83 results about "Lactoperoxidase" patented technology

PCT No. PCT / SE97 / 00098 Sec. 371 Date Jul. 22, 1998 Sec. 102(e) Date Jul. 22, 1998 PCT Filed Jan. 22, 1997 PCT Pub. No. WO97 / 26908 PCT Pub. Date Jul. 31, 1997Use of an antibacterial system comprising lactoperoxidase and a peroxide donor for preparing a preparation for prophylactic or therapeutic treatment "in vivo" of an infection caused by the bacteria Helicobacter pylori existing in the stomach, which preparation is completed by the presence of thiocyanate in an antibacterial level, and eventually in the presence of lactoferrin. A daily dose for human treatment is 1.2-1.6 grams of the system taken 3 times a day.

The present invention involves the discovery that various liquid (whey) streams drained or expelled from cheese curd after salt addition in the preparation of cheese contain enriched levels of bioactive proteins such as lactoferrin, lactoperoxidase, immunoglobulins, and growth factors. According to the invention, these proteins may be further enriched through manipulation of the cheese salting process as described herein. The methods of the invention may be used to produce various whey products with enriched levels of all the above bioactive proteins present and, through manipulation of salting conditions, to enrich these proteins selectively.

The invention provides a purification method for recombination human lactoferrin, animal lactoferrin and lactoperoxidase. The method comprises: taking a milk sample containing recombination human lactoferrin as a raw material, pre-treating to obtain degreased milk, performing cation exchange chromatographic separation on the obtained degreased milk, then under different salt concentration conditions, performing elution separation on lactoferrin and lactoperoxidase, so as to obtain high-purity lactoferrin and lactoperoxidase, and further performing separation on the solution rich in lactoferrin by utilizing hydrophobic interaction chromatography to respectively obtain recombination human lactoferrin and animal source lactoferrin both with the purity of 99% or more. The purity of recombination human lactoferrin obtained by utilizing the purification method provided by the invention is 99% or more, and also recombination human lactoferrin has the physical and chemical activities and physiological functions all similar to those of natural human lactoferrin; and the technology is a one-step purification process, so that the purification method is low in cost, simple and suitable for industrialized large-scale production.

The invention relates to an oral spray for inflammatory diseases of the oral cavity and a preparation method thereof. The oral spray comprises the following components: 0.1-5% of water-soluble chitosan, 0.5-5% of propolis extract, 0.1-1% of lactoferrin, 0.01-0.5% of lactoperoxidase, 0.1-0.5% of lysozyme, 0.01-0.1% of immunoglobulin, 1-10% of bovine colostrum extract, 1-10% of anti-caries sweetener, 0.8-1.2% of cold aromatic, 0.1-1% of preservative and the balance of water for injections. The preparation method is characterized by mixing the components, stirring uniformly, filling into an oral sprayer, and sterilizing at 85 DEG C for 15 minutes to obtain the finished product. The oral spray has the effect of immediately and rapidly treating inflammatory diseases of the oral cavity and the functions of resisting bacteria and viruses, relieving pains, reducing gum inflammation and blooding, promoting the healing of oral ulcer wounds, treating acute and chronic pharyngitis, moistening and freshening the oral cavity and removing halitosis. The oral spray has the advantages of simple and convenient large-scale production process and no environmental pollution in the production process.

The invention provides a method for producing lactoferrin and lactoperoxidase. The method comprises the following steps: an emulsion used as a raw material undergoes ion exchange treatment by using a macro-porous cation exchange resin column to make lactoferrin and lactoperoxidase in the emulsion be adsorbed to the resin column, wherein the temperature of the emulsion is not greater than 55DEG C, and the stay time of the emulsion in the resin column is controlled to be 1-6min; different concentrations of aqueous solutions of sodium chloride or potassium chloride are adopted to carry out gradient elution on the resin column, and a lactoperoxidase-containing first eluate and a lactoperoxidase-containing second eluate are collected; and the first eluate and the second eluate respectively undergo membrane filtration concentration to form first eluate concentrate and second eluate concentrate with the volumes being 1 / (8-12) of original volumes, and the first eluate concentrate and the second eluate concentrate respectively undergo filter washing desalination in order to obtain lactoperoxidase concentrate and lactoferrin concentrate.

The invention discloses oral care composition for treating xerostomia and an application of the oral care composition in toothpaste. The oral care composition for treating xerostomia contains Chinese herbal medicine extract and bio-enzyme, wherein the Chinese herbal medicine extract is one or more of dendrobium extract, radix polygonati officinalis extract and radix ophiopogonis extract; the bio-enzyme is one or more of lysozyme, lactoperoxidase, amylase, amyloglucosidase and glucose oxidase. The oral care composition has the effect of relieving xerostomia, on one hand, the oral care composition can stimulate salivary secretion, and on the other hand, the oral care composition contains a component similar to protein or enzyme in saliva, thereby being capable of playing the role of artificial saliva, having the sterilization effect and being capable of relieving discomfort caused by xerostomia after use.

