Toxoplasma circulating antigen double antibody sandwich ELISA (Enzyme-Linked Immuno Sorbent Assay) detection method

A double anti-sandwich, Toxoplasma gondii technology, applied in chemical instruments and methods, botanical equipment and methods, biochemical equipment and methods, etc., can solve problems such as false negatives, inability to determine current infection or past infection, and low immune function.
CN102010468AInactive Publication Date: 2011-04-13JILIN UNIV
0 Cites 3 Cited by

Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
JILIN UNIV
Publication Date
2011-04-13
Estimated Expiration
Not applicable · inactive patent

Smart Images

  • Figure 1
    Figure 1
  • Figure 2
    Figure 2
  • Figure 3
    Figure 3
Patent Text Reader

Abstract

The invention discloses a toxoplasma circulating antigen double antibody sandwich ELISA (Enzyme-Linked Immuno Sorbent Assay) detection reagent and a preparation method. Toxoplasma MIC10 is a protein secreted by toxoplasma when invading a host cell and has important actions in invasion and proliferation of the toxoplasma. In the invention, amplification and expression at different areas are carried out on an MIC10 gene to obtain recombinant fusion proteins MIC10A, MIC10B and MIC10C, the purified recombinant fusion proteins MIC10A and MIC10B respectively immunize rabbits, are used for preparinga polyclonal antibody and are respectively used as a coated antibody and a detection antibody, a double antibody sandwich ELISA is established with purified MIC10C as a standard antibody to detect a toxoplasma circulating antigen in serum and has the advantages of strong specificity, strong sensitivity, strong reproducibility and strong stability, wherein y is equal to 0.0304Ln(x)-0.0567, R2 is equal to 0.9643, and the minimum detectable amount is 50pg / ml.
Need to check novelty before this filing date? Find Prior Art

Description

technical field

[0001] The invention belongs to the technical field of biological inspection and quarantine. Specifically, a recombinant protein is constructed, and antibodies prepared by immunizing the recombinant protein are used to establish a double-antibody sandwich ELISA detection method for circulating antigens of toxoplasma gondii. Background technique

[0002] Toxoplasma gondii (toxoplasma) is an obligate intracellular parasitic protozoan, which has a wide range of host groups and is prevalent worldwide, causing zoonotic parasitic diseases. Toxoplasma gondii, as an opportunistic infection factor, can cause death in patients with impaired or suppressed immune function such as AIDS patients, organ transplantation patients and malignant tumor patients. Toxoplasmosis is not only an important disease in medicine, but more importantly, it is an important biological factor affecting human prenatal and postnatal care.

[0003] The human body is infected with Toxoplasma gon...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More