13 results about "Surface binding" patented technology

The invention relates to a monomolecular in-situ recognition and detection method based on intermolecular hydrogen bonds, and belongs to the technical field of monomolecular detection.The method comprises the steps that a gold nanoelectrode is prepared through an electrochemical corrosion method, then a bridging molecule solution with sulfydryl is combined with the surface of the gold nanoelectrode, meanwhile, gold nanoparticles are mixed with the bridging molecule solution, and the gold nanoparticles are obtained; the method comprises the following steps: modifying a gold nano-electrode, placing the modified gold nano-electrode in a modified gold nano-particle solution, adding a target molecule solution into the gold nano-particle solution, forming molecular knots by virtue of an electrostatic adsorption effect between gold nano-particles and the gold nano-electrode, dynamically adjusting the molecular knots by virtue of electrochemical regulation, and finally monitoring in real time by virtue of an SERS (Surface Enhanced Raman Scattering) signal. And obtaining identification information of the target molecule. Therefore, the method has the advantages of high identification precision, high sensitivity and real-time dynamic monitoring.

This disclosure relates to a sample analyzer, a sample analysis method, and a reagent kit. The controller in the analyzer is configured to perform the following operations: control a reagent dispensing mechanism to draw a first reagent and a sample to be tested into a reaction vessel to obtain a first reaction system and react for a first predetermined time, wherein the first reagent includes a first carrier with a first ligand bound to its surface capable of binding to the analyte; control the reagent dispensing mechanism to further draw a second reagent into a reaction vessel to obtain a second reaction system and react for a second predetermined time, wherein the second reagent includes a second ligand capable of binding to the analyte; control a photometric component to acquire at least two optical signals and thereby obtain a first reaction analysis value of the first reaction system and a second reaction analysis value of the second reaction system; and obtain an analytical result for the analyte based on the two reaction analysis values.

A method for ion mapping of a sample, which has the following features: Application of a liquid sample to a metallic target surface, Separation of components of the liquid sample on the metallic target surface according to one or more physicochemical properties in a first spatial dimension and in a second spatial dimension and Ionizing and mass-analyzing multiple separated areas of the separated sample to generate an ion map of at least a portion of the sample separated on the target surface, wherein the components of the sample are applied to the target surface and separated by mechanical, hydrodynamic and / or aerodynamic means, and wherein the one or more physicochemical properties include one or more from the group comprising surface binding, surface affinity, solubility, binding affinity and polarity.

Embodiments of this application provide a metadynamics-based target discovery method and a related apparatus, and are applied to the fields of biology, medicine, and drug design. The method includes: performing dynamics simulation on a ligand structure and a receptor structure, to enable the ligand structure to traverse a surface of the receptor structure during the dynamics simulation; determining target information of binding sites of the ligand structure on the surface of the receptor structure; and outputting pocket information of the receptor structure based on sorting of the target information of the binding sites. In this process, a plurality of binding sites can be obtained through one traversal. In this way, a receptor conformation search range can be wider, a calculation amount is reduced, and binding site discovery efficiency is improved.

The present invention provides a boron affinity chromatography medium and a preparation method thereof. The boron affinity chromatography medium comprises a silica gel coated with poly(styrene-divinylbenzene) as a matrix, 3-acrylamidophenylboronic acid is grafted onto the matrix, and then modified with 6-aminopyridine boronic acid to obtain a boron affinity chromatography medium. The present invention can increase the surface binding sites of the matrix and improve the adsorption capacity of the chromatography medium by coating the silica gel surface with poly(styrene-divinylbenzene). Meanwhile, 6-aminopyridine boronic acid is used for modification to increase the adsorption sites of the chromatography medium, thereby further improving the adsorption capacity.

The invention discloses a method for preparing a highly selective core-shell magnetic nanoparticle for antibody separation, comprising the following steps: preparing ferroferric oxide / silicon oxide nanoparticles with a core-shell structure, performing amine functionalization treatment on the nanoparticles, and finally coupling IgG2a on the surface thereof; dispersing the IgG2a-coupled magnetic nanoparticles in a PBS buffer, adding tetraethyl silicate to stir the reaction, adding a mixed solution of N-propyltriethoxysilane, benzyltriethoxysilane, and (3-aminopropyl)-triethoxysilane, reacting, and after the reaction is completed, dripping a mixed solution of methanol, PEG-4, and NHS to continue stirring, washing the precipitate with a PBS buffer, and finally adding the precipitate to a mixed solution of a sodium hydroxide solution and a polyethylene glycol octylphenyl ether solution for treatment, obtaining magnetic nanoparticles, and redispersing the nanoparticles in a PBS buffer for standby use. The core-shell magnetic nanoparticles provided by the invention have many surface binding sites and high selectivity, and can effectively purify monoclonal shells.

