55 results about "Measles virus IgG" patented technology

The present invention provides methods and compositions to induce neutralizing antibodies in mammals to serotypes of dengue virus, measles virus, mumps virus, rubella and/or VZV virus.

The invention relates to a gene chip for detecting important respiratory pathogenic viruses. A preparation method of the gene chip comprises the following steps of: preparing a specific primer; preparing a virus specific probe; preparing an oligonucleotide chip; establishing an RT-PCR (Reverse Transcription-Polymerase Chain Reaction) system; establishing a hybrid system; preparing a visual detection reagent; and establishing a developing method. The gene chip prepared by the invention can be used for simultaneously discriminating nine general respiratory pathogenic viruses comprising A and B type influenza viruses, parainfluenza viruses type 1 and 2, human metapneumovirus, respiratory syncytial virus, measles virus, rubella virus and mumps virus, provides a new solution for quickly detecting the general respiratory pathogenic viruses at high throughout and can provide guidance for monitoring, clinically diagnosing and treating the respiratory pathogenic viruses.

The invention relates to a preparation method of a human measles immune sensor based on graphdiyne and a silver nanoparticle cage, and belongs to the technical field of novel biological sensing and detection. Graphdiyne is of a carbon-carbon triple-bond linear structure with an sp hybridized state and has the advantages of zero cis-trans-isomer, high conjugation and the like; due to excellent electrical, optical and photoelectric properties, graphdiyne is a key material of a novel electronic and photoelectric device of the next generation. Meanwhile, by the use of good pi-pi conjugation effect, a high-flux load of signal molecules is realized, and a signal error caused by instable combination is reduced. Therefore, the human measles immune sensor based on graphdiyne and the silver nanoparticle cage can sensitively detect measles viruses.

The invention discloses a one-step real-time fluorescence quantitative RT-PCR (reverse transcription-polymerase chain reaction) kit for measles virus, relating to the PCR kits in the biotechnology field. The kit comprises a pair of specific primers, a specific fluorescent probe, positive control, negative control, one-step 5*RT-PCR buffer and Enzyme Mix of the measles virus, wherein the specific primers are F:5'-ACASRGTGATCAAARTGRRARYGAGCTC-3' and R:5'-GYCCTGCCATGGYYTGCAC-3'; and the specific probe is FP: FAM-ATCYGATRCAGTRTCAATT-MGB-NQF. The kit has the following advantages and positive effects: the detection time period is short and the detection efficiency is high; the specificity and accuracy rate in detecting the virus are high; quantitative analysis of the virus can be carried out while carrying out qualitative analysis of the virus; the detection sensitivity is higher than the sensitivity in the common PCR and immunological detection methods; the kit is easy to operate and popularize; and the experimental results have good repeatability.

The present invention provides coding sequences and structures of all the non-coding regions and P and M coding regions of Longchun-47 measles virus, protein amino acid sequence and structure of P and M coding regions, the use of the non-coding region sequences in constituting the vaccine virus carrier and the constituting process of the carrier. These data and method may be used in recombining multiple effect vaccine or gene treatment via utilizing the vaccine strain virus to constitute expression vector and may be also used in molecular biological diagnosis and reagent development for measles virus infection.

The invention discloses a system and a method for rescuing measles viruses and recombinant measles viruses for expressing GFP (Green Fluorescent Protein) and application. The system provided by the invention comprises the following components: 1) a full-length transcription plasmid pT7MVS191 or pT7MVS191-ATU-GFP containing a measles virus anti-genome; 2) a helper plasmid 1 containing an encoding gene of nucleocapsid protein of the measles viruses; 3) a helper plasmid 2 containing an encoding gene of phosphoprotein of the measles viruses; and 4) a helper plasmid 3 containing an encoding gene of RNA (Ribonucleic Acid) polymerase of the measles viruses. The invention further provides a method for rescuing the measles viruses and the recombinant measles viruses. By adopting the system and the method, the measles viruses and the recombinant measles viruses can be effectively rescued; and the system and the method can be used for developing and applying therapeutic or preventive recombinant measles virus products.

