17 results about "Nucleic Acid Testing" patented technology

Provided are a portable nucleic acid detection device, system and method. The device comprises a main body (1). The main body (1) is provided with a mounting cavity for mounting a bearing module (11). The bearing module (11) is configured to accommodate a reaction tube (2). The reaction tube (2) is configured to accommodate a to-be-detected sample (21). The bearing module (11) is connected to a heating module (12). The heating module (12) is configured to regulate a temperature of the bearing module (11), so as to regulate a temperature of the reaction tube (2). The main body (1) is connected to a light-emitting module (13). The light-emitting module (13) is arranged at a bottom of the bearing module (11). The bearing module (11) is provided with a light-transmission hole (111) facing the light-emitting module (13), so that emitted light of the light-emitting module (13) is irradiated on the reaction tube (2) by means of the light-transmission hole (111). The main body (1) is further provided with an observation window (14). A first side of the bearing module (11) is provided with a first observation hole (112). The first observation hole (112) is configured for observation of the reaction tube (2). When the bearing module (11) is mounted in the mounting cavity, the observation window (14) is configured for observation of the reaction tube (2) by means of the first observation hole (112).

1. Name of the product in this design: Consumables Box. 2. Purpose of this design: To place a tray to support the TIP head, PCR tube and PCR tube cap for nucleic acid testing. 3. The key design feature of this product is its shape. 4. The image or photograph that best illustrates the design's key points: a 3D model.

The present application relates to the technical field of nucleic acid testing. Disclosed is a nucleic acid integrated testing apparatus based on a flexible structure and a push-pull structure, the apparatus comprising a testing main body, an upper cover, a bottom housing, and a squeezing block provided between the upper cover and the bottom housing; the testing main body is provided in an accommodating cavity between the upper cover and the bottom housing; the testing main body comprises the following elements connected in sequence: a sample injection tube, a nucleic acid extraction-free reagent chamber, a first buffer solution chamber, a nucleic acid amplification reagent chamber, a second buffer solution chamber, and a uniform mixing chamber, all of which are flexible reagent chambers, as well as a sample loading recess and a chromatography test strip; a head end of the sample loading recess is connected to the uniform mixing chamber, and a tail end of the sample loading recess is connected to the chromatography test strip; and the squeezing block can move from one end of the upper cover towards the other end, and is used for squeezing a liquid in the testing main body to force the liquid to flow downstream. Because the liquid is squeezed to flow downstream only when the squeezing block is manually pushed to move, controllability of the testing process can be achieved.

The invention discloses a navigation system, a storage medium and a method for portal vein and branch vein puncture, and belongs to the technical field of medical instruments. The system comprises a modeling module used for constructing a three-dimensional blood vessel model containing a target belonging branch; the image fusion and navigation display module is used for performing registration fusion on the model and the intraoperative real-time image to generate an augmented reality navigation interface; the surgical instrument tracking and positioning module is used for acquiring and displaying the spatial pose of the instrument in real time; and the intelligent distinguishing module is used for analyzing the physical sensing signal of the instrument and automatically generating a distinguishing signal of entering a blood vessel cavity. The technical problems that an existing navigation system cannot clearly recognize deep branch blood vessels, is not intuitive in guidance and lacks objective successful criteria are solved, accurate, intuitive and intelligent surgical navigation of portal vein specific branches is achieved, and the system is particularly suitable for clinical scenes needing high-quality portal vein blood samples, such as free nucleic acid detection.

