Belonging to the technical field of
biomedicine, the invention relates to a sequence and structure of a polypeptide able to specifically bind with influenza
virus hemagglutinin protein, and application of the polypeptide in anti-influenza viruses. By expressing purified influenza
virus hemagglutinin protein and screening a
random peptide library with a
phage display technology, a polypeptide specifically bound with influenza
virus hemagglutinin and equipped with sequences numbered 1-18 can be obtained. As a hemagglutinin-
binding peptide can hinder the combination of hemagglutinin and a host
cell receptor, so the influenza virus can be inhibited from infecting the host
cell. Thus, the invention also conducts an anti-influenza virus activity study to the hemagglutinin-
binding peptide selected from the phase
peptide library, and finds that a polypeptide H17, with a sequence of NH2-SHGRITFAYFAN-COOH, can effectively inhibit the influenza virus from infecting the host
cell and is of small
toxicity. Therefore, the hemagglutinin-
binding peptide of the invention and the H17 polypeptide therein with an anti-influenza virus activity are expected to become novel treatment medicaments for treating diseases caused by influenza virus infection and reducing the hazards of diseases caused by influenza viruses.