Aflatoxin B1 aptamer, DNA sensor, kit and application

A technology of aflatoxin and nucleic acid aptamer, which is applied in the direction of recombinant DNA technology, DNA/RNA fragments, biochemical equipment and methods, etc., can solve the problems of cumbersome operation and high cost, and achieve simple operation, low cost, and portability Effect
CN105505940AActive Publication Date: 2016-04-20SHENZHEN KIVITA INNOVATIVE DRUG INST
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
SHENZHEN KIVITA INNOVATIVE DRUG INST
Publication Date
2016-04-20

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Abstract

The invention provides an aflatoxin B1 aptamer, a DNA sensor, a kit and application. The aflatoxin B1 aptamer has the nucleotide sequence as follows: 5'-GTTmmmmmmmTGTTGTCTCTCT GTGTCTnnnnnnnTTCGCTAGGCCCACA-3', each m and n independently are A or T or C or G, and the m chain segment and the n chain segment achieve complementary pairing. According to the aflatoxin B1 aptamer, the DNA sensor, the kit and the application, no large instrument equipment is needed when the aflatoxin B1 is measured, and therefore the cost is low; a constant-temperature reaction is achieved, qualitative detection of naked eyes can be achieved, and quantitative detection can also be achieved; in addition, no fluorochrome is needed, and therefore the cost is greatly reduced.
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Description

technical field

[0001] The present invention relates to the field of biological detection, and more specifically, relates to an aflatoxin B1 nucleic acid aptamer, a DNA sensor comprising the nucleic acid aptamer, a test kit comprising the DNA sensor, and the DNA sensor and the test kit. Background technique

[0002] Aflatoxins are secondary metabolites mainly produced by Aspergillus flavus and Aspergillus parasiticus, and the probability of aflatoxins appearing in food and feed in hot and humid areas is the highest. They exist in soil, animals, plants, and various nuts, especially grain and oil products such as peanuts, corn, rice, soybeans, and wheat. They are the most toxic mycotoxins and are extremely harmful to human health. In natural food, aflatoxin B1 is the most common and most harmful.

[0003] Currently available aflatoxin detection methods mainly include:

[0004] (1) TLC

[0005] Thin layer chromatography is the most widely used separation technique in the stu...

Claims

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