Digital isothermal nucleic acid detecting device and detecting method thereof

A detection device and nucleic acid technology, which is applied in the field of biomedical nucleic acid detection devices, can solve the problems of long detection time, high requirements for instruments, and inability to meet the needs of rapid detection of nucleic acids on site, and achieve low equipment requirements, simple chip structure, and portability and the effect of miniaturization
CN105505761AInactive Publication Date: 2016-04-20SUZHOU INST OF BIOMEDICAL ENG & TECH CHINESE ACADEMY OF SCI
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
SUZHOU INST OF BIOMEDICAL ENG & TECH CHINESE ACADEMY OF SCI
Publication Date
2016-04-20
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention provides a digital isothermal nucleic acid detecting device which comprises a micro-fluidic chip, a temperature control system and a pressure driving system. A microfluidic channel for microsphere forming and a reaction section for nucleic acid amplification are formed by the micro-fluidic chip through sequential laminating of a substrate layer, a channel layer arranged on the substrate layer and a cover plate layer arranged on the channel layer. The temperature control system comprises a lower press plate for applying pressure to the substrate from bottom to top and an upper press plate for applying pressure to the cover plate layer from top to bottom, and temperature sensing chips and temperature control heating elements for heating the reaction section are arranged in the upper press plate and the lower press plate. The pressure driving system is connected to the micro-fluidic chip and used for applying pressure to the channel layer in the micro-fluidic chip so that liquid to be detected can flow into the reaction section from the inflow end. According to the scheme, microsphere preparation and nucleic acid amplification and detection are integrated through the digital isothermal nucleic acid detecting device and the detection method, and the advantages of being high in sensitivity and the like are achieved.
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Description

technical field

[0001] The invention relates to a biomedical nucleic acid detection device, in particular to a microfluidic device for nucleic acid digital isothermal amplification and a detection method thereof. Background technique

[0002] Current biomedical research is going deep into the molecular level from the whole and cellular level. Nucleic acid is an important class of biomolecules in cells, which participates in the regulation of most functions of cells, such as gene expression and silencing, organelle composition, and cell behavior regulation. Understanding the content and distribution of nucleic acids in different cells is crucial to the in-depth study of their functions and the biological significance behind them, and is of great significance to the diagnosis and treatment of malignant tumors, AIDS, and genetic diseases.

[0003] Existing nucleic acid detection mainly adopts polymerase chain reaction (PCR), which has high sensitivity and good specificity, and...

Claims

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