105results about How to "Realize qualitative detection" patented technology

The invention discloses a visible and reversible fluorescent probe which comprises a cyanine fluorescent group and a benzothiazole group, and the general formula of the probe is shown in the description. The preparation method of the reversible fluorescent probe comprises the following steps: (1) dropwise adding phosphorus oxychloride into salicylaldehyde and paraformaldehyde for reaction, so as to obtain a product 1; (2) enabling the product 1 and hexamethylene-tetramine to react, so as to obtain a product 2; (3) enabling the product 2 and 2-aminobenzenethiol to react at the room temperature, so as to obtain a product 3; (4) enabling the product 3 and a compound 4 to react, so as to obtain the visible and reversible fluorescent probe. According to the ratiometric fluorescent probe provided by the invention, obvious color variation can be found out under natural light or an ultra-violet lamp, and the qualitative detection of sulfur dioxide gas can be implemented under natural light or a hand-held ultra-violet lamp, so that the operation is simple, high convenience and quickness are achieved, and the effect is remarkable; the fluorescent probe can effectively prevent interference from other impurities in samples, and is excellent in selectivity; in addition, the cumbersome pre-processing process of samples is avoided, so that the detection efficiency is high.

The invention discloses an immunochromatography detection card for detecting fluorescent microspheres of ketamine and methyl amphetamine and a preparation method thereof. The detection card is a double-card type, wherein one card is used for detecting the ketamine and the other is used for detecting the methyl amphetamine so as to realize the simultaneous detection of the ketamine and the methyl amphetamine. The detection card sequentially comprises a filter paper, a sample pad, a glass fiber film, a pyroxylin film and a drinking paper; antibodies marked by fluorescent microspheres are sprayedon the glass fiber film; and a detection zone and a quality-control zone are respectively fixed on the pyroxylin film. The invention adopts a nucleocapsid double-structure shining nano-particle compounded by silicon dioxide and fluorescent substance as a mark, and also adopts immunochromatography technology to realize the quick immunoassay of the ketamine and the methyl amphetamine. When in detection, an optimal excitation light source of the fluorescent microsphere is adopted to carry out excitation, and then whether the fluorescent substance exists on detection line can be detected by nakedeye after excited fluorescence passes through a spectral filter device. The detection card has the advantages of high sensitivity, quick detection performance, convenient operation and economical andpractical property.

The invention discloses a fluorescent microsphere immunochromatography detection card for detecting enrofloxacin and a preparation method thereof. The detection card sequentially comprises filter paper, a sample pad, a glass fiber film, a nitrocellulose film and absorbent paper, wherein a fluorescent microsphere-labeled antibody is sprayed on the glass fiber film; a detection area and a quality control area are fixed on the nitrocellulose film; a conjugate of enrofloxacin and carrier protein is sprayed in the detection area; and an anti-mouse antibody is sprayed in the quality control area. By taking core-shell bistructure luminous nano particles complexed by silica and fluorescent substances as labels and adopting immunochromatography in a competition blocking mode, the invention realizes rapid immunoassay of the enrofloxacin. In the detection process, an optimized excitation light source of the fluorescent microsphere is adopted for excitation, the emitted fluorescence passes through a light filter device, and that whether a detection line is provided with fluorescent substances is observed by naked eyes. The invention has the characteristics that: the detection card has high sensitivity and is rapid in detection, convenient to operate, and economic and practical.

The invention discloses a primer probe combination for identifying four components of canine animal origin. The primer probe combination comprises a pair of universal primers suitable for foxes, minks, raccoon dogs and dogs, four peculiar probes for the foxes, the minks, the raccoon dogs and the dogs, an interior label and an interior label probe. The invention further discloses a kit and a multiple real-time fluorescence PCR (polymerase chain reaction) detection method. The universal primers and the peculiar probes are high in sensitivity and accuracy, false-negative results can be indicated effectively during origin detection of the foxes, the minks, the raccoon dogs and the dogs, the primer probe combination has multiple advantages of accuracy, stability, simplicity in operation, high sensitivity, specificity and flux and the like, and a new approach for identification of components of animal origin in feed and food is explored.

