The invention discloses a steak tenderness detection device, which comprises an operation table, the operation table is composed of a cutting table, a detection table and a cleaning table, the cleaning table is composed of a cleaning pool, a water inlet pipe, a water stop valve, a water outlet pipe and a water collecting pool, the cleaning pool is located on the left side of the operation table, and the detection table is located in the middle of the operation table. The detection table is composed of a drying machine, an ultraviolet lamp, a display screen and a detection system, the detectionsystem is composed of a telescopic support, a telescopic arm and a detection pen, the cutting table is located on the left side of the operation table, the cutting table is composed of a baffle, a knife sharpener, a knife, a sliding rod, a sleeve, a bolt, a knife handle, a buffer rod and a chopping board, the baffle is fixed on the upper surface of the cutting table, and a square notch is formedin the middle of the baffle, a sliding rod is fixed to the middle of the interior of the square notch, and telescopic legs are fixed to the positions, close to the edges, of the lower surface of the operation table. The device has the advantages of compact structure, convenient cleaning and accurate detection.