Method and kit for detecting TORCH IgM antibodies and preparation method of kit

A kit and antibody technology, applied in the field of detection of TORCH IgM antibody based on multi-label time-resolved fluorescence immunoassay, can solve the problems of great influence of the environment and operators, experimental errors, and increased blood collection volume, so as to reduce sample consumption and detection. Errors, the effect of reducing workload
CN104155449AInactive Publication Date: 2014-11-19GUANGZHOU FENGHUA BIOENG
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
GUANGZHOU FENGHUA BIOENG
Publication Date
2014-11-19
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses a kit for detecting TORCH IgM antibodies. The kit comprises a solid carrier which is coated with an antibody combined with the TORCH IgM antibodies, a TORCH antigen which is marked with lanthanide, a TORCH IgM antibody calibrator, a sample diluent, an experiment buffering solution, a concentration cleaning solution and an enhancement solution. The kit can simultaneously detect multiple pathogene IgM antibodies in TORCH pathogene under the same detection condition and is high in sensitivity, high in specificity, good in precision, high in accuracy, little in blood consumption amount, rapid, convenient and favorable for early diagnosis on the infection of the TORCH pathogene. The method has the characteristic that the detection linear range is wide, the diluting times or diluting ratio of a high-value sample can be reduced, and the accuracy of a detection result can be improved.
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Description

technical field

[0001] The invention relates to a method and a kit for TORCH IgM antibodies, in particular to a method for detecting TORCH IgM antibodies based on multiple label time-resolved fluorescence immunoassay, a kit and a preparation method for the kit. Background technique

[0002] The term TORCH was formed by the American scholar Nahmias in 1971 by combining the first letters of the English nouns of pathogens that would cause infection of embryos (fetus) in the uterus, cause abortion, or even cause birth defects or abnormal development after several pregnant women became ill. Refers to a group of pathogens: T is Toxoplasma gondii; O is others, such as hepatitis B virus, HIV virus, Treponema pallidum, etc.; R is rubella virus (Rubellavirus); C is cytomegalovirus (Cytomegalovirus); H stands for herpes simplex virus.

[0003] The above four pathogens have the following points in common: 1. They can spread vertically through the placenta and cause intrauterine infecti...

Claims

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