Trichina antibody double-hole rapid detection card and detection method

Abstract

The invention discloses a trichina antibody double-hole rapid detection card and a detection method, the trichina antibody double-hole rapid detection card comprises a PVC base plate, a water absorption pad, a nitrocellulose membrane, a marker pad, a sample pad, a filter substance pad and a dilution pad which are installed in a shell, the nitrocellulose membrane is fixed on the PVC base plate, the left end of the nitrocellulose membrane is in lap joint with the water absorption pad, the right end of the nitrocellulose membrane is in lap joint with the dilution pad, a marker pad is arranged at the right position of the middle of the nitrocellulose membrane, a sample pad is arranged on the marker pad, a detection line and a quality control line are arranged between the water absorption pad and the marker pad on the nitrocellulose membrane, and a window hole, a sample adding hole, a cleaning hole and a quantitative marking line are formed in the shell. A sample is added through the middle sample adding hole, and a diluent is added through the rear cleaning hole, so that the sensitivity and the precision of a detected object can be improved, and the characteristics of rapidness, simplicity and convenience of a rapid diagnosis detection method are kept.

Description

technical field [0001] The invention relates to a detection card, in particular to a double-hole rapid detection card for trichinella spiralis antibody and a detection method. Background technique [0002] Trichinellosis (Trichinellosis) is caused by Trichinella spiralis, a zoonotic parasitic disease that seriously endangers human health. Trichinella spiralis can go through different stages in the host body: adult, newborn larva and muscle larva, respectively Parasitizes in the small intestine, blood vessels and striated muscles of the host. The main source of human trichinellosis in pork and its products with cysts of Trichinella muscle larvae. The neonatal larva is the only developmental stage in the entire life cycle of Trichinella spiralis that moves in the blood without parasitizing in the host cell, and is the main target stage for host immunity. During the transition of each life stage of Trichinella spiralis, the secretion product (ES) produced and excreted on its ...

AI Technical Summary

Problems solved by technology

[0004] At present, the detection of Trichinella antibody enzyme-labeled kits on the market requires equipment such as enzyme-labeled readers, plate washers, and incubation reactions. The operation is cumbersome and takes a long time, and it can only be operated by professional technicians in the laboratory.

In addition, the detection of samples also requires complex pre-processing, such as blood collection, serum separation, etc.; and although rapid colloidal gold detection is convenient and does not require professional technicians and working environments, its detection sensitivity is low, and it can only be determined qualitatively and visually.

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