RHVT (recombinant Herpesvirus of Turkey)-H9HA (H9 hemagglutinin) and construction method thereof

Abstract

The invention relates to an rHVT (recombinant Herpesvirus of Turkey)-H9HA (H9 hemagglutinin) for expressing H9 subtype AIV (avian influenza virus) HA protein and a construction method thereof. The collection number of a vaccine strain rHVT-H9HA is CGMCC No: 10907. A GFP (green fluorescent protein) expression cassette is separated from a carrier pEGFP-C1 (plasmid enhanced green fluorescent protein) and is inserted into an HVT genome, and a recombinant virus rHVT-GFP is obtained. Through homologous recombination, a GFP gene of the rHVT-GFP is replaced with an HA gene of H9N2 subtype AIV epidemic strain A / Chicken / Jiangsu / WJ57 / 2012, a recombinant virus without fluorescence is selected, and the rHVT-H9HA for stably expressing the H9 subtype AIV HA gene is obtained. The recombinant virus strain is low in cost, good in safety, long in immunity period and suitable for large-scale production of vaccine and can be used for making the vaccine.

Description

technical field [0001] The invention relates to the field of recombinant virus vaccines, more specifically, the invention relates to a turkey herpes virus strain rHVT-H9HA expressing the H9 subtype HA gene of avian influenza, which is used for the development of the vaccine. The invention also provides a method for developing the recombinant turkey herpes virus vaccine strain. Background technique [0002] Among the many pathogens that cause poultry diseases, avian influenza virus (Avian influenza virus, AIV) and Marek's disease virus (Marek's disease virus, MDV) occupy an important position. Prevention and control of outbreaks of avian influenza and Marek's disease in poultry is of great significance to animal health, public health and the world economy. [0003] Avian influenza (Avian influenza, AI) is a viral infectious disease that seriously endangers the development of the global poultry industry. AIV belongs to the genus of influenza virus A in the Orthomyxoviridae f...

AI Technical Summary

Problems solved by technology

Due to the strong cell-binding properties of HVT, this brings great difficulties to the screening of HVT recombinant viruses

At present, the most commonly used method is the marker gene insertion method to screen recombinant viruses. However, the presence of marker genes has brought many disadvantages, such as immunizing animals with recombinant viruses carrying the LacZ gene of Escherichia coli. The expression of LacZ gene may interfere with the immune effect of recombinant viruses. ; In addition, immunizing animals with recombinant viruses carrying antibiotic genes will also make animals develop drug resistance

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