Preparation method and medical application of cervical carcinoma associated trichina antigen antibody

An antigen-antibody, cervical cancer technology, applied in the direction of antibodies, anti-tumor drugs, anti-animal/human immunoglobulins, etc., can solve the problem of unclear anti-tumor active ingredients, and achieve the effect of easy preparation and industrialization

Inactive Publication Date: 2011-11-16
JILIN UNIV
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Problems solved by technology

[0002] Trichinella spiralis is a nematode that parasitizes humans and various animals. In recent years, studies have found that it has strong anti-tumor activity, but it

Method used

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  • Preparation method and medical application of cervical carcinoma associated trichina antigen antibody

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Embodiment 1

[0030] Preparation of mouse anti-trichinella and cervical cancer Hela cell-associated antigen antibody:

[0031] The ELISA method was used to find that the soluble antigen of Trichinella spiralis could cross-react with the anti-Hela cell antibody, and preliminarily determined the antigen related to the immune cross-reaction between Trichinella spiralis and Hela cells. Then, determine the molecular weight of the antigen associated with Trichinella spiralis and Hela cells according to SDS-PAGE and Western (see attached figure 1 ), collect the 40KDa Trichinella spiralis and cervical cancer Hela cell-associated antigen protein, and measure the protein value of the collected Trichinella spiralis and cervical cancer Hela cell-associated antigen with a protein quantification kit. Immunize BalB / C mice after emulsification in Freund's adjuvant (subcutaneous immunization). After 10 days, after emulsifying with 100 μg antigen and the same amount of incomplete Freund's adjuvant, the sec...

Embodiment 2

[0033] Mouse anti-Hela cells and trichinella-related antigen antibody serum preparation:

[0034] The ELISA method was used to find that the soluble antigen of Hela cells could cross-react with the anti-T. Then, according to SDS-PAGE and Western to determine the molecular weight of the antigen associated with Hela cells and Trichinella spiralis (see attached figure 1 ), collect Hela cells and the 55KDa related protein of the Trichinella spiralis-associated antigen, quantify the obtained Hela cell antigen protein, emulsify 100 μg of the antigen with the same amount of complete Freund’s adjuvant, and immunize the BalB / C small mice (subcutaneous immunization). After 10 days, after emulsifying with 100 μg antigen and the same amount of incomplete Freund's adjuvant, the second immunization was also carried out, and the third immunization was carried out one week later. After the second and third immunizations, blood was collected from the tail vein of the mice to measure the pot...

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Abstract

The invention provides a preparation method of a cervical carcinoma cell associated trichina antigen antibody, and also provides medical application of the antibody. Tumor cells are relatively sensitive to a foreign identifier, the cervical carcinoma cell associated trichina antigen can generate strong specific and nonspecific immune reactions on tumor, and simultaneously, the tumor cell associated trichina antigen is easy to prepare and industrialize.

Description

technical field [0001] The invention provides a preparation method of an antigen antibody related to trichinella spiralis and cervical cancer cells, and also provides a medical application of the antibody, which belongs to the field of biopharmaceuticals. Background technique [0002] Trichinella spiralis is a nematode that parasitizes humans and various animals. Recent studies have found that it has strong anti-tumor activity, but its anti-tumor active ingredients are still unclear. In particular, there is no literature report on the antigen antibody associated with Trichinella spiralis and cervical cancer. Contents of the invention [0003] The invention provides a preparation method of an antigen antibody related to trichinella spiralis and cervical cancer cells, which is suitable for industrial production. [0004] The invention discloses the medical use of antigen antibody related to trichinella spiralis and cervical cancer cells, which is used for preparing a pharma...

Claims

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Application Information

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IPC IPC(8): C07K14/435C07K16/18A61K39/395A61P35/00
Inventor 张西臣张国才王燕春李建华宫鹏涛杨举李赫张楠李淑红
Owner JILIN UNIV
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