The invention discloses a plant bactericide containing a milk protein composition, belonging to the field of plant disease control. The plant bactericide consists of the following main components in percentage by mass: 0.01-6% of a milk protein composition, 1-9% of iodide, 0.1-8% of rhodanate, 2-12% of a hydrogen peroxide source material, 6-25% of an auxiliary agent and the balance of water. According to the plant bactericide, a mixture of lactoperoxidase and lactalbumin, a mixture of hydrogen peroxide and sodium percarbonate as well as a special auxiliary agent are used as important bacteriocidal substances, so that the bactericidal performance and bactericidal effect of the plant bactericide are significantly improved, and the plant bactericide has the characteristics of strong bactericidal activity, good stability, long bactericidal lasting period and strong environmental adaptability, and can meet actual requirements in the field of plant disease control.

Provided are methods for aiding in diagnosing autoimmune diseases in a mammal, comprising contacting a sample of tear from the mammal with an antibody that specifically binds to a first polypeptide selected from the group Ctss, Ctsh, Ctsr, Ctsw, Ctsz, Ifng, Il16ra, Il10, Il10ra, Il15, Tnfa, Apo-F, or Lcn-2 or a second polypeptide selected from the group lactoperoxidase, lactoferrin or lysozyme under conditions favoring the formation of an antibody-polypeptide complex, and determining the amount of complex formed, wherein an increased formation of antibody-first-polypeptide complex or a decreased formation of antibody-second-polypeptide complex as compared to a suitable control, indicates a likely positive diagnosis of an autoimmune disease for the mammal, thereby aiding in the diagnosis. Methods of treating the autoimmune diseases are also provided.

It is intended to provide an osteogenesis promoter capable of promoting osteogenesis by promoting the differentiation of osteoblasts, and foods, drinks, drugs or feeds for promoting osteogenesis. Namely, an osteogenesis promoter capable of promoting osteogenesis by promoting the differentiation of osteoblasts which comprises, as the active ingredient, lactoperoxidase and / or a digestion product obtained by digesting lactoperoxidase with a protease such as trypsin; and foods, drinks, drugs or feeds for promoting osteogenesis containing lactoperoxidase and / or its digestion product.

The invention discloses a buffalo milk active-bacteria whey beverage and a preparation method thereof. The whey beverage is obtained through the steps of: mixing byproduct whey generated in the preparation process of buffalo milk cheese with fermented buffalo sour milk in a certain ratio, appropriately adding sugar, a stabilizer and spice to obtain a mixture, then concocting, homogenizing and filling the mixture, so as to obtain the buffalo milk active-bacteria whey beverage. The whey beverage has the complete nutritional ingredients of the whey and the sour milk, contains protein, various amino acids, vitamins and mineral substances needed by human bodies, and especially contains various active proteins such as alpha-lactalbumin, beta-lactoglobulin, immune globulin, lactoferrin, lactoperoxidase and active lactobacillus which are contained in the whey; and the beverage is unique in flavor, exquisite in mouth feel, rich in nutrition, and capable of improving the gastrointestinal function and enhancing the immunity.

The invention relates to a medical gargle product for xerostomia and a preparation method thereof. The gargle comprises the following components: 0.1-5% of water-soluble chitosan, 0.1-1% of lactoferrin, 0.01-0.5% of lactoperoxidase, 0.1-0.5% of lysozyme, 0.01-0.1% of immunoglobulin, 1-10% of bovine colostrum extract, 1-10% of anti-caries sweetener, 0.1-1% of preservative and the balance of water for injections. The preparation method is characterized by firstly preparing a water-soluble chitosan solution, a solution of lactoferrin, immunoglobulin, anti-caries sweetener, preservative and bovine colostrum extract and a solution of lysozyme and lactoperoxidase respectively with part of the water for injections, mixing the prepared three solutions with the rest of the water for injections, stirring uniformly, subpackaging, and then sterilizing at 85 DEG C for 15 minutes to obtain the finished product. The gargle has the effects of immediately and rapidly moistening, comforting and freshening the oral cavity and the functions of resisting bacteria and viruses, preventing dental plaque, reducing gum inflammation and blooding and oral ulcer, promoting the healing of wounds in the oral cavity and preventing oral candidiasis. The gargle has the advantages of simple and convenient large-scale production process and no environmental pollution in the production process.

