194results about How to "Facilitate early diagnosis" patented technology

A method, system and computer readable medium for automated detection of lung nodules in computed tomography (CT) image scans, including generating two-dimensional segmented lung images by segmenting a plurality of two-dimensional CT image sections derived from the CT image scans; generating three-dimensional segmented lung volume images by combining the two-dimensional segmented lung images; determining three-dimensional lung nodule candidates from the three-dimensional segmented lung volume images, including, identifying structures within the three-dimensional segmented lung volume images that meet a volume criterion; deriving features from the lung nodule candidates; and detecting lung nodules by analyzing the features to eliminate false-positive nodule candidates from the nodule candidates.

The invention discloses an Alzheimer's disease classification method based on a depth neural network and multi-mode images, and the method comprises the steps: taking the 2D and 3D forms of the images of N classes of Alzheimer's diseases as the input layer, taking the probability of a classification result as the output layer, and constructing a depth neural network; carrying out the preprocessing of the images of N known classes, and obtaining a training sample; training the depth neural network through the training sample, optimizing the weight of network connection, and obtaining a final depth neural network; inputting the preprocessed to-be-classified image into the final depth neural network, and outputting a classification result. In order to make the most of the feature information of the brain of an AD patient, the method introduces the multi-mode image information: an MRI image, a PET image and a DTI image on the basis of a conventional single-mode medical image classification method, and the method achieves the fusion of non-image feature CSF information and gene information.

The invention discloses a nucleic acid analyzing method based on cascade HCR (hybridization chain reaction), and belongs to the field of molecule detection. The nucleic acid analyzing method is characterized in that on the basis of single HCR, two stages of HCR reaction are designed, and the signals can be further amplified on the basis of single HCR; the hairpin nucleic acid probes marked with multiple different fluorescent dyes can be alternatively hybridized by a final target product, so as to form a branched DNA (deoxyribonucleic acid) nanometer structure; the signal output is provided bythe fluorescent resonance energy transfer, and the concentration of the target nucleic acid can be judged according to the change value of the fluorescence intensity of fluorophore. The nucleic acid analyzing method has the advantages that the nucleic acid analyzing method can be used for in-vitro detection of DNA and miRNA (miniature ribonucleic acid), the specificity is good, and the sensitivityis high; the nucleic acid analyzing method can be used for the imaging analysis of miRNA in cells, and be favorable for the early diagnosis on cancers.

The invention relates to a reflecting photometer of golden mark immunity test tape that includes scanning platform, optical system, phototranslating and signal multiplication system and data gathering and control system. The scanning platform is used to locate tested golden mark immunity test tape, and the optical system includes plural illuminating optical paths and a receiving optical path. Each illuminating optical path contains a light source and an illuminating condensing lens. The receiving optical path is made up of collimating mirror, filter, condensing lens and slit diaphragm. The phototranslating and signal multiplication system is made up of phototranslating components and pre-amplification circuit, the data gathering and control system is made up of multifunction data gathering card and computer. The invention could accurately judge the tested object and the thickness. It also has the advantages of high sensitivity, objective testing result and flexible to use.

The invention relates to the technical field of clinic kits and particularly relates to a method for detecting immunological turbidimetry of hypersensitive cardiac troponin I magnetic microspheres and a detection kit. The kit comprises a reaction diluent reagent, a cTnI antibody-coupled magnetic microsphere reagent and a cTnI correction product. Compared with a detection result of an existing cTnI chemiluminiscence detection kit, the method and the detection kit have the advantages that by carrying out statistic analysis, the relativity is good, no remarkable difference exists, the kit can be clinically popularized and used, and a relatively good choice is provided for the detection of cardiac troponin I.

The invention provides an immunity diagnosis test kit for detecting II-type dengue virus antigen, which comprises a porous reaction plate covering monoclonal antibody DV2-M6, a sample treatment liquid, a monoclonal antibody DV2-M15 marked with a label, a positive contrast, a negative contrast, a concentration washing liquid, a develop liquid and a termination liquid, wherein the monoclonal antibodies DV2-M6 and DV2-M14 of the test kit can be specifically combined with NS1 protein of II-type dengue virus, without cross reaction with other three kinds of serotype dengue viruses NS1 and respectively combined with different antigen points of NS1, while the check sensitivity of NS1 protein of II-type dengue virus can reach 3ng/ml and the check sensitivity of culture supernatant of II-type dengue virus infection cell is 8 power of Pan-E dengue early elisa test kit, thereby improving the sensitivity of clinical serum sample check.

