Fluorescence immunochromatography detection test paper strip of trichinellosis as well as preparation method and application thereof

A technique for fluorescence immunochromatography and detection test paper, which is applied in fluorescence/phosphorescence, material analysis by optical means, measurement devices, etc. Effect

Active Publication Date: 2019-09-10
JILIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, due to the limitation of sensitivity and accuracy of colloidal gold detection, it can only be used in some detection items with low requirements.

Method used

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  • Fluorescence immunochromatography detection test paper strip of trichinellosis as well as preparation method and application thereof
  • Fluorescence immunochromatography detection test paper strip of trichinellosis as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Embodiment 1. Fluorescent immunochromatographic detection test strip for trichinosis.

[0031] A fluorescent immunochromatographic detection test strip for trichinellosis described in this embodiment includes a sample pad, a binding pad, a chromatographic membrane, a water-absorbing pad and a bottom plate, and the binding pad is marked with time-resolved fluorescent microspheres coupled with goat antibody. Pig IgG; the sample pad is provided with a sample hole; the chromatographic membrane is provided with a detection line and a quality control line, wherein the detection line is sprayed with Trichinella spiralis muscle larva ES mixed antigen, and the Trichinella spiralis muscle larvae ES mixed antigen The antigen is composed of the ML-ESP antigen of the excreted secretion of the muscle larvae of Trichinella spiralis and the iML-ESP antigen of the excreted secretion of the muscle larvae of the intestinal stage of Trichinella spiralis; the quality control line is sprayed ...

Embodiment 2

[0032] Example 2. The preparation method of the fluorescent immunochromatographic detection test strip for trichinellosis.

[0033] 1) Preparation of ES mixed antigen from Trichinella spiralis muscle larvae: ML-ESP antigen excreted by Trichinella spiralis muscle larvae and iML-ESP antigen excreted by Trichinella spiralis intestinal stage muscle larvae were mixed according to the mass ratio of 1:1 to obtain Trichinella spiralis Caterpillar muscle larva ES antigen; the specific method is:

[0034] Trichinella spiralis muscle larvae excretion secretion (ML-ESP) antigen preparation:

[0035] The collected Trichinella spiralis muscle larvae were washed three times with a culture solution containing penicillin (500U / mL) and streptomycin (500U / mL) (natural precipitation, each time for 20min), and then placed in a culture medium containing penicillin (250U / mL) and streptomycin (250U / mL) in culture medium (density 5000 / mL), 37°C, 10% carbon dioxide, cultured for 18h-24h and then colle...

Embodiment 3

[0073] Example 3. The application of trichinellosis fluorescent immunochromatographic detection test strip in the detection of trichinellosis.

[0074] Sample dilution: Dilute Trichinella antibody-positive serum (such as pig serum infected with Trichinella), negative serum (such as normal pig serum) and serum to be tested (such as pig serum) samples with sample diluent, respectively, to 95 μL diluent Add 5 μL of porcine serum and mix with a pipette. Place the test strip flat on the operating table with the sample well facing up, add 100 μL of diluted serum sample into the well, and let it stand at room temperature for 5-10 minutes. The sample diluent is prepared by adding 9 g of sodium chloride (NaCl), 2 g of bovine serum albumin (BSA), and adding double distilled water to 1000 mL. Use UV lamp 360-370nm to irradiate the test strip to observe the results.

[0075] The results should be judged within 20 minutes after adding the sample. If the quality control line and detectio...

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Abstract

The invention provides a fluorescence immunochromatography detection test paper strip of trichinellosis as well as a preparation method and application thereof and belongs to the technical field of detection of fluorescence immunodetection. In order to more rapidly, stably and accurately detect trichinization, the invention provides a fluorescence immunochromatography detection test paper strip oftrichinosis. The fluorescence immunochromatography detection test paper strip of trichinosis comprises a sample pad, a combination pad, a chromatography membrane, a water absorption pad and a substrate, wherein time-resolved fluorescence microsphere-coupled goat anti-swine IgG is labeled on the combination pad; a detection line and a quality control line are arranged on the chromatography membrane; a trichinella spiralis larvae ES mixed antigen composed of a trichinella spiralis larvae excretion and secretion material ML-ESP antigen and a trichinella intestinal phase spiralis larvae excretionand secretion material iML-ESP antigen is sprayed on the detection line; rabbit anti-goat IgG is sprayed on the quality control line; all the components are combined and prepared into the test paperstrip. The test paper strip can be applied to rapid detection of swine trichinellosis.

Description

technical field [0001] The invention belongs to the technical field of fluorescent immune detection, and in particular relates to a fluorescent immunochromatographic detection test strip for trichinosis and a preparation method and application thereof. Background technique [0002] Trichinellosis is a very serious zoonotic disease. It not only causes huge economic losses to animal husbandry production, but also poses a huge threat to human health. People or animals mainly contain trichinosis through raw or semi-raw food. Caterpillar meat (mainly pork) and disease. [0003] For the inspection of trichinellosis in slaughtered animals, the inspection methods of the regulations of the International Organization for Animal Health (OIE) are microscope inspection and sample digestion method, and these two methods are also being used in my country at present. However, the above two methods have certain disadvantages. Microscopic examination is time-consuming and laborious and has p...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/569G01N33/558G01N33/543G01N33/533G01N21/64
CPCG01N21/6428G01N33/533G01N33/54313G01N33/558G01N33/569G01N2021/6439
Inventor 刘明远刘晓雷杨勇王学林白雪唐斌丁静王楠张小波
Owner JILIN UNIV
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