Copper ion detection method based on methanobactin functional gold nanoparticles

Abstract

The invention provides a copper ion detection method based on methanobactin functional gold nanoparticles, which relates to a method of detecting trace copper ions in a sample. The method comprises the following steps: firstly, methanobactin is used to catalyze hydrogen tetrachloroaurate hydrate for in-situ synthesis of a methanobactin functional gold nanoparticle solution; a pretreated control sample and a to-be-detected sample are then added to the methanobactin functional gold nanoparticle aqueous solution, after standing for a period of time, the color of the to-be-detected sample changesinto blue-violet while the color of the control sample is unchanged, and thus, the detected sample is proved to contain copper ions. The method uses the specific combination between methanobactin andcopper to realize copper ion detection, the specificity is strong, the detection result can be distinguished by naked eyes, no complicated operation step and no large instrument is needed, and quick copper ion detection can be realized.

Description

technical field

[0001] The invention relates to the technical field of copper ion detection, in particular to a copper ion detection method based on methanotrophin functionalized nano-gold. Background technique

[0002] Copper ion is an essential trace element for the human body and plays an important role in maintaining the life activities of the body. However, excessive intake of copper can cause gastrointestinal dysfunction, liver and kidney function damage, and even life-threatening in severe cases. Not only that, excessive copper ions will also pollute the environment, destroy the structure and function of the aquatic ecosystem, and interfere with the absorption, transportation and accumulation of nutrients by plants. Therefore, it is necessary to develop a method for detecting trace copper ions in food and environment.

[0003] At present, a variety of methods including atomic absorption spectroscopy, X-ray fluorescence spectroscopy, and microprobes have been develop...

Examples

Embodiment 1

[0027] A copper ion detection method based on methanotrophin functionalized nano-gold, comprising the following steps:

[0028] (1) Sample pretreatment: add trichloroacetic acid to the blank control sample and the sample to be tested, centrifuge at a speed of 10000r / min for 10min after 20min, take the supernatant, and add NaOH to the supernatant until the pH is 7. Obtain pretreated blank control samples and samples to be tested;

[0029] The amount of trichloroacetic acid added in step (1) is 1 / 5 of the volume of the sample to be tested; the control sample is a blank control without copper ions.

[0030] (2) Preparation of methanotrophin functionalized gold nanoparticles: take a certain concentration of methanobacterin solution, add concentration of 1 × 10 -4 ~1×10 -6 mol / L chloroauric acid solution, reacted in a water bath at a certain temperature for 30-120 minutes to obtain a methane-oxidizing bacterin functionalized nano-gold solution;

[0031] In step (2), the mass rat...

Embodiment 2

[0036] A copper ion detection method based on methanotrophin functionalized nano-gold, comprising the following steps:

[0037] (1) Sample pretreatment: add trichloroacetic acid to the blank control sample and the sample to be tested, centrifuge at a speed of 10000r / min for 10min after 20min, take the supernatant, and add NaOH to the supernatant until the pH is 7. Obtain pretreated blank control samples and samples to be tested;

[0038] The amount of trichloroacetic acid added in step (1) is 1 / 5 of the volume of the sample to be tested; the control sample is a blank control without copper ions.

[0039] (2) Preparation of methanotrophin functionalized gold nanoparticles: take a certain concentration of methanobacterin solution, add concentration of 1 × 10 -4 ~1×10 -6 mol / L chloroauric acid solution, reacted in a water bath at a certain temperature for 30-120 minutes to obtain a methane-oxidizing bacterin functionalized nano-gold solution;

[0040]In step (2), the mass rati...

Embodiment 3

[0045] A copper ion detection method based on methanotrophin functionalized nano-gold, comprising the following steps:

[0046] (1) Sample pretreatment: add trichloroacetic acid to the blank control sample and the sample to be tested, centrifuge at a speed of 10000r / min for 10min after 20min, take the supernatant, and add NaOH to the supernatant until the pH is 7. Obtain pretreated blank control samples and samples to be tested;

[0047] The amount of trichloroacetic acid added in step (1) is 1 / 5 of the volume of the sample to be tested; the control sample is a blank control without copper ions.