The invention relates to a mimic electrochemical immunosensor for detecting beta-amyloid protein (ABeta) oligomers and preparation method thereof and belongs to the technical field of biosensing and electroanalytical chemistry. gold nanoparticles jointly act with sulfhydryl-modified aptamer and thionine to form a stable nano conjugate; an antibody is fixed to the surface of a carboxylic graphene oxide modified electrode; after an ABeta oligomer is recognized, the oligomer is further connected with the aptamer modified gold nanoparticle conjugate, thus forming a sandwich mimic immunosensor; the ABeta oligomer is measured through thionine electrochemical signals. The immunosensor has high sensitivity, good specificity and high stability; compared with traditional immunosensors, the immunosensor features preparation simplicity and avoidance of the use of enzyme-labeled antibody reagents, is useful in the detection of ABeta oligomers in cerebrospinal fluid to promote early diagnosis of Alzheimer's disease and has clinical applicable value.

The present invention is directed to new ways to diagnosis lung cancer, especially at an early clinical stage. In addition, prognosis and the monitoring of therapeutic agents or other treatments, for lung cancer patients, can be accomplished with the disclosed methods. The methods also find use in allowing the assessment by pre-clinical animal efficacy studies to screen for the useful of therapeutic agents for treating lung cancer.

The invention belongs to the fields of genetic engineering and reproductive medicine and relates to a serum/plasma miRNA (micro Ribonucleic Acid) marker relevant to gestational diabetes and application of the serum/plasma miRNA marker. The marker is a combination of miR-132, miR-29a and miR-222. The marker and a prime thereof can be used for preparing a diagnosis kit and used for auxiliary early-stage diagnosis of the gestational diabetes.

The invention is about the PCR detecting method of a respiratory tract pathogen and the reagent box. The character is to design the vector and the probe according to the highly special base groups of the pathogen by analyzing the nucleic acid sequence of the pathogen. So next to amplify the PCR or the RT-PCR in the fluorescent PCR instrument.

The detection process of SARS virus of the gene detection reagent kit with rationing standard specific SARS virus nucleic acid as reference includes RNA extraction, PCR amplification and fluorescent detection. The venous blood sample, gargled liquid or respiratory tract secretion of the patient is used as the analysis sample directly, RNA of the sample is extracted as the nucleic acid template, and the template is used in fluorescent PCR amplification. The used fluorescent amplification detecting reagents includes one-step process RT-PCR buffering liquid, deoxynucleoside triphosphate (dNTP) mixture, specific amplification primer and specific probe. The method of the present invention is fast and convenient in detecting SARS virus.

The invention discloses an immunodiagnosis kit for detecting specific IV-type dengue virus antigen, comprising a microporous reaction plate coated with monoclonal antibody 2E8A5, sample treatment fluid, monoclonal antibody 7A6A1 combined with markers, positive control, negative control, wash concentrate, color developing solution and stop solution. The kit can detect IV-type dengue virus antigen specifically, does not cross-react with other three types of serotype dengue virus antigens and detects that the supernatant sensitivity of the IV-type dengue virus is 64 times of that of the commercial kit of the same type, namely, the Pan-E Dengue Early ELISA test kit, thus greatly improving the sensitivity of detection of clinical serum samples and simultaneously reducing omission rate in clinical applications.

The invention belongs to the gene field of biotechnology, and relates to a single-nucleotide polymorphism (SNP) site rs9275319 related to liver cancer susceptibility and an application thereof. The invention provides a gene partial sequence (the sequence is shown by SEQ ID No.1) of the genetic area 6p21.3 and an SNP site rs9275319 (the sequence is shown by SEQ ID No.2). The invention also provides a method for detecting the SNP site, a specific nucleic acid primer with sequences shown by SEQ ID No.3 and SEQ ID No.4, and a UEP primer with a sequence shown by SEQ ID No.5. The invention also relates to a detection kit for the SNP site rs9275319 and an application thereof. In the invention, genotyping can be performed on the rs9275319 site, the method is simple and quick, the result is accurate and clear, and a new means is provided for the liver cancer susceptibility identification as well as prevention and treatment.

A molecular diagnosing method for botrytis of tomato includes such steps as designing a pair of PCR primers specific to the pathogen of tomato botrytis, using them for PCR amplifying of the specimen to be tested, and judging if the tomato plant is suffered from botrytis. Its advantages are high speed, high sensitivity and high specificity.

A protein chip for diagnosing the early-phase malignant tumor is prepared by arraying the label antigens of different tumors, positive references (the marker of relative tumor) and negative references (human serum albumin) in a same cavity of protein chip by full-automatic high-speed robot, and the protein is linked to the solid carrier by chemical bonds. Its advantages are high sensitivity and specificity, high stability and repeatability of result, high correctness and efficiency.