The invention relates to the field of the biological technology and the medical examination technology, and provides a COVID-19 nucleic acid testing primer probe composition, a COVID-19 nucleic acid testing kit and a COVID-19 nucleic acid testing method for solving the problems of low accuracy, poor specificity and low sensitivity of a traditional virus nucleic acid testing method. The COVID-19 nucleic acid testing primer probe composition comprises a first primer probe group, a second primer probe group and a third primer probe group, wherein the first primer probe group comprises a forward primer ORF1ab-F, a probe ORF1ab-P and a reverse primer ORF1ab-R; the second primer probe group comprises a forward primer N-F, a probe N-P and a reverse primer N-R; and the third primer probe group comprises a forward primer Rpp30-F, a probe Rpp30-P and a reverse primer Rpp30-R. According to the primer probe composition disclosed by the invention, through artful design of an amplification primer pair and a detection probe, mutual interference between a plurality of primer pairs and corresponding detection probes is avoided. The kit has a simple structure, an interior label is used for monitoring a collection, transportation and extraction process of a sample to be detected, and the false negative of a detection result is avoided.

The invention provides a detection method of indomethacin. The detection method comprises the following steps: producing and providing indomethacin tablets with different mass gradients; under the same pre-set condition, obtaining characteristic absorption peaks of the indomethacin tablets to terahertz wave radiation under the different mass gradients; radiating a sample to be detected by adoptingterahertz waves and obtaining the characteristic absorption peak of the sample to be detected to the terahertz wave radiation; judging whether the characteristic absorption peak of the sample to be detected to the terahertz wave radiation and the characteristic absorption peaks of the indomethacin tablets to the terahertz wave radiation under the different mass gradients are similar or not underpre-set conditions; if so, judging that the sample to be detected contains the indomethacin. According to the detection method provided by the invention, a manner of radiating the indomethacin tabletsby utilizing terahertz waves is utilized so that the problems of a traditional method of sample damages, high danger and high cost are overcome; the sample is produced by adopting a tabletting methodso that the speed is more rapid when a terahertz detection technology is used for detecting in the future and an obtained result is more accurate and reliable.

The invention discloses a character guided wave based butt-weld defect detection system and a detection method thereof. The detection system is composed of a character guided wave transceiving host, a character guided wave sensor and an upper computer; the character guided wave sensor is horizontally arranged on the upper end face of a detected welding line along the direction of the detected welding line, a signal input end and a signal output end of a sensor of the character guided wave transceiving host are respectively connected with the character guided wave sensor, a communication serial port is connected with the upper computer, and data processing analysis software and a database are installed on the upper computer. The detection method includes: adopting a ratio modal analysis method to separate modal wave packets, and selecting specific modals to have defects positioned according to a pulse-echo method; extracting energy information by the aid of a frequency diagram with smoothness being Wigner-Ville and evaluating sizes of the defects; extracting wave characters and inquiring the waveform comparison database to judge types of the defects. By the arrangement, in-service detection can be realized, and the system is high in detection efficiency, low in cost, portable, especially suitable for rapid and efficient detection for long-distance welding lines of large equipment structures and high in automation degree.

The invention provides an LC-Q-TOF/MS detection technology for 544 pesticide residues in kernel fruit. In a TOF/MS mode, the LC-Q-TOF/MS detection technology separately determines the retention time of each pesticide standard under designated gas chromatography-mass spectrography conditions, determines the ionization form and chemical formula of a compound under the condition of an ESI source and acquires the accurate mass number of the parent ion of each compound so as to form a TOF/MS database. In a Q-TOF/MS mode, the LC-Q-TOF/MS detection technology separately acquires the fragment ion mass spectrum of each pesticide standard under the condition of three to five different collision energies and introduces acquired information into PCDL software so as to form a Q-TOF/MS database. Through comparison of the retention time, mass spectrometry information and tandem mass spectrometry information of a kernel fruit sample, whether the sample contains pesticide residues or not can be determined; and compounds with high primary scores are subjected to secondary confirmation, and if secondary scores are high, it is confirmed that related pesticide residues are detected. The LC-Q-TOF/MS detection technology provided by the invention has the advantages of fastness, high throughput, high accuracy, high reliability and the like, and can accurately screen pesticides in the kernel fruit.

