Targeted polypeptide for diagnosing and treating lung cancer in vivo and in vitro and use thereof

A targeted, lung cancer technology, applied in the field of medicine, can solve the problems of lack of specificity and sensitivity, and achieve the effect of small molecular weight, strong penetrating power, and sensitive diagnosis of tumors

Inactive Publication Date: 2009-08-12
GUANGDONG PHARMA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the diagnostic methods used in clinical practice are mainly traditional chest radiography, bronchoscopy, sputum cytology, etc., which lack sufficient specificity and sensitivity. Most lung cancer patients are diagnosed at an advanced stage.

Method used

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  • Targeted polypeptide for diagnosing and treating lung cancer in vivo and in vitro and use thereof
  • Targeted polypeptide for diagnosing and treating lung cancer in vivo and in vitro and use thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] The invention is obtained by using the phage display technology in vivo to screen the polypeptide specifically bound to lung cancer tissue, and identifying its specificity and affinity, and has good lung cancer targeting specificity.

[0029] Reagent Phage Dodecapeptide Library (Ph.D-12 TM Phage Display Peptide Library) was purchased from NewEngland Biolabs, and the library titer was 1.5×10 13 pfu / ml with a complexity of 2.7 × 10 9 ; Receptor bacterium E.coliER2738 was purchased from New England Biolbs; HRP / anti-M13antibody (#27-9421-01, 1:5000 diluted working concentration) was purchased from Amersham Biosciences; TMB, IPTG, Xgal and other reagents were all Purchased from Beijing Dingguo Company.

[0030] Instrument CO 2 Incubator, inverted microscope, biological safety cabinet, high-speed refrigerated centrifuge, microplate reader,.

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PUM

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Abstract

The invention discloses a targeted polypeptide for vitro and vivo early diagnosis or treatment of lung cancer, belonging to the medicine technical field. The polypeptide is dodecapeptide. The invention also discloses the application of the targeted polypeptide. The targeted polypeptide is applied to a carrier of the targeted therapy of the lung cancer or the preparation of anti-cancer drugs, or used as the medicine of early period and the middle period diagnosis of the lung cancer. The polypeptide provided by the invention is small micro molecule antibody and has smaller antibody molecular weight and strong penetrating power, is easy to reach tumor tissues and can be more sensitive to diagnose the tumor apart from having the advantages of the traditional antibody medicine, thus being in favor of the early diagnosis of the tumor. The polypeptide has good targeted function of the tumor, is suitable for the targeted treatment or the carrier of the targeted treatment and has wide application prospect.

Description

technical field [0001] The invention relates to a targeting polypeptide and its application, in particular to a targeting polypeptide for in vivo and in vitro diagnosis or treatment of lung cancer and its application, which belongs to the field of medical technology. Background technique [0002] Lung cancer is a common malignant tumor. According to the latest statistics in the United States, the incidence of lung cancer in 2007 was second only to prostate cancer in men and breast cancer in women, but the death rate ranked first among all malignant tumors. The sensitivity of lung cancer to radiotherapy and chemotherapy is poor, and the treatment options are limited. Therefore, the mortality rate of lung cancer is extremely high, and the average 5-year survival rate is less than 10%. Selectively blocking the signal transduction pathway that leads to unlimited proliferation of tumor cells, modifying or blocking a certain (some) pathogenic gene or gene site, and stimulating and...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K16/18A61K39/395A61K47/42A61P35/00G01N33/574
Inventor 臧林泉潘琴石磊张德志冯冰虹潘雪刁
Owner GUANGDONG PHARMA UNIV
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