Examples

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Embodiment 1

[0043] Example 1 Rapid detection of lactoperoxidase

[0044] The test paper detection method of lactoperoxidase in the raw material milk of this embodiment is carried out according to the following steps:

[0045] (1) Preparation of test paper soaking solution: respectively prepare 0.1mol / L citric acid-0.2mol / L disodium hydrogen phosphate buffer solution with a pH of 5.0, a TMB solution with a concentration of 22mmol / L, and Tween-20 with a mass fraction of 0.5% Solution: Mix the above three prepared solutions according to the volume ratio of buffer solution: TMB solution: surfactant solution = 40:1:2, shake for 3 minutes and mix well, which is the test paper soaking solution;

[0046] (2) Prepare H 2 o 2 Solution: prepare H with a concentration of 0.6mmol / L 2 o 2 solution.

[0047](3) Preparation of test paper: Soak the cut medium-speed quantitative filter paper strip (3cm×5cm) in the soaking solution prepared in step (1) for 1 to 3 minutes. After it is completely soaked,...

Embodiment 2

[0052] Example 2 Rapid detection of lactoperoxidase

[0053] The analysis and testing method of lactoperoxidase in the raw material milk of the present embodiment is carried out according to the following steps:

[0054] (1) Preparation of test paper soaking solution: prepare respectively a 0.1mol / L citric acid-0.2mol / L disodium hydrogen phosphate buffer solution with a pH of 4.8, a TMB solution with a concentration of 20mmol / L, and a CTAB solution with a mass fraction of 0.3%; Mix the above three prepared solutions according to the volume ratio of buffer solution: TMB solution: surfactant solution = 45:1.5:1, shake for 3 minutes and mix well, which is the test paper soaking solution;

[0055] (2) Prepare H 2 o 2 Solution: Prepare H with a concentration of 0.8mmol / L 2 o 2 solution.

[0056] (3) Preparation of test paper: Soak the cut medium-speed quantitative filter paper strip (3cm×5cm) in the soaking solution prepared in step (1) for 1 to 3 minutes. The lactoperoxidase...

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Abstract

The invention discloses a method for detecting lactoperoxidase in raw milk using test paper. The method includes the following steps: preparing a reaction reagent; conducting test paper reaction; establishing detection standards; detecting lactoperoxidase in a milk sample. The method provided by the invention has the advantages that the detection speed is high, and a quantitative detection of lactoperoxidase can be finished in a few seconds at the soonest; the content of lactoperoxidase in raw milk can be quantitatively detected in 3 minutes; the method is suitable for the online detection of lactoperoxidase; the detection cost is low; the detection result is accurate, and the lowest detected concentration is 1-2 mg / L; the requirements for the environment are low; the detection operations are simple and convenient.

Description

technical field [0001] The invention relates to the field of biochemical detection, in particular to a method for analyzing and testing lactoperoxidase in raw milk. Background technique [0002] Lactoperoxidase (Lactoperoxidase, LP) is a kind of peroxidase, which consists of a polypeptide chain, including 612 amino acid residues, with a molecular weight of about 78kDa. The enzyme is a hydrogen peroxide (H 2 o 2 ) oxidoreductases, as one of the endogenous enzymes in emulsion, widely exist in mammalian mammary glands, salivary glands, lacrimal glands and their secretions. In milk, LP is the enzyme whose content is second only to xanthine oxidase, and its concentration in milk is about 30mg / L. The thermal stability of LP is relatively high. de Wit et al. found that LP in milk is completely inactivated and needs to be kept at 78°C for 15s; Marks et al. proved that ordinary pasteurization cannot inactivate LP in milk. It is still active after 15s of pasteurization. Therefore,...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/78
Inventor 姜瞻梅许晓曦车红霞刘丽丽辛岩
Owner NORTHEAST AGRICULTURAL UNIVERSITY
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