Hybridoma cell line and monoclonal antibody for producing angiostrongylus cantonensis V-stage larvae 55KD antigen

Abstract

The invention discloses a hybridoma cell line ACV1 and a monoclonal antibody ACV McAb for producing angiostrongylus cantonensis V-stage larvae 55KD antigen. The antibody and the angiostrongylus cantonensis V-stage larvae 55KD antigen can be in specific binding. The preparation method of the antibody is simple, and the antibody can be obtained by direct secretion of the hybridoma cell line ACV1. The antibody has higher binding specificity and affinity with the angiostrongylus cantonensis V-stage larvae 55KD antigen, and the monoclonal antibody can be applied to detect the angiostrongylus cantonensis circulating antigen levels, thereby providing a method of early diagnosis of the angiostrongylus cantonensis.

Description

technical field [0001] The invention relates to a hybridoma cell capable of producing monoclonal antibody against 55KD antigen of angiostrongylus cantonensis V stage larvae and a method for producing the anti-55KD antigen monoclonal antibody of angiostrongylus cantonensis V stage larvae. Background technique [0002] Angiostrongyliasis cantonensis refers to eosinophilic meningitis or meningoencephalitis (Eosinophilic meningitis) mainly manifested by acute severe headache caused by Angiostrongyliasis cantonensis larvae migrating in the human body and invading the central nervous system. or Meningoencephalitis, EM). Since the first case reported in Taiwan in 1945, the disease has been reported successively in mainland China and showed an increasing trend year by year. Subsequently, collective small-scale outbreaks occurred in Wenzhou and Fujian respectively, and the incidence rate reached 44.8%. In 2003, Angiostrongylus cantonensis was listed as an emerging infectious disease...

AI Technical Summary

Problems solved by technology

In 2003, Angiostrongylus cantonensis was listed as an emerging infectious disease by the Ministry of Health, but it did not attract enough attention, lacking a systematic understanding of the disease, efficient detection methods, and standardized diagnosis and treatment measures

However, the diagnosis of the disease in China is still at the stage of antibody detection, because of the following problems: ①The antibody appears late after infection, ②The antibody will still exist in the body for a long time after complete cure, so it is impossible to diagnose and diagnose the current infection. Efficacy evaluation, ③ High cross-reaction with other helminth infections, etc., which limits the reliability of these immunodiagnostic methods

Images