The invention belongs to the technical fields of organic synthesis and iron-ion detection and particularly relates to N-butyl-4-hydroxyl-1,8-naphthalimide-3-formaldehyde-(2-pyridine)hydrazone and an application. The preparation method comprises the following steps of: dissolving N-butyl-4-hydroxyl-1,8-naphthalimide-3-formaldehyde and 2-hydrazinopyridine into methanol, heating and refluxing, cooling till the reaction is ended, carrying out suction filtration, and washing with the methanol to obtain the N-butyl-4-hydroxyl-1,8-naphthalimide-3-formaldehyde-(2-pyridine)hydrazone. The invention alsoprovides a qualitative and quantitative detection method of Fe3+ by the N-butyl-4-hydroxyl-1,8-naphthalimide-3-formaldehyde-(2-pyridine)hydrazone and an application in realizing fluorescence imagingof Fe3+ in cells. The N-butyl-4-hydroxyl-1,8-naphthalimide-3-formaldehyde-(2-pyridine)hydrazone and the application have the beneficial effects that the synthesis method of the N-butyl-4-hydroxyl-1,8-naphthalimide-3-formaldehyde-(2-pyridine)hydrazone is simple; the N-butyl-4-hydroxyl-1,8-naphthalimide-3-formaldehyde-(2-pyridine)hydrazone is used for detection of Fe3+ as a fluorescence probe, the selection for Fe3+ is good, the interference of other coexisting positive ions can be avoided, the detection sensitivity is high, simultaneously the color is changed into light yellow from pink, the naked-eye recognition can be realized, the operation method is simple, the cost is low, and the fast and convenient effects are achieved; fluorescence imaging of Fe3+ can be realized in the cells, so that the potential application value is achieved in the aspect of sensor development.

The invention provides an aflatoxin B1 aptamer, a DNA sensor, a kit and application. The aflatoxin B1 aptamer has the nucleotide sequence as follows: 5'-GTTmmmmmmmTGTTGTCTCTCT GTGTCTnnnnnnnTTCGCTAGGCCCACA-3', each m and n independently are A or T or C or G, and the m chain segment and the n chain segment achieve complementary pairing. According to the aflatoxin B1 aptamer, the DNA sensor, the kit and the application, no large instrument equipment is needed when the aflatoxin B1 is measured, and therefore the cost is low; a constant-temperature reaction is achieved, qualitative detection of naked eyes can be achieved, and quantitative detection can also be achieved; in addition, no fluorochrome is needed, and therefore the cost is greatly reduced.

The invention relates to the field of detection of pesticide residues and provides a detection method for screening 708 pesticide residues in leaf vegetables. In the GC-Q-TOF/MS first-class mode, a fragment ion full-scanning mass spectrum on the specified conditions of a pesticide standard, the chemical formula, the retention time, the fragment ion accurate mass number and the ion abundance ratio of pesticide are obtained, guided into PCDL software and related to corresponding pesticide information to form a database, and full-spectrum data of samples to be tested is determined on the identical set condition to obtain full-spectrum data. A sample measuring result is retrieved through a precise mass database, the detected pesticide meeting the identification basis is accurately identified, and pesticide residue varieties in leaf vegetables are obtained. The detection method built by applying the GC-Q-TOF/MS high resolution mass spectrum is used for detecting pesticide residues in leaf vegetables, specified pretreatment is preferentially combined, and the advantages that detection is rapid and high in throughput, precision and reliability are achieved. No standard is used as a contrast, and meanwhile 708 pesticides in the leaf vegetables are qualitatively detected and confirmed.