The invention discloses a plant bactericide composition based on a lactoperoxidase system, and belongs to the field of plant disease control. The plant bactericide composition is prepared from the following components by mass percentage: 36-50% of biological bactericide substrate composition, 36-50% of oxidizing agent composition and 8-25% of sterilization additive composition. According to the plant bactericide composition, a lactoperoxidase protective agent is added in the biological bactericide substrate composition, a sodium carbonate stabilizer is added in the oxidizing agent composition, and a proper amount of sterilization additive is added as the important substance of the plant bactericide composition, so that synergistic interaction of various effective ingredients in the composition is realized, and the control efficiency on plant pathogenic microorganisms is remarkably improved. The plant bactericide composition has the characteristics of being strong in bactericidal power, good in sterilizing effect, long in lasting period and free from environmental influence, and meets the actual demand in the field of plant disease control.

The invention provides milk and a preparing method thereof. The milk contains 2000-3500 mg / L of beta-lactoglobulin, 500-800 mg / L of alpha-lactoglobulin, 20 mg / L or above of lactoferrin, 30 U / L or above of lactoperoxidase, lower than 12 mg of furosine in every 100 g of protein, 1000 micrograms per liter or above of immune globulin lgA, 81000 micrograms per liter or above of immune globulin lgG, 1000 micrograms per liter or above of immune globulin lgM, lower than 1 cfu / mL of total bacterial colonies, lower than 1 cfu / mL of total spores and lower than 1 cfu / mL of total heat resistance spores. Nutrient components in original milk are greatly maintained in the milk, the quantity of residual microorganisms is low, the food safety is high, the shelf-life is long, and the flavor and the mouthfeelare excellent.

The invention discloses a method for extracting lactoperoxidase by the ultrafiltration assisted two aqueous phase extraction technology. The method comprises the steps of using bovine whey as the raw material; enriching all lactoperoxidase from bovine whey into a salt phase by the two aqueous phase extraction technology; processing the salt phase solution through an ultrafiltration membrane to remove small impure protein; further purifying lactoperoxidase; desalting through a dialysis bag; then freezing and drying, so as to prepare a high-activity lactoperoxidase product which can be used in natural food additives. With the adoption of the method, lactoperoxidase with natural antibacterial activity can be directly prepared and directly added as a preservative into foods or toothpaste and other daily supplies.

Provided is a prophylactic agent for an autoimmune disease, including a fraction of a milk-derived basic protein, lactoperoxidase, and lactoferrin as active ingredients. The agent can be taken on a daily basis, and even if it is taken over a long period of time, its safety is high, and hence, an autoimmune disease such as type I diabetes mellitus or rheumatoid arthritis, which could not be effectively prevented or treated by a conventional method, can be prevented.

The invention relates to a medical gel product for xerostomia and a preparation method thereof. The medical gel product for xerostomia comprises the following components: 0.1-5% of water-soluble chitosan, 0.3-1.5% of water-soluble polymer gel material, 1-10% of gel viscosity regulator, 0.1-1% of lactoferrin, 0.01-0.5% of lactoperoxidase, 0.1-0.5% of lysozyme, 0.01-0.1% of immunoglobulin, 1-10% of bovine colostrum extract, 1-10% of anti-caries sweetener, 0.1-1% of neutralizer and the balance of purified water. The preparation method is characterized by firstly mixing the components, subpackaging and then sterilizing with ethylene oxide to obtain the finished product. The medical gel product for xerostomia has the functions of keeping long-acting moisture in the oral cavity, comforting and freshening the oral cavity, resisting bacteria and viruses, preventing dental plaque, reducing gum inflammation and blooding and oral ulcer, promoting the natural healing process of the oral cavity and preventing oral candidiasis. The medical gel product for xerostomia has the advantages of simple and convenient large-scale production process and no environmental pollution in the production process.

Peroxidase systems such as the Lactoperoxidase system (LPS) are combined with antimicrobial fatty acids or fatty acid derivatives to produce a synergistic antimicrobial composition. These compositions are disclosed as being useful for rendering surfaces, and also products such as food or cosmetics, resistant to microbes. A preferred form of the composition contains lactoperoxidase, monolaurin or sodium dodecyl sulphate, a source of peroxide, and thiocyanate ions.

The present invention relates to the use of hydrogen peroxide producing enzyme for manufacturing of a medicament for treatment and / or prevention of otitis media (inflammation in the middle ear), preferably in children. This enzyme is preferably NADH oxidase. The medicament is intended to be administered with lactoperoxidase into the nasal cavity.

The invention relates to a protein material includes angiogenin and / or angiogenin hydrolysate in an amount of 2 to 15 mg / 100 mg, and lactoperoxidase and / or lactoperoxidase hydrolysate, in the mass ratio to angiogenin and / or angiogenin hydrolysate of 0.3 to 20.

The invention relates to a powdered milk product includes angiogenin and / or angiogenin hydrolysate in an amount of 1.4 to 24 mg / 15 g, and lactoperoxidase and / or lactoperoxidase hydrolysate in the mass ratio to the angiogenin and / or angiogenin hydrolysate of 0.35 to 10.