The invention provides a construction method and application of an F gene replaced chimeric measles attenuated strain. Specifically, the invention provides the chimeric measles virus attenuated strain, and the attenuated strain is a measles virus rMV/F (H1a) with the preservation number of CCTCCNO: V202101. The invention also provides a vaccine composition containing the F gene substituted chimeric measles attenuated strain or the derivative virus strain thereof as an active ingredient and a preparation method of the vaccine composition. Compared with the existing measles vaccine strain in the market, the chimeric virus provided by the invention has stronger replication ability and better immunogenicity.

Isolated, recombinantly-generated, attenuated measles viruses and respiratory syncytial subgroup B viruses having defined attenuating mutations are described. Vaccines are formulated comprising such viruses and a physiologically acceptable carrier. The vaccines are used for immunizing an individual to induce protection against measles virus or respiratory syncytial subgroup B virus.

The invention aims to provide a recombinant canine measles virus expressing luciferase NLuc. The structure of a recombinant CMV nucleic acid fragment for rescuing and expressing NLuc is 5'-N-P-NLuc-M-F-H-L-3', wherein N, P, M, F, H and L are structural genes of the virus; NLuc is ORF of luciferase, the upstream of the ORF contains an M gene initiation sequence and a Not I restriction enzyme cutting site, and the downstream contains a Pme I restriction enzyme cutting site and a P gene termination sequence. The rCMV-NLuc rescued by a reverse genetic technology has a high expression level on NLucin cells, and can emit macroscopic light blue fluorescence under the action of a luminescent substrate furimazine. The insertion of an exogenous gene does not significantly affect the growth kineticsof the virus, and the exogenous gene is relatively stable in the virus passage process.

This invention comprises: compositions comprising a derivative, plasmids, a reagent kit and methods of making these compositions a derivative, vaccine- and non-vaccine-compositions of above for causing death of cancer cells that form part of a tumour and virus infected Dengue, Measles and other diseased cells; the derivative comprising replicating as well as non-replicating derivatives of an attenuated negative stranded RNA virus belonging to family paramyxoviridae, including Measles Virus, comprising a single additional transcriptional unit carrying either only one or two or more non-viral genes, and the non-replicating derivatives being free from contaminating replicating Measles Virus (b) a Measles Virus packaging cell line for making above compositions, expressing the M, F and H proteins of MV stably. And (c) a reagent kit for producing the Measles Virus derivatives described above.

The invention constructs a human embryo retina transformed cell line SL-2 by transforming adenovirus gene fragments into human embryo retina cells. The cell line can be used for producing biological products such as adenovirus and the like, and has obvious advantages. The cell line can also be used for producing influenza virus, herpes simplex virus, measles virus, rotavirus and other viruses, polypeptides and other biological products.

The invention relates to the field of virus detection, in particular to a nucleic acid sequence for isothermal amplification detection of measles virus, a kit, a detection method and application. Thenucleic acid sequence comprises strand displacement primer sequences shown as SEQ ID No. 1 and SEQ ID No. 2, probes shown as SEQ ID No. 3 and SEQ ID No. 4, and a cross amplification primer sequence shown as SEQ ID No. 5. The kit and the detection method have the technical advantages of good specificity, high sensitivity, simple steps and high repeatability.

The invention relates to the technical field of virus gene engineering, in particular to an H1a genotype chimeric measles virus attenuated strain and a preparation method and application of the H1a genotype chimeric measles virus attenuated strain. The method comprises the following steps: by taking a measles virus attenuated strain as a skeleton, and replacing the fusion protein coding gene F and the hemagglutinin protein coding gene H in the measles virus attenuated strain with nucleotide sequences of the fusion protein coding gene F and the hemagglutinin protein coding gene H containing the H1a genotype measles virus; and according to the present invention, the existing measles vaccine attenuated strain (such as Schwarz) is adopted as the skeleton, the immunogenic proteins F and H are respectively replaced with the H1a genotype related proteins F and H, and the constructed H1a genotype chimeric measles virus attenuated strain has the excellent protection effect on the H1a genotype measles virus.