The invention relates to a medical radiographic image phantom measurement standard device and a phantom detection method, and provides an X-ray scanning three-dimensional tomography imaging device foracquiring the internal quality state and structural information of a medical radiographic image phantom. According to the technical scheme, the device comprises a platform, a movement mechanism, a ray tube and a detector, the platform comprises a base and two stand columns oppositely installed on the base, a workpiece movement platform is connected to the base in a sliding mode, a workpiece rotary table is connected to the workpiece movement platform in a rotating mode, and the ray tube and the detector are connected to the opposite faces of the two stand columns in an up-down sliding mode respectively. According to the invention, qualitative detection of material density is realized, and measurement of material CT value material density by industrial CT is realized.

The invention belongs to the technical field of fluorescence spectrum detection and analysis, and particularly relates to a visual organic molecular fluorescence probe on the basis of cyanine and a method for preparing the visual organic molecular fluorescence probe. The visual organic molecular fluorescence probe comprises a cyanine fluorophore and benzothiazole groups. The method includes stepsof (1), dropwise adding phosphoryl chloride into N, N'-dimethylformamide to obtain mixtures, then adding the mixtures into bromoacetic acid and carrying out reaction to obtain products II; (2), carrying out reaction on 2, 3, 3'-trimethyl indole and ethyl iodide to obtain products III; (3), dropwise adding piperidine into the products II and the products III and carrying out reaction to obtain products IV; (4), carrying out reaction on the products IV and aminothiophenol at the room temperature to obtain the visual organic molecular fluorescence probe. The visual organic molecular fluorescenceprobe and the method have the advantages that obvious color change of the visual organic molecular fluorescence probe can be seen in natural light or ultraviolet lamps, linear relations between the fluorescence intensity and peroxynitrite negative ions are established, and accordingly the peroxynitrite negative ions can be qualitatively and quantitatively detected; the visual organic molecular fluorescence probe is good in selectivity and high in sensitivity, detection methods are easy to implement, and obvious effects can be realized.

The invention provides an LC-Q-TOF/MS detection technique for 544 pesticide residues in solanaceous fruit vegetables. In a TOF/MS mode, LC-Q-TOF/MS measures the retention time of each pesticide standard substance under specified chromatographic and mass spectrometric conditions, an ionization form and a chemical formula of each compound under an ESI source are determined, the exact mass number for parent ions of each compound is obtained, and a TOF/MS database is formed. In a Q-TOF/MS mode, fragment ion mass spectra of each pesticide standard substance in 3-5 different collision energies are collected respectively, the collected information is introduced into PCDL software, and a Q-TOF/MS database is formed. Through comparison of the retention time and first-level mass spectrometry and second-level mass spectrometry information of solanaceous fruit vegetable samples, whether the samples contain the pesticide residues is determined, compounds having higher first-level score are subjected to second-level confirmation, and if the second-level score is higher, related pesticide residues are confirmed to be detected out. The method has the advantages of high speed, high throughput, high precision, high reliability and the like, and can accurately screen the pesticides in the solanaceous fruit vegetables.

The invention relates to an ordered nanostructure array-based LSPR (localized surface plasma resonance) sensing device. The ordered nanostructure array-based LSPR (localized surface plasma resonance)sensing device includes a light source unit, a sensing unit, a detection unit and a display unit; the sensing unit is arranged between the light source unit and the detection unit; the detection unitis electrically connected with the display unit; the light source unit is used for providing light signals; the sensing unit is a semi-closed cell culture dish of which the inner surface integrates anordered nanostructure array; the light signals emitted by the light source unit can pass through the sensing unit to reach the detection unit; the detection unit is used for converting the light signals into electric signals; and the display unit is used for outputting a detection result. According to the ordered nanostructure array-based LSPR (localized surface plasma resonance) sensing device disclosed by the invention, cell-substrate contact area signals are converted into LSPR spectral intensity signals in real time, so that the in-situ real-time monitoring of a cell adhesion and spreading whole process is realized; and the cell-substrate contact area signals are linearly related to the LSPR spectral intensity signals, so that the semi-quantitative monitoring of the cell adhesion andspreading whole process is realized.

