The invention discloses a four-color TORCH (Toxo, others, RV, CMV and HSV) fluorescent detection kit, comprising a hot start Taq enzyme system, primers, a fluorescent probe, a negative control and a positive control; the fluorescent probe is a TORCH-specific MGB (minor groove binder) probe. The four-color TORCH fluorescent detection kit is suitable for qualitatively detecting toxoplasma, rubella virus, cytomegalovirus, and herpes simplex virus type I and type II in serum and plasma samples. The four-color TORCH fluorescent detection kit detects toxoplasma, rubella virus, cytomegalovirus, and herpes simplex virus type I and type II in a same tube amplification system by means of multiple fluorescence PCR (polymerase chain reaction) technique. The four-color TORCH fluorescent detection kit has high detection flux and has greatly reduced screening cost.