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Immunochromatography detection card for detecting fluorescent microspheres of ketamine and methyl amphetamine and preparation method thereof

A technology of methamphetamine and fluorescent microspheres, which is applied in the direction of measuring devices, analytical materials, instruments, etc., can solve the problems of multi-dilution of samples, limited sensitivity, and many interfering components, and achieves low price, high sensitivity, and increased The effect of fluorescence lifetime

Active Publication Date: 2009-10-14
江西中德生物工程股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, no matter what kind of principle is used for rapid reagents used in China, due to their sensitivity limitations, the sample cannot be diluted multiple times, so that there are many interfering components in the sample, resulting in false negatives or false positives in the actual sample detection process. Phenomenon

Method used

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  • Immunochromatography detection card for detecting fluorescent microspheres of ketamine and methyl amphetamine and preparation method thereof
  • Immunochromatography detection card for detecting fluorescent microspheres of ketamine and methyl amphetamine and preparation method thereof
  • Immunochromatography detection card for detecting fluorescent microspheres of ketamine and methyl amphetamine and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] 1. Preparation of fluorescent microsphere markers (EDC method):

[0046] Preparation of fluorescent microsphere-labeled anti-ketamine or anti-methamphetamine antibodies: Take 1 mg of fluorescent microspheres coated with phenanthroline biruthenium organic dye and centrifuge at 1000×g for 10 min, collect the precipitate, and buffer with 0.01M borate pH4.8 solution to adjust the concentration of microspheres to OD 450 =0.2, then add 90μl 50mg / ml EDC, 150μl 5mg / ml NHS, shake and mix well, incubate at room temperature for 20min, centrifuge at 1000×g for 5min, dissolve the precipitate with 0.01M borate buffer solution of pH4.8, and adjust micro Ball concentration is OD 450 is 0.5. at 0.1ml OD 450 Add 1 μg of anti-ketamine or anti-methamphetamine antibody to the fluorescent microspheres with a pH of 0.5, mix well, stir and react at room temperature for 3 h, wash and centrifuge with ultrapure water for 3 times, and wash with 0.01M PBS with pH 7.2 (containing 5 % sucrose and...

Embodiment 2

[0059] The preparation method of the present embodiment is basically the same as that of Example 1, the difference is:

[0060] The carrier protein is ovalbumin; the labeled carrier fluorescent microspheres are quantum dot-silica shell-core dual-structure microspheres.

[0061] The qualitative detection of ketamine and methamphetamine with the above-mentioned fluorescent microsphere immunochromatographic detection card comprises the following steps:

[0062] (1) Lay the test card flat, after the blood sample to be tested is equilibrated to room temperature, add 90 μl of sample into each of the two sample holes of the test card, react at room temperature for 15 minutes, and put the test card into the detection window;

[0063] (2) The fluorescent microspheres trapped in the detection area and the quality control area emit strong fluorescence under the excitation of the best excitation light source; when observed in the observation window, there is a fluorescent band in the dete...

Embodiment 3

[0065] The preparation method of this example is basically the same as that of Example 1, with the difference that: the labeling carrier fluorescent microspheres are isothiocyanatofluorescein-silicon dioxide shell-core dual-structure microspheres.

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Abstract

The invention discloses an immunochromatography detection card for detecting fluorescent microspheres of ketamine and methyl amphetamine and a preparation method thereof. The detection card is a double-card type, wherein one card is used for detecting the ketamine and the other is used for detecting the methyl amphetamine so as to realize the simultaneous detection of the ketamine and the methyl amphetamine. The detection card sequentially comprises a filter paper, a sample pad, a glass fiber film, a pyroxylin film and a drinking paper; antibodies marked by fluorescent microspheres are sprayedon the glass fiber film; and a detection zone and a quality-control zone are respectively fixed on the pyroxylin film. The invention adopts a nucleocapsid double-structure shining nano-particle compounded by silicon dioxide and fluorescent substance as a mark, and also adopts immunochromatography technology to realize the quick immunoassay of the ketamine and the methyl amphetamine. When in detection, an optimal excitation light source of the fluorescent microsphere is adopted to carry out excitation, and then whether the fluorescent substance exists on detection line can be detected by nakedeye after excited fluorescence passes through a spectral filter device. The detection card has the advantages of high sensitivity, quick detection performance, convenient operation and economical andpractical property.

Description

technical field [0001] The invention belongs to the field of anti-drug detection, and in particular relates to a detection card for qualitative detection of ketamine and methamphetamine using fluorescent microsphere immunochromatography technology and a preparation method thereof. Background technique [0002] Ketamine, commonly known as "K powder", is a derivative of phencyclidine, an N-methyl-D-aspartate receptor antagonist, and a non-barbiturate anesthetic. In recent years, with the popularity of "prom drugs" related to special "social" and "sexual environments" such as stimulants, γ-hydroxybutyric acid and marijuana in European and American countries, the problem of K powder abuse has become increasingly serious. The abuse of K fans has begun to appear in our country, and it is becoming more and more serious. Relevant data show that 70mg of K powder can cause poisoning, a large dose of 200mg can produce hallucinations, such as entering a mild and magical world, and 500m...

Claims

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Application Information

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IPC IPC(8): G01N33/566G01N33/558G01N33/533
Inventor 陈雪岚赖卫华熊勇华魏华李林
Owner 江西中德生物工程股份有限公司
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