The invention belongs to the field of flow cytometry technology, and specifically relates to an intracellular protein detection method by the use of flow cytometry. The method comprises the following steps of: connecting an amino or carboxy functional group on the surface of 100-120nm silica nanoparticle with an amino group of an antibody against the intracellular protein to be detected by the use of covalent bonds, incubating the silica nanoparticle together with intracellular proteins obtained by ultrasonic and other methods, adding another differently sourced antibody which targeted the same intracellular protein to be detected and carrying out incubating, then incubating with a fluorescein labeled second antibody to form a silica nanoparticle-antibody-intracellular protein-antibody-fluorescein labeled second antibody compound, and finally carrying out qualitative detection on the compound by using flow cytometry. According to the invention, after binding to the antibody against the intracellular protein to be detected, the silica nanoparticle has initiative targeting ability and can target various different intracellular proteins.

The invention relates to the field of pesticide residue detection and provides a detection method for screening 708 pesticide residues in legume vegetables. The method includes the steps of: a) under a first-stage mode of GC-Q-TOF/MS, collecting a fragment ion full-scanning mass spectrogram of pesticide reference substances under certain conditions to obtain a chemical formula, retention time, accurate mass numbers of fragment ions, and ion abundance ratio of the pesticide; b) introducing the data to PCDL software, correlating the data with corresponding pesticide information to obtain a database; c) under the same preset conditions, detecting the full-spectrum data of the to-be-detected samples; and d) performing accurate mass database retrieval to the sample detection results, and accurately identifying the detected pesticide satisfying identification basis to obtain the types of the pesticide residues in the legume vegetables. By means of the GC-Q-TOF/MS high-resolution mass spectrum, the detection method can detect the pesticide residues in the legume vegetables, preferably, with combination of special pre-treatments, so that the method is quick, high-throughput, high-precision and high-reliability. The method is free of a reference substance and can be used for qualitatively detecting and identifying the 708 pesticides in legume vegetables.

The invention relates to the field of pesticide residue detection, and provides a detection method for screening 708 kinds of pesticide residues in melon fruit. The detection method comprises the following steps: acquiring a fragment ion full-scan mass spectrogram under specified conditions of pesticide standards in a GC-Q-TOF/MS level-one mode to obtain chemical molecular formulae, retention time, precise fragment ion mass numbers and ion abundance ratios of pesticides; importing the chemical molecular formulae, the retention time, the precise fragment ion mass numbers and the ion abundance ratios of the pesticides into PCDL software; correlating the chemical molecular formulae, the retention time, the precise fragment ion mass numbers and the ion abundance ratios of the pesticides with corresponding pesticide information to form a database; measuring full spectrum data of a sample to be detected under the same set conditions to obtain the full spectrum data; retrieving a detection result of the sample in a precise mass database to accurately authenticate the detected pesticides meeting an authentication basis to obtain pesticide residue varieties of the melon fruit. According to the detection method for screening 708 kinds of pesticide residues in the melon fruit, the pesticide residues in the melon fruit are detected by the detection method established by utilizing GC-Q-TOF/MS high resolution mass spectrum; preferably, a specified pre-treatment is combined; the detection method has the characteristics of quickness, high throughput, high precision and high reliability. A standard substance is not required for contrasting, and 708 pesticides in the melon fruit are detected and confirmed qualitatively.