The invention relates to a protein chip, a protein chip diagnostic kit, a preparation method and a using method. The protein chip contains the following protein markers of autologous antigen protein: a P53 antigen fragment, an SOX2 antigen fragment, a COPB1 antigen fragment, an EFHD2 antigen fragment, an EIF4G3 antigen fragment and a PCNA antigen fragment, wherein the characteristic amino acid sequence of the P53 antigen fragment is as shown in ID.1sequence; the characteristic amino acid sequence of the SOX2 antigen fragment is as shown in ID.2sequence; the characteristic amino acid sequence of the COPB1 antigen fragment is as shown in ID.3sequence; the characteristic amino acid sequence of the EFHD2 fragment is as shown in picture 4seeqeunce; the characteristic amino acid sequence of the EIF4G3 antigen fragment is as shown in ID.5seqeunce; the characteristic amino acid sequence of the PCNA antigen fragment is as shown in ID.6sequence. Early diagnosis or general investigation screening for suspected patients of lung cancer or high-risk population of lung cancer can be carried out extremely conveniently, quickly, noninvasively and efficiently.

The invention belongs to the field of biological detection and specifically relates to a triple test diagnostic kit for breast cancer of tumor serum markers CA125, HE4 and P-her2. The triple test diagnostic kit for breast cancer provided by the invention mainly comprises an abzyme elisa plate, a biotin antibody enveloped plate, bovine serum albumin, serum diluent, horse radish peroxidase, tetramethyl benzidine, sulfuric acid, negative control liquid and positive control liquid. The triple test diagnostic kit for breast cancer provided by the invention can be used for detecting the expression levels of CA125, HE4 and P-her2 in the body of the patient after operative treatment or the relapsing and metastatic tumor patient; the triple test diagnostic kit for breast cancer has important significance in clinical diagnosis of breast cancer; the triple test diagnostic kit for breast cancer provided by the invention can reduce the interference of other matters in serum, can greatly increase the accuracy and stability of detection result and is beneficial to the early diagnosis and prognosis treatment of the patient suffering from breast cancer.

The (Streptococcus suis Serotype 2 (SS2) virulence gene PCR amplification kit includes a SS2DNA contrast template, a PCR amplification detecting reagent, an agarose gel electrophoresis reagent and a DNA gradient standard. The PCR amplification detecting process includes the measurement of 7 important SS2 genes, diagnosing Streptococcus infection and SS2 infection based on whether to amplify specific Streptococcus gene, specific SS2 gene and 5 relevant important SS2 virulence genes, and predicting the virulence, invasiveness trend and disease prognosis fast and early. The present invention is favorable to the clinical diagnosis of borderline case and early warning, and is hopeful to be applied in emergency detection of human SS2 infection, etc.

The invention discloses a primer and probe sequence used for LAMP-LFD detection of vibrio fluvialis and application of the primer and probe sequence. The primer and probe sequence is characterized by comprising three pairs of primers of LAMP including VflompU-F3 and VflompU-B3, VflompU-FIP and VflompU-BIP and VflompU-LF and VflompU-LB and a probe VflompU-HP, and a nucleotide sequence is shown as SEQ NO1-NO7. The primers and the probe are utilized to realize visual detection of vibrio fluvialis through a step of amplification by an LAMP reaction system, a step of enabling the probe to be hybridized with an LAMP reaction product and a step of LFD detection. The primer and probe sequence has the advantages of higher quickness, specificity and sensitivity, simplicity in instrument need, conduciveness to early diagnosis and detection of vibrio fluvialis and capability of meeting detection needs of primary detection mechanisms and on-site epidemic focuses.

The invention discloses a detecting device for Brucella infection. The detecting device comprises a test strip; a Brucella antigen detection area and a Brucella antibody detection area are arranged on a chromatographic membrane of the test strip. According to the detection device, a Brucella antigen and a Brucella antibody in a sample can be detected simultaneously; various outer membrane proteins are applied in a combined manner, so that the occurrence of false positive results can be reduced effectively; a double-antigen sandwich method and a double-antibody sandwich method are adopted, so that the sensitivity and the specificity of detection can be higher than those obtained according to the conventional indirect method, and the rapid detection of brucellosis is realized; the problems of high interference, and high false positive result or false negative result occurrence rate caused by long detection period, poor specificity and low sensitivity in the conventional brucellosis detection process are solved.