[0048] (2) Preparation of methanotrophin functionalized gold nanoparticles: take a certain concentration of methanobacterin solution, add concentration of 1 × 10 -4 ~1×10 -6 mol / L chloroauric acid solution, reacted in a water bath at a certain temperature for 30-120 minutes to obtain a methane-oxidizing bacterin functionalized nano-gold solution;

[0049] In step (2), the mass rat...

technical field

[0001] The invention relates to the technical field of copper ion detection, in particular to a copper ion detection method based on methanotrophin functionalized nano-gold. Background technique

[0002] Copper ion is an essential trace element for the human body and plays an important role in maintaining the life activities of the body. However, excessive intake of copper can cause gastrointestinal dysfunction, liver and kidney function damage, and even life-threatening in severe cases. Not only that, excessive copper ions will also pollute the environment, destroy the structure and function of the aquatic ecosystem, and interfere with the absorption, transportation and accumulation of nutrients by plants. Therefore, it is necessary to develop a method for detecting trace copper ions in food and environment.

[0003] At present, a variety of methods including atomic absorption spectroscopy, X-ray fluorescence spectroscopy, and microprobes have been develop...

Examples

Embodiment 1

[0027] A copper ion detection method based on methanotrophin functionalized nano-gold, comprising the following steps:

[0028] (1) Sample pretreatment: add trichloroacetic acid to the blank control sample and the sample to be tested, centrifuge at a speed of 10000r / min for 10min after 20min, take the supernatant, and add NaOH to the supernatant until the pH is 7. Obtain pretreated blank control samples and samples to be tested;

[0029] The amount of trichloroacetic acid added in step (1) is 1 / 5 of the volume of the sample to be tested; the control sample is a blank control without copper ions.

[0030] (2) Preparation of methanotrophin functionalized gold nanoparticles: take a certain concentration of methanobacterin solution, add concentration of 1 × 10 -4 ~1×10 -6 mol / L chloroauric acid solution, reacted in a water bath at a certain temperature for 30-120 minutes to obtain a methane-oxidizing bacterin functionalized nano-gold solution;

[0031] In step (2), the mass rat...

Embodiment 2

[0036] A copper ion detection method based on methanotrophin functionalized nano-gold, comprising the following steps:

[0037] (1) Sample pretreatment: add trichloroacetic acid to the blank control sample and the sample to be tested, centrifuge at a speed of 10000r / min for 10min after 20min, take the supernatant, and add NaOH to the supernatant until the pH is 7. Obtain pretreated blank control samples and samples to be tested;

[0038] The amount of trichloroacetic acid added in step (1) is 1 / 5 of the volume of the sample to be tested; the control sample is a blank control without copper ions.

[0039] (2) Preparation of methanotrophin functionalized gold nanoparticles: take a certain concentration of methanobacterin solution, add concentration of 1 × 10 -4 ~1×10 -6 mol / L chloroauric acid solution, reacted in a water bath at a certain temperature for 30-120 minutes to obtain a methane-oxidizing bacterin functionalized nano-gold solution;

[0040]In step (2), the mass rati...

Embodiment 3

[0045] A copper ion detection method based on methanotrophin functionalized nano-gold, comprising the following steps:

[0046] (1) Sample pretreatment: add trichloroacetic acid to the blank control sample and the sample to be tested, centrifuge at a speed of 10000r / min for 10min after 20min, take the supernatant, and add NaOH to the supernatant until the pH is 7. Obtain pretreated blank control samples and samples to be tested;

[0047] The amount of trichloroacetic acid added in step (1) is 1 / 5 of the volume of the sample to be tested; the control sample is a blank control without copper ions.

[0048] (2) Preparation of methanotrophin functionalized gold nanoparticles: take a certain concentration of methanobacterin solution, add concentration of 1 × 10 -4 ~1×10 -6 mol / L chloroauric acid solution, reacted in a water bath at a certain temperature for 30-120 minutes to obtain a methane-oxidizing bacterin functionalized nano-gold solution;

[0049] In step (2), the mass rat...

Images