The invention discloses a preparation method of a non-conjugated fluorescent polymer and application thereof in picric acid detection. The preparation method of the non-conjugated fluorescent polymercomprises the following steps: by taking polyethyleneimine and citric acid as raw materials and taking deionized water as a solvent, obtaining a product solution after heating and stirring reaction, and carrying out dialysis, concentration and drying to prepare and obtain the non-conjugated fluorescent polymer. When trace picric acid is added into a non-conjugated fluorescent polymer water solution, due to electron transfer and inner filtering effect between picric acid and the non-conjugated fluorescent polymer, fluorescence quenching of the non-conjugated fluorescent polymer is caused, and the quenching degree and the concentration of picric acid are of a linear reaction within a certain range. Therefore, a method for detecting picric acid is established, the method is simple in operation, high in sensitivity and high in selectivity, and the picric acid can be detected quickly in real time.

The present invention establishes a LC-Q-TOF/MS detection technology for 544 kinds of pesticide residues in melon vegetables. LC-Q-TOF/MS in TOF/MS mode, respectively measure the retention time of each pesticide standard under the specified chromatographic mass spectrometry conditions, determine the ionization form and chemical formula of the compound under the ESI source, and obtain the parent ion of each compound Accurate mass number of the TOF/MS database. In the Q-TOF/MS mode, the fragment ion mass spectra of each pesticide standard were collected under 3-5 different collision energies, and the collected information was imported into the PCDL software to form a Q-TOF/MS database. By comparing the retention time, primary mass spectrometry and secondary mass spectrometry information in melon and vegetable samples, determine whether the sample contains pesticide residues, and perform secondary confirmation on compounds with higher primary scores. If the secondary score is higher, it is confirmed Relevant pesticide residues were detected. The invention has the advantages of rapidity, high throughput, high precision, high reliability and the like, and can accurately screen pesticides in melons and vegetables.

The invention discloses a method for detecting lactoperoxidase in raw milk using test paper. The method includes the following steps: preparing a reaction reagent; conducting test paper reaction; establishing detection standards; detecting lactoperoxidase in a milk sample. The method provided by the invention has the advantages that the detection speed is high, and a quantitative detection of lactoperoxidase can be finished in a few seconds at the soonest; the content of lactoperoxidase in raw milk can be quantitatively detected in 3 minutes; the method is suitable for the online detection of lactoperoxidase; the detection cost is low; the detection result is accurate, and the lowest detected concentration is 1-2 mg/L; the requirements for the environment are low; the detection operations are simple and convenient.

The invention provides a copper ion detection method based on methanobactin functional gold nanoparticles, which relates to a method of detecting trace copper ions in a sample. The method comprises the following steps: firstly, methanobactin is used to catalyze hydrogen tetrachloroaurate hydrate for in-situ synthesis of a methanobactin functional gold nanoparticle solution; a pretreated control sample and a to-be-detected sample are then added to the methanobactin functional gold nanoparticle aqueous solution, after standing for a period of time, the color of the to-be-detected sample changesinto blue-violet while the color of the control sample is unchanged, and thus, the detected sample is proved to contain copper ions. The method uses the specific combination between methanobactin andcopper to realize copper ion detection, the specificity is strong, the detection result can be distinguished by naked eyes, no complicated operation step and no large instrument is needed, and quick copper ion detection can be realized.

The invention provides a quartz crystal microbalance biosensing method based on a mass load reduction method. A biomolecular probe (such as an antibody and a single-stranded DNA) is modified on a quartz crystal, a detection solution (such as pathogenic bacteria, DNA molecules to be detected and the like) is added to the surface of a sensor, a frequency spectrum scanning system is developed by adopting a direct digital synthesis (DDS) technology, and scanning excitation is carried out on a quartz crystal oscillator according to a preset frequency and sine wave amplitude; and a quartz crystal frequency signal acquisition and analysis system is constructed based on a superheterodyne receiving orthogonal phase-sensitive detection technology, and a QCM response signal amplitude-frequency and phase-frequency characteristic curve is drawn. Quartz crystal microbalance biosensing by a mass load reduction method is realized through a QCM response signal spectrum. According to the method, a detection signal is obtained in situ in the detection liquid, and a quartz crystal microbalance is directly adopted for biosensing detection, so that the method has important application value for medicalinstant detection and on-site rapid detection.