The invention relates to the field of vaccines, in particular to a vaccine composition for inducing an HIV-1 broad-spectrum neutralizing antibody. A combined vaccine form is adopted, HIV-1 membrane protein is adopted as immunogen for the first time, and vaccine presentation forms include but are not limited to recombinant plasmid vaccines, recombinant protein subunit vaccines, recombinant virus vector vaccines, virus-like particle vaccines, nanoparticle vaccines, attenuated live vaccines and inactivated virus vaccines; the virus vector vaccine for expressing the HIV-1 membrane protein is adopted in the second injection, so that the titer and broad spectrum of an induced anti-HIV-1 neutralizing antibody can be remarkably improved; wherein the virus vector comprises but is not limited to poxvirus, adenovirus, herpes simplex virus, measles virus, enterovirus and rhabdovirus. And the third needle and the fourth needle are protein nanoparticle vaccine and virus vector vaccine combinations or vice versa. Inoculation modes of the vaccine include but are not limited to muscle inoculation, intracutaneous inoculation, subcutaneous inoculation, nasal dripping, aerosol inhalation, genital tract, rectum, oral administration and the like. Animal experiment results prove that the vaccine combination is safe, can continuously generate a high-titer broad-spectrum neutralizing antibody, and canbe used for preventing and treating AIDS in the future.

A result of the detection of normal and abnormal absorption spectrum of MCF-7, HeLa cells, measles viruses and the like through an ultraviolet absorption spectrum energy measurement technique shows that characteristic absorption peak curves mean the interaction of DNA and proteins in cells. The shape of curves of a same substance with different concentrations is completely same. A cancer cell gene isomer is a long-lived excited nuclide, and has a blue shift of 5-8nm from the maximum absorption wavelength of proto-oncogene, so a nuclear binding energy-gene threshold value is the measurement of the nuclear stability degree. The maximum gene barrier height lambdamax is 255.84nm, and DNA fission (canceration) is carried out when the maximum gene barrier exceeds gene saddle point height. A true tumor early stage diagnosis technology is used for detecting the gene threshold value barrier height. The multiplication of cancer cells and the secretion of oncofetal antigens appear after the cell canceration, and the cancer cells and the oncofetal antigens are cancer later stage detection products. The ultraviolet absorption spectrum energy measurement technology can rapidly detect the gene threshold value DNA quantum and momentum (Schrodinger quantum mechanics), and is excellent as a tumor early diagnosis technology.

The invention relates to application of glucocorticoid in preparation of a medicine for treating and/or preventing respiratory virus infection. The respiratory viruses are selected from SARS-CoV virus, SARS-CoV-2 virus, Middle East respiratory syndrome coronavirus, influenza virus, measles virus, parotitis virus, respiratory syncytial virus, parainfluenza virus, human metapneumovirus, Hendea virus, Nipie virus, rubella virus, rhinovirus, adenovirus, reovirus, coxsackie virus and ECHO virus.

The invention discloses a recombinant measles virus and a preparation method and application thereof, and belongs to the field of reverse genetics application. According to the recombinant measles virus, a measles virus is used as a carrier, a target gene is inserted in a noncoding region ahead of a 5' end of an N gene, or a noncoding region between a P gene and an M gene, or a noncoding region between an H gene and an L gene, and the target gene is a green fluorescent protein gene. An open reading frame of the target gene is inserted into three different positions of an overall-length transcription carrier of the S191 measles virus, three recombinant plasmids for expressing target protein are obtained, cells are transfected, and through saving, a recombinant measles virus strain is obtained. The virus strain can efficiently express an exogenous gene, is high in saving efficiency and good in stability, can be used for improving preparation of measles vaccines and providing reference ofinsertion sites for recombinant measles virus vaccines carrying other virus antigen protein genes, for example, the expression of fluorescent protein facilitates observation of an infection process of virus vaccine strains, and the recombinant measles virus has wide application value in the respect of oncolytic treatment.

The invention discloses MNP marker sites of measles virus, a primer composition, a kit and application of the MNP marker sites, the primer composition and the kit, and the MNP marker sites are genome regions which are screened on a measles virus genome, are distinguished from other species and have a plurality of nucleotide polymorphisms in the species, and comprise marker sites of MNP-1 to MNP-5; the primers are as shown in SEQ ID NO. 1 to SEQ ID NO. 10. The MNP marker site can be used for specifically identifying the measles virus and finely distinguishing different subtypes; the primer does not interfere with each other, integrates multiple amplification and sequencing technologies, can perform sequence analysis on all marker sites of multiple samples at one time, has the advantages of high throughput, multiple targets, high sensitivity and no culture, can be applied to measles virus identification and genetic variation detection of large-scale samples, and has important significance in scientific research and monitoring of measles viruses.