The present application relates to the technical field of nucleic acid testing. Disclosed is a multi-target microfluidic chip having a novel liquid distribution method, the multi-target microfluidic chip comprising a reaction layer, a sealing layer and a liquid distribution layer, wherein the reaction layer is provided with a plurality of amplification reaction chambers, a plurality of sample loading buffer reservoirs and a plurality of test strip storage slots; a vertically arranged sample inlet, a plurality of sample distribution channels and a plurality of sample vertical flow channels are provided within the liquid distribution layer, the sample inlet and the sample vertical flow channels vertically running through the top surface and the bottom surface of the liquid distribution layer; and the sample vertical flow channels, the sample distribution channels and the amplification reaction chambers are correspondingly positioned, the plurality of sample distribution channels are all in communication with the sample inlet, the plurality of sample distribution channels are of the same size and are arranged at intervals along a conical surface, and the plurality of sample vertical flow channels are also of the same size and are in communication with the amplification reaction chambers. Such a configuration allows a sample to be completely and equally distributed, enables multiple parallel reactions to happen simultaneously, and maximizes the amount of the sample entering the amplification reaction chambers to reduce liquid loss, thereby improving the testing accuracy.

This invention discloses a household-use, fully enclosed thermos for COVID-19 nucleic acid testing. Based on loop-mediated isothermal amplification (RT-LAMP) technology, it integrates the entire nucleic acid testing process and post-testing sterilization into a constant-temperature, negative-pressure thermos. The testing process is observed and analyzed via a mobile app using a smartphone camera, and the results are displayed on a screen. The device can also connect to a mobile app to upload test results. The testing process requires the necessary experimental materials for RT-LAMP. This invention significantly reduces costs compared to the RT-PCR method, while offering portability and a simpler operating procedure.

The invention discloses a nucleic acid swab detection consumable, and belongs to the field of nucleic acid detection. Comprising a swab tube, a PCR tube, a piston push rod, a pressing push rod, a swab tube cover plug, a PCR tube plug and a freeze-drying reagent ball, the swab tube is used for containing a sample preservation solution and containing a swab sample, the freeze-drying reagent ball is arranged in the PCR tube, the PCR tube plug covers a tube opening of the PCR tube in a sealed mode, and the swab tube cover plug covers a tube opening of the swab tube in a sealed mode; the swab tube cover, the pressing push rod and the swab tube cover plug form an integrated assembly, the pressing push rod and the swab tube cover plug can be driven to be synchronously separated from the swab tube by unscrewing the swab tube cover, and the pressing push rod and the piston push rod are of a split structure. According to the kit, the detection process is integrated, the swab tube, the PCR tube and the pushing structure are integrated, and compared with a traditional split detection consumable, the kit has the advantages that the occupied space is greatly reduced, carrying and on-site rapid detection are facilitated, and the kit does not need to depend on a professional laboratory environment.

The present application relates to a chip, a preparation method therefor, and an application thereof. The chip of embodiments of the present application comprises: a substrate having a surface grafted with an amino group, the amino group being a primary amino group or a secondary amino group; and a first compound linked to the surface via the amino group, the first compound comprising the following structure, i.e., formula (1) or formula (2), wherein R1 and R2 are each independently selected from —OH or C1-C5 alkoxy, and n is 1 or 2. The chip can be used for a nucleic acid test, such as single-molecule sequencing and next-generation sequencing.

The present application relates to the technical field of nucleic acid testing, and discloses an integrated nucleic acid testing apparatus compatible with multiple sample types, and a nucleic acid testing method. The nucleic acid testing apparatus comprises a plurality of chambers, wherein the top end of a nucleic acid extraction chamber is provided with a saliva sample compartment, a plasma sample compartment, and a nasopharyngeal swab sample compartment. A test strip testing chamber comprises a first test strip chamber and a second test strip chamber, and the second test strip chamber is slidably connected to the first test strip chamber. Each chamber is internally provided with a through hole penetrating the two end surfaces thereof. The side wall of each through hole is provided with puncture structures extending towards the top surface thereof. The bottom end of each through hole is sealed by a sealing film; and the puncture structures are used for puncturing the corresponding sealing film. The test paper is fixed in a test paper storage slot of the second test paper chamber. The test paper storage slot is in communication with the through hole of the first test paper chamber. The test strip testing chamber is provided with a test result output window for outputting a test result. The testing is easy to operate, and the testing process is in a sealed state, thereby avoiding environmental pollution and allowing compatibility of different types of samples.

