COVID-19 nucleic acid testing primer probe composition, COVID-19 nucleic acid testing kit and COVID-19 nucleic acid testing method

Abstract

The invention relates to the field of the biological technology and the medical examination technology, and provides a COVID-19 nucleic acid testing primer probe composition, a COVID-19 nucleic acid testing kit and a COVID-19 nucleic acid testing method for solving the problems of low accuracy, poor specificity and low sensitivity of a traditional virus nucleic acid testing method. The COVID-19 nucleic acid testing primer probe composition comprises a first primer probe group, a second primer probe group and a third primer probe group, wherein the first primer probe group comprises a forward primer ORF1ab-F, a probe ORF1ab-P and a reverse primer ORF1ab-R; the second primer probe group comprises a forward primer N-F, a probe N-P and a reverse primer N-R; and the third primer probe group comprises a forward primer Rpp30-F, a probe Rpp30-P and a reverse primer Rpp30-R. According to the primer probe composition disclosed by the invention, through artful design of an amplification primer pair and a detection probe, mutual interference between a plurality of primer pairs and corresponding detection probes is avoided. The kit has a simple structure, an interior label is used for monitoring a collection, transportation and extraction process of a sample to be detected, and the false negative of a detection result is avoided.

Description

technical field [0001] The invention relates to the technical fields of biotechnology and medical testing, in particular to a novel coronavirus nucleic acid detection primer probe composition, kit and method. Background technique [0002] The new coronavirus (COVID-19) is a positive-sense single-stranded RNA coronavirus with an envelope. The length of each genome of this virus is about 30,000 nucleotides, and it belongs to the coronavirus lineage B (BetacoronavirusLineage B , Sarbecovirus). Similar to SARS-CoV, COVID-19 can be bound by human ACE2 as a receptor, enter the lungs through respiratory epithelial cells for replication, and the main target is the same T lymphocytes as SARS-CoV. It is mainly transmitted through unprotected close contact with infected people and droplets, and may also be transmitted through aerosols. The basic clinical symptoms are fever, dry cough, and fatigue. A small number of patients have symptoms such as nasal congestion, runny nose, sore th...

AI Technical Summary

Problems solved by technology

[0007] In order to overcome the problems of low accuracy, poor specificity and low sensitivity of traditional virus nucleic acid detection methods, the present invention provides a novel coronavirus nucleic acid detection primer probe composition with high accuracy, good specificity and high sensitivity, which avoids multiple The mutual interference between each primer pair and the corresponding detection probe

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