The invention relates to tartaric acid gelatin and a preparation method thereof. Each part of gelatin comprises an ingredient A (deionized water and 2.0 percent to 2.2 percent of methoxy ethylene / kuei-diene-maleic acid gel-linked polymers), an ingredient B (1.0 percent and 1.1 percent of sodium hydroxymethyl glycinate), an ingredient C (a proper amount of sodium hydroxide used for regulating the pH value) and an ingredient D (10 percent to 14 percent of a mixture of black fruit cranberry extracts, sugar cane extracts, orange extracts, lemon extracts and hard maple extracts, 5 percent to 7 percent of cytosol extracts, 3 percent to 5 percent of water yucca extracts, 5 percent to 6 percent of northern wild corn extracts, 0.5 percent to 0.6 percent of tocopherol peptide and 0.9 percent to 1.0 percent of a mixture of glucose, glucose oxidase and lactoperoxidase). The preparation method comprises the following steps: carrying out dispersing cooling on the ingredient A; heating the ingredient A for an hour to 80 DEG C for dissolving the ingredient A; cooling the ingredient A to 35 DEG C; adding the ingredient B; using the ingredient C for regulating the acidity to 4.5 to 5.0; and adding the ingredient D. When the prepared tartaric acid gelatin is applied onto the skin, the stimulation of the relative pure solution of the tartaric acid can be reduced, and the tartaric acid gelatin can perfectly reduce fine wrinkles, can generate the oxidation resistance effect, and can slow down the skin aging.

The invention relates to provide a fermented milk product includes angiogenin and / or angiogenin hydrolysate in an amount of 0.9 mg / 100 g to 150 mg / 100 g, and lactoperoxidase and / or lactoperoxidase hydrolysate in the mass ratio to the angiogenin and / or angiogenin hydrolysate of 0.3 to 23.

The invention relates to a milk product and a preparation method thereof, a dairy product and a preparation method thereof, in particular to the milk product with high nutritional activity and high biological safety and the preparation method thereof. The preparation method of the milk product comprises the following steps: (1) purifying milk; (2) carrying out RO membrane concentration; (3) degassing; (4) separating; (5) sterilizing the single cream at high temperature; (6) physically degerming the skimmed milk; (7) backfilling; (8) homogenizing; and step (9) carrying out sterilization treatment. The milk product contains the total number of bacterial colonies which are less than or equal to 5000 cfu / mL in the 42nd day stored under the condition of 0-10 DEG C, furosine which is less than or equal to 11 mg / 100 g protein, lactoperoxidase of 1500-20000 U / L, lactoferrin of 35-120 mg / L, immunoglobulin of 100-1000 mg / L and lysozyme of 200-800 [mu] g / L.

The invention relates to a cheese includes angiogenin and / or angiogenin hydrolysate in an amount of 6.5 mg / 100 g to 160 mg / 100 g, and lactoperoxidase and / or lactoperoxidase hydrolysate in the mass ratio to the angiogenin and / or angiogenin hydrolysate of 0.3 to 33.

The invention relates to a drink includes angiogenin and / or angiogenin hydrolysate in an amount of more than 0.8 mg / 100 ml and not more than 150 mg / 100 ml, and lactoperoxidase and / or lactoperoxidase hydrolysate in the mass ratio to the angiogenin and / or angiogenin hydrolysate of 0.3 to 23.

The invention provides a method for detecting lactoperoxidase (LPO) in milk products with the advantages of high sensitivity, low error, simplicity and rapidness. The method comprises the following steps of: (1) taking two cuvettes used as a contrast tube and a test tube respectively, and adding 2.2mL phosphate buffer solution (pH 5-6) respectively; (2) adding 0.5-4mM ABTS solution 0.7mL respectively; (3) adding 1-30mM H2O2 solution 0.1mL respectively; (4) adding 0.2mM dipotassium hydrogen phosphate solution 0.05mL to the contrast tube, and adding 0.05mL sample to the test tube; and (5) mixing, zeroing a blank tube, continuously detecting the absorbance at 416nm in the first 120s at room temperature with spectrophotometer using dynamic curve mode, outputting and printing to obtain one curve approximate to straight line can be obtained for each detection, calculating the slope y of the curve, and calculating enzyme activity x according to the equation x=256.4y+0.0256. The invention uses ABTS reagent, and has the advantages of low cost and easiness for generalization.

The present invention involves the discovery that various liquid (whey) streams drained or expelled from cheese curd after salt addition in the preparation of cheese contain enriched levels of bioactive proteins such as lactoferrin, lactoperoxidase, immunoglobulins, and growth factors. According to the invention, these proteins may be further enriched through manipulation of the cheese salting process as described herein. The methods of the invention may be used to produce various whey products with enriched levels of all the above bioactive proteins present and, through manipulation of salting conditions, to enrich these proteins selectively.