The invention discloses a preparation method and application of magnetic nanoparticles for capturing exosomes in blood. The preparation method is characterized by comprising the following steps: (1) after mixing diferric chloride hexahydrate and ferrous chloride tetrahydrate, dissolving a mixture into distilled water; after stirring and uniformly mixing, heating to 70 DEG C; then slowly dropwise adding an ammonia water solution; after hydrolysis is basically finished, slowly adding cetyl trimethyl ammonium bromide into the solution; stirring, centrifuging and collecting a product and washing;taking lower-layer sediment and drying in vacuum to obtain nano Fe3O4 particles; (2) after dispersing the nano Fe3O4 particles into an ethanol water solution and carrying out ultrasonic treatment, adding a tetraethyl orthosilicate solution; gradually adding into an ammonia water bath and reacting for 5 h; centrifuging and collecting a product; washing and drying to obtain the irregular magnetic micro-nanoparticles which are covered with SiO2 and have the surface distributed pore diameter of 40 to 200 nm. The preparation method disclosed by the invention has the advantages that the exosomes arelosslessly combined to the greatest extent, the amount of used samples is small, the treatment time is short and the operation is simple and convenient.

The invention discloses a bone fault image reconstruction method and system. The method comprises the following steps of S1, obtaining a three-dimensional bone CT image template; S2, generating a local two-dimensional bone gamma plain film image template; S3, matching the whole body bone gamma plain film image to obtain the most matched local two-dimensional bone gamma plain film image; S4, further optimizing the three-dimensional bone CT image template, and enabling the two-dimensional bone gamma image template corresponding to the three-dimensional bone CT image template to completely cover the region of interest of the local bone gamma image; s5, reconstructing a quantitative local bone gamma tomography image based on the template; and S6, carrying out fusion display on the local bone gamma tomography image and the CT tomography image. By means of the method and system, the level close to the quality of a tomographic image obtained through conventional SPECT tomographic acquisition and reconstruction can be achieved after fusion, a doctor can analyze and diagnose the tomographic image more visually, clinical examination is conducted on part of patients, extra tomographic acquisition is avoided, and efficiency is improved.

The invention discloses a targeted polypeptide for vitro and vivo early diagnosis or treatment of lung cancer, belonging to the medicine technical field. The polypeptide is dodecapeptide. The invention also discloses the application of the targeted polypeptide. The targeted polypeptide is applied to a carrier of the targeted therapy of the lung cancer or the preparation of anti-cancer drugs, or used as the medicine of early period and the middle period diagnosis of the lung cancer. The polypeptide provided by the invention is small micro molecule antibody and has smaller antibody molecular weight and strong penetrating power, is easy to reach tumor tissues and can be more sensitive to diagnose the tumor apart from having the advantages of the traditional antibody medicine, thus being in favor of the early diagnosis of the tumor. The polypeptide has good targeted function of the tumor, is suitable for the targeted treatment or the carrier of the targeted treatment and has wide application prospect.

The invention discloses a kit for detecting TORCH IgM antibodies. The kit comprises a solid carrier which is coated with an antibody combined with the TORCH IgM antibodies, a TORCH antigen which is marked with lanthanide, a TORCH IgM antibody calibrator, a sample diluent, an experiment buffering solution, a concentration cleaning solution and an enhancement solution. The kit can simultaneously detect multiple pathogene IgM antibodies in TORCH pathogene under the same detection condition and is high in sensitivity, high in specificity, good in precision, high in accuracy, little in blood consumption amount, rapid, convenient and favorable for early diagnosis on the infection of the TORCH pathogene. The method has the characteristic that the detection linear range is wide, the diluting times or diluting ratio of a high-value sample can be reduced, and the accuracy of a detection result can be improved.

The invention provides a fluorescent quantitative kit for fast detecting SNP loci. The fluorescent quantitative kit comprises reaction mixed liquid and an operation instruction and is characterized inthat the reaction mixed liquid comprises first primers, second primers, third primers, a nucleotide monomer mixture, endoribonuclease, nucleic acid polymerase and nucleic acid double-strand fluorescent dye, the first primers are used for identifying the SNP loci, the second primers and the third primers are used for assisting the first primers to perform SNP characteristic sequence isothermal amplification, and the reaction system adopts a primer activated isothermal strand displacement nucleic acid amplification manner. The fluorescent quantitative kit for fast detecting the SNP loci has theadvantages that the kit is high in sensitivity, capable of detecting the target object of 22aM and good in selectivity and has mispairing distinguish rate of 10000 times, the reaction is completed inone step and is a closed-tube reaction, cross contamination is avoided, reaction time is short, fluorescent labeling is not needed, genome samples can be directly detected, and time and manpower costare saved.