Method for preparing flexible SERS (Surface Enhanced Raman Scattering) substrate and application thereof in specific detection of PAT

A specific and flexible technology, applied in measurement devices, instruments, material analysis by optical means, etc., can solve the problems of low accuracy or sensitivity, difficult to detect quickly and accurately, and achieve high accuracy, low cost, and selectivity. strong effect

Active Publication Date: 2018-05-04
HUAZHONG AGRI UNIV +1
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  • Claims
  • Application Information

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Problems solved by technology

[0009] At present, PAT in food is mainly detected by microbial method, thin-layer chromatography, gas chromatography, high-performance liquid chromatography, and immune method. However, the above-mentioned methods have defects of low accuracy or sensitivity in detection, and it is difficult to realize Fast and accurate detection

Method used

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  • Method for preparing flexible SERS (Surface Enhanced Raman Scattering) substrate and application thereof in specific detection of PAT
  • Method for preparing flexible SERS (Surface Enhanced Raman Scattering) substrate and application thereof in specific detection of PAT
  • Method for preparing flexible SERS (Surface Enhanced Raman Scattering) substrate and application thereof in specific detection of PAT

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preparation example Construction

[0033] An embodiment of the present invention provides a method for preparing a flexible SERS substrate, comprising the following steps:

[0034] S1. Anodize aluminum (purity 99.999%) to obtain an AAO (anodized aluminum oxide) template with regular porous structure:

[0035] The aluminum sheet is used as the anode, and the electrolyte with a concentration of 0.1-0.5M is used (the electrolyte can be selected from sulfuric acid, oxalic acid or phosphoric acid). It is oxidized for 2 to 6 hours under the conditions of high temperature, and then put into chromic acid with a temperature of 50 to 70 ° C, a concentration of 1 to 3 wt%, and a concentration of 5 to 8% H. 3 PO4 The oxide layer was removed from the mixed solution to obtain a preliminary porous structure template. In this embodiment, 0.1-0.5M oxalic acid solution is used as the electrolyte. In actual operation, the concentration and type of the electrolyte can be selected according to actual needs. The above electrolyte c...

Embodiment 1

[0060] S101. Cut the high-purity aluminum sheet into 2×2cm 2 After being fixed on the glass sheet, it was used as the anode, and the oxalic acid solution with the concentration of 0.3M was used as the electrolyte, and the oxidation voltage was 40V, the oxidation current was 15mA, and the temperature was 0 ℃. is 60°C and contains 1.8wt% CrO 3 and a concentration of 6% H 3 PO 4 The oxide layer was removed by soaking in the mixed solution for 30 min, and a preliminary porous structure template was obtained.

[0061] S102, using the preliminary porous structure template as the anode, put into the oxalic acid solution with a concentration of 0.3M, oxidize for 0.5h under the conditions of an oxidation voltage of 40V, an oxidation current of 15mA, and a temperature of 0°C, the temperature is 30°C, and the concentration 5wt% H 3 PO 4 Pore ​​expansion treatment was carried out in the solution for 30 min to obtain porous anodic alumina (AAO).

[0062] S103, put the AAO into the pl...

Embodiment 2

[0069] S201. Cut the high-purity aluminum sheet into 2×2cm 2 After being fixed on a glass sheet, it was used as the anode, and the oxalic acid solution with a concentration of 0.1M was used as the electrolyte, and the oxidation voltage was 30V, the oxidation current was 20mA, and the temperature was -5 ℃. The temperature is 40°C and the concentration of CrO is 3wt% 3 and a concentration of 6% H 3 PO 4 The oxide layer was removed by soaking in the mixed solution for 10 min to obtain a preliminary porous structure template.

[0070] S202, using the preliminary porous structure template as the anode, put into the oxalic acid solution with a concentration of 0.1M, oxidize for 20min under the conditions that the oxidation voltage is 30V, the oxidation current is 20mA, and the temperature is -5°C, the temperature is 20°C, and the concentration 3wt% H 3 PO 4 Pore ​​expansion treatment was performed in the solution for 10 min to obtain porous anodic alumina (AAO).

[0071] S203,...

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Abstract

The invention discloses a method for preparing a flexible SERS (Surface Enhanced Raman Scattering) substrate and application thereof in specific detection of PAT and relates to the fields of nanometermaterial science and chemical test analysis. The method comprises the following steps: preparing anodic aluminum oxide AAO with a regular porous structure, coating the surface of the AAO with a softening agent to obtain a flexible composite film, and depositing a precious metal X on the surface of the flexible composite film, thereby obtaining the flexible SERS substrate. The flexible SERS substrate subjected to specific detection comprises a flexible composite film, wherein precious metal nanoparticles are distributed on the surface of the flexible composite film; and template molecules arebound onto the surface of the flexible composite film. The flexible SERS substrate disclosed by the invention has excellent selectivity, the application of the flexible SERS substrate in specific detection of PAT is high in accuracy and sensitivity, and the detection speed is high.

Description

technical field [0001] The invention relates to the fields of nanomaterial technology and chemical testing and analysis, in particular to a preparation method of a flexible SERS substrate and its use for specific detection of PAT. Background technique [0002] Since its discovery in the mid-1970s, surface-enhanced Raman spectroscopy (SERS) technology has been widely used in biomedicine, surface science, and other fields due to its unique advantages such as narrow spectral band, resistance to photobleaching, in situ, non-destructiveness, and the ability to provide fingerprint information. , molecular recognition, trace detection and many other fields have been widely used. [0003] When SERS is used for detection, the analyte is usually adsorbed on the SERS substrate. The electromagnetic enhancement of the analyte is caused by the excitation of the surface localized plasmon. At the same time, the atomic clusters on the SERS substrate and the molecules adsorbed on it constitut...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/65
CPCG01N21/658
Inventor 韩鹤友殷文敏李晋杰严恒吴龙吕志成王盼
Owner HUAZHONG AGRI UNIV
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