Compositions and methods are provided for the cell-mediated targeted killing of diseased cells based on the presence of an intracellular antigen, rather than a surface-bound marker. The targeting cells are modified to express a cytotoxic protein that is delivered into a targeted cell, and after delivery is selectively activated by the presence of a cytoplasmic protein of interest. In one embodiment of the invention, the cytotoxic molecule is a Granzyme B (GrB) polypeptide. In the compositions of the invention, GrB is modified to render its cytotoxic enzymatic functions inactive, until the presence of an intracellular antigen unlocks the GrB molecule to enable enzymatic activities.

According to various aspects, the present invention provides a method of delivering a plurality of sample solutions to a sensor surface (102) for analysis. It may additionally or alternatively provide systems and methods for enabling an improved study of analyte behavior. A sensor (102) may be used, for example, to determine kinetic binding properties of an analyte, such as a protein, to a surface binding ligand. In one aspect, a method includes: a) introducing (902) a first sample solution (502) into a first channel (506), the first channel (506) configured to deliver the sample solution to a sensor surface (102); b) introducing (904) a second sample solution (504) into the first channel (506); c) flowing (906) the first sample solution and the second sample solution through the first channel (506) and over the sensor surface (102); and d) detecting (912) at the sensor surface (102) the presence or absence of binding of the analyte from at least one of the sample solutions (502, 504) wherein no running buffer solution passes over the sensor surface (102) throughout steps a)-d).

An atomic layer deposition (ALD) method may include forming a chemisorbed, surface-bound metal-ligand species using a metal M (II) primary precursor of formula (I):In formula (I), M is Sn or Ge or Pb; L is a ligand displaying ALD reactivity for a secondary precursor; and R1, R2, and R3 are each independently selected from: H or a linear or branched alkyl groups. At least one of R1, R2 and R3 is a linear or branched alkyl group.

The invention belongs to the field of electrochemical biosensors, and particularly relates to a protein molecular marking method for constructing a high-performance electrochemical sensor. Organic ligands on the surfaces of sulfide colloidal quantum dots are replaced with water-soluble ligands through liquid-phase ligand replacement, then the water-soluble ligands are mixed with carboxyl activating agents to activate carboxyl of the water-soluble ligands, then the water-soluble ligands are uniformly mixed with protein molecules, and the protein molecules are effectively marked on the quantum dots. A working electrode of a planar three-electrode is coated with the quantum dots with the surfaces combined with the water-soluble ligands and marked with the protein molecules through a one-step method, and after drying, the electrochemical biosensor which is wide in detection range, high in response sensitivity and high in anti-interference performance is prepared.

Methods of in-situ next generation sequencing (NGS) target enrichment using a NGS library with truncated surface binding regions are described herein.

The present invention relates to a sample analyzer, a sample analysis method, and a reagent kit. A controller in the analyzer is configured to perform the following operations: controlling a reagent dispensing mechanism to suction a first reagent and a sample under test and provide same into a reaction cup to obtain a first reaction system for reaction for a first predetermine time, wherein the first reagent comprises a first carrier having a first ligand bound to the surface thereof, wherein the first ligand can bind to an analyte; controlling the reagent dispensing mechanism to further suction a second reagent and provide same into the reaction cup to obtain a second reaction system for reaction for a second predetermined time, wherein the second reagent comprises a second ligand that can bind to the analyte; controlling an optical measurement component to acquire at least two optical signals, so as to obtain a first reaction analysis value of the first reaction system and a second reaction analysis value of the second reaction system; and obtaining an analysis result of the analyte on the basis of the two reaction analysis values.

Described herein are dual affinity probes that include an organic or inorganic surface-binding peptide and a target-specific capture element that can bind to various targets, such as pathogens. Further described herein are uses of the dual affinity probes and kits for determining the presence and / or amount of targets in a sample. In certain embodiments, the dual affinity probes are specific for SARS-CoV-2 (spike or nucleocapsid) proteins and can be used to determine whether a subject is infected with SARS-CoV-2.