1. Name of the product in this design: Receptacle for a test kit. 2. Purpose of this design: as a container for receiving reagents required for nucleic acid testing and for receiving samples to be tested in a test kit. 3. The key design feature of this product is its shape. 4. The image or photograph that best illustrates the design's key points: a 3D model.

The present application relates to the technical field of nucleic acid testing, and discloses a rotary structure-based universal automated nucleic acid extraction module, comprising a liquid storage layer and a flow channel layer which are vertically arranged. The nucleic acid extraction module is internally provided with a lysis buffer chamber, a binding buffer chamber, a magnetic bead chamber, a front wash buffer chamber, a rear wash buffer chamber, an elution buffer chamber, a sample processing chamber, a waste liquid chamber, and a sample loading chamber. A valve core is mounted in the sample processing chamber; a sample processing chamber piston is mounted in a core cavity of the valve core; and a side wall of the core cavity is provided with a liquid transfer port in communication with the core cavity. The top surface of the flow channel layer is correspondingly provided with flow channels in communication with the lysis buffer chamber, the binding buffer chamber, the magnetic bead chamber, the front wash buffer chamber, the rear wash buffer chamber, the elution buffer chamber, the waste liquid chamber, and the sample loading chamber, respectively; and the valve core rotates around its own axis and can be in communication with different flow channels. The switching of the liquid flow channels is convenient, the structure is simpler, the assembly is easier, the cost is lower, the volume is smaller, and the weight is lighter.

Assembly of use in high-throughput nucleic acid testing system including a tub and a deck. The tub includes a floor and four walls extending upwardly from the floor to define an interior. The four walls include a first wall having a first rim at a top portion thereof, a second wall having a second rim at a top portion thereof, a third wall having a third rim at a top portion thereof, and a fourth wall having a fourth rim at the top portion thereof. The first, second, third, and fourth rim define an opening to access the interior of the tub. The deck is shaped to be received in the opening of the tub. The deck includes a first snap-fit arm extending from a first edge and configured to extend through the first receiving hole to couple the deck and the tub.

A nucleic acid test cartridge and a nucleic acid test method. The cartridge is provided with a sample inlet and a sample chamber in communication with the sample inlet. A lysis chamber, an elution chamber, and a reaction chamber, which are sequentially in communication by means of a flow channel, are arranged inside the cartridge. The sample chamber is in communication with the lysis chamber by means of a flow channel. A magnetic bead chamber is further arranged inside the cartridge. The magnetic bead chamber is in communication with the lysis chamber by means of a flow channel. A waste liquid chamber is further arranged inside the cartridge. The waste liquid chamber is in communication with the lysis chamber and the elution chamber by means of flow channels. The cartridge is provided with a first air vent in communication with at least one of the sample chamber and the lysis chamber, a second air vent in communication with the elution chamber, and a reagent inlet in communication with the lysis chamber and the elution chamber.

The invention relates to a chimeric annular sgRNA-CRISPR-Cas12a system and application of the chimeric annular sgRNA-CRISPR-Cas12a system, and belongs to the technical field of biological detection. The system comprises a Cas12a protein, a DNA-RNA (deoxyribonucleic acid-ribonucleic acid) chimeric annular sgRNA, a target sgRNA, an annular DNA substrate and a reporter molecule, an autocatalytic cascade reaction is triggered through a topological gating mechanism of the annular sgRNA, and the trans-cleavage activity of Cas12a is activated. The system can be directly used for pre-amplification-free nucleic acid detection (such as detection of virus, tumor genes and pathogen sequences) and conditional gene editing. Amplification-free detection is realized, the sensitivity reaches a femtomole level, and the detection time is shortened to 30-60 minutes; gene editing can be accurately activated based on an endogenous nucleic acid marker, so that controllable editing is realized; the detection and editing functions are integrated, the specificity is high, the stability in a complex matrix is good, and the kit is suitable for bedside diagnosis and precise treatment scenes.