MCI diagnosis marker due to AD and application of MCI diagnosis marker

A diagnostic marker, marker technology, applied in the field of diagnostic markers, the prodromal stage of Alzheimer's disease

Active Publication Date: 2018-12-21
上海益信佳生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Binding of hsa-miR to the 3'UTR of AD target genes limits post-transcriptional expression by limiting translation initiation or promoting target gene cleavage

Method used

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  • MCI diagnosis marker due to AD and application of MCI diagnosis marker
  • MCI diagnosis marker due to AD and application of MCI diagnosis marker
  • MCI diagnosis marker due to AD and application of MCI diagnosis marker

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Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0028] In order to describe the technical content of the present invention more clearly, further description will be given below in conjunction with specific embodiments.

[0029] combine Figure 1~3 , the screening, verification, etc. of the AD-induced MCI diagnostic markers provided by the present invention will be described in detail.

[0030] 1. Research object

[0031] The subjects were 50 elderly cases of MCI caused by AD collected in the communities of Changning District and Xuhui District, Shanghai. The control group was healthy elderly people matched in age, sex and years of education.

[0032] 2. Research methods

[0033] 1. Chip screening

[0034] (1) RNA was extracted by TRIzol method, and purified by RNasey Mini Kit (QIAGEN). Use NanoDropND-1000 to measure the concentration of purified RNA, and electrophoresis to detect RNA integrity.

[0035] (2) After the extracted RNA passed the quality inspection, use the miRCURYTM Array Power Labeling kit (Cat#208032-A, ...

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Abstract

The invention relates to an MCI diagnosis marker due to AD (Alzheimer 's disease). The marker is plasma miRNA which comprises hsa-miR-1185-2-3p, hsa-miR-22-5p, hsa-miR-134-3p, hsa-miR-1909-3p and hsa-miR-107. The invention further relates to application of the marker and a corresponding reagent kit. The MCI diagnosis marker has the beneficial effects that a biology marker having high diagnosis value for MCI due to AD is found for the first time, the difficult situation that a convenient periphery plasma diagnosis marker for MCI due to AD does not exist is broken through, and early diagnosis and early intervention of Alzheimer 's disease commonly called senile dementia are facilitated.

Description

technical field [0001] The present invention relates to the technical field of prodromal stage of Alzheimer's disease, in particular to the technical field of diagnostic markers, in particular to a diagnostic marker for MCI caused by AD and its application. Background technique [0002] Alzheimer's disease (AD), commonly known as senile dementia, is a neurofibrillary tangle formed by the deposition of amyloidβ-protein (Aβ) in the brain and the disorder of microtubule structure caused by hyperphosphorylated Tau protein. Neurodegenerative disease as the main pathological feature. AD has become a common, frequently-occurring and high-burden disease in the elderly. AD is a chronic, non-infectious, age-related neurodegenerative disease of complex etiology. In the more than 100 years since the German psychiatrist Alzheimer first reported the disease in 1906, the disease has become the most common type of senile dementia from a rare or uncommon disease, accounting for about 50% t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6883C12N15/113
CPCC12Q1/6883C12Q2600/178C12Q2600/158C12Q2600/166C12Q1/686
Inventor 王涛肖世富许桦何海宁杨寰庆
Owner 上海益信佳生物科技有限公司
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