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Method for amplifying target nucleic acid sequence by using cross primer and kit for amplifying target nucleic acid sequence and application thereof

A cross-primer amplification, target nucleic acid sequence technology, applied in biochemical equipment and methods, microorganism determination/inspection, etc., can solve problems such as high specificity and complex cycle process

Active Publication Date: 2010-02-03
USTAR BIOTECHNOLOGIES (HANGZHOU) CO LTD
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Problems solved by technology

The transcription-dependent amplification system has high amplification efficiency and high specificity, but its cycle process is complicated, requiring repeated addition of reverse transcriptase and T7 RNA polymerase, so further research is needed

Method used

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  • Method for amplifying target nucleic acid sequence by using cross primer and kit for amplifying target nucleic acid sequence and application thereof
  • Method for amplifying target nucleic acid sequence by using cross primer and kit for amplifying target nucleic acid sequence and application thereof
  • Method for amplifying target nucleic acid sequence by using cross primer and kit for amplifying target nucleic acid sequence and application thereof

Examples

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Embodiment 1

[0124] The detection of embodiment 1 chlamydia trachomatis

[0125] Since the 1990s, Chlamydia trachomatis has surpassed Neisseria gonorrhoeae as the most common pathogen of genitourinary tract infections. With the increasing incidence of Chlamydia trachomatis infection and its complications, it has become an increasing threat to human reproductive health. In 1995, the World Health Organization estimated that there were 90 million Chlamydia trachomatis patients worldwide, second only to HIV infection. Therefore, it is of great significance to realize the rapid diagnosis of Chlamydia trachomatis. By applying the patent, the DNA of the chlamydia trachomatis virus can be quickly detected. The specific design of related primers is as follows: we select the gene fragment to be amplified on the DNA sequence of Chlamydia trachomatis, and design specific primers according to the sequence. The reaction system is as follows:

[0126] Stripping primers F and R 0.05 micromole per lite...

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Abstract

The invention relates to a novel technique for amplifying a nucleic acid sequence, in particular to a method for amplifying a target nucleic acid sequence at a constant temperature by using a cross primer. The invention also relates to a method for marking the amplified target nucleic acid sequence in an amplified reaction and a method for quickly detecting the target nucleic acid sequence. The invention also relates to a kit used in quick nucleic acid diagnosis, the application of the kit in the aspect of detecting the nucleic acid of pathogenic microorganisms including bacteria, viruses andthe like, and the application of the kit in gene diagnoses correlative with human genetic diseases.

Description

technical field [0001] The present invention relates to a new amplification technique for nucleic acid sequences, more specifically, to a method for using cross primers to amplify target nucleic acid sequences at constant temperature. The invention also relates to a method for marking and amplifying the target nucleic acid sequence in the amplification reaction and a rapid detection method for the target nucleic acid sequence. [0002] The present invention also relates to a kit for rapid nucleic acid diagnosis and its application in nucleic acid detection of pathogenic microorganisms such as bacteria and viruses, as well as its application in gene diagnosis related to human hereditary diseases. Background technique [0003] In the past 30 years, the global epidemic trend shows that difficult and unculturable pathogenic microorganisms have become the main source of infectious and infectious diseases. Some conventional detection methods for pathogenic bacteria (for example, ...

Claims

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Application Information

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IPC IPC(8): C12Q1/68C12Q1/70C12Q1/04
Inventor 尤其敏胡林徐高连石翊轩
Owner USTAR BIOTECHNOLOGIES (HANGZHOU) CO LTD
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