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Recombined newcastle disease virus LaSota attenuated vaccine strain for expressing avian influenza virus H9 subtype HA protein

A technology of avian influenza virus and attenuated vaccine, applied in antiviral agents, virus antigen components, medical preparations containing active ingredients, etc., can solve the problems of inconvenient use and high manufacturing cost

Active Publication Date: 2009-03-04
HARBIN VETERINARY RES INST CHINESE ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The existing inactivated avian influenza vaccine has the advantages of safety and good immune protection effect, but there are still disadvantages of high manufacturing cost and relatively inconvenient use [1，2，3]

Method used

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  • Recombined newcastle disease virus LaSota attenuated vaccine strain for expressing avian influenza virus H9 subtype HA protein
  • Recombined newcastle disease virus LaSota attenuated vaccine strain for expressing avian influenza virus H9 subtype HA protein
  • Recombined newcastle disease virus LaSota attenuated vaccine strain for expressing avian influenza virus H9 subtype HA protein

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Example 1 Construction and biological activity of recombinant Newcastle disease LaSota attenuated vaccine expressing avian influenza virus H9 subtype hemagglutinin (HA) protein

[0036] 1 Materials and methods

[0037] 1.1 Cells and viruses

[0038] BHK-21 cells (ATCC CCL-10), the medium is DMEM containing 10% fetal bovine serum; H9 subtype avian influenza strain A / Chicken / Shandong / 07 / 2 / H9N2 (SD / 07) was purchased from China The National Avian Influenza Reference Laboratory of Harbin Veterinary Research Institute of the Academy of Agricultural Sciences; Newcastle disease virus Beijing strain (F48E9) used in the challenge test was commercially purchased from the China Veterinary Drug Administration; the recombinant poxvirus vTF7-3 (ATCC, VR- 2153) was purchased from ATCC; Newcastle disease hemagglutination inhibition test (HI) diagnostic antigen was produced and provided by Harbin Veken Biotechnology Development Company; H9 subtype avian influenza virus hyperimmune serum...

Embodiment 2

[0064] Example 2 Pathogenicity Test of rL-H9HA Recombinant Virus and Immunization Test to Chicks

[0065] 1. Materials and methods

[0066] Materials and instruments are the same as in Example 1.

[0067] 1.1 Pathogenicity test of recombinant virus

[0068] The pathogenicity tests of the recombinant virus rL-H9HA, such as mean embryonic lethal time (MDT), chick intracerebral pathogenicity index (ICPI) and chicken intravenous pathogenicity index (IVPI), were carried out according to the O.I.E. standard [1，2] .

[0069] 1.2 Immunization test of recombinant virus on chicks

[0070]In order to evaluate the immunoprotective effect of the recombinant virus on SPF chicks, chicken embryos were amplified with allantoxin to 2x10 6 EID 50 Doses were administered via nasal drops and eye drops to artificially immunize 12 7-day-old white Leghorn SPF chicks (commercially purchased from the Harbin Veterinary Research Institute of the Chinese Academy of Agricultural Sciences), and 8 non-i...

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Abstract

The invention relates to a recombinant newcastle pestilence LaSota HCLV expressing the bird flu virus H9 subtype hemagglutinin (HA) protein, in particular the recombinant newcastle pestilence LaSota is rL-H9HA. The invention further discloses a method for preparing the recombinant newcastle pestilence LaSota HCLV and the application of the HCLV in preparing a bivalent vaccine for preventing bird flu and newcastle pestilence.

Description

technical field [0001] The present invention relates to the field of recombinant virus vaccines, more specifically, the present invention relates to a recombinant Newcastle disease LaSota attenuated vaccine expressing a gene encoding avian influenza virus H9 subtype hemagglutinin (HA) protein, more specifically, recombinant Newcastle disease LaSota The attenuated vaccine is rL-H9HA. The invention also discloses a method for preparing the recombinant Newcastle disease LaSota attenuated vaccine and the application of the recombinant Newcastle disease LaSota attenuated vaccine in preparing a bivalent vaccine for preventing bird flu and Newcastle disease. Background technique [0002] The Class A infectious diseases stipulated by the World Organization for Animal Health (OIE) include two severe poultry infectious diseases, namely highly pathogenic avian influenza and Newcastle disease. In addition to H5 or H7 subtype AIV, highly pathogenic avian influenza also has H9 subtype AI...

Claims

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Application Information

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IPC IPC(8): A61K39/295A61K39/17A61P31/14A61P31/16C12N15/45
Inventor 步志高陈化兰葛金英
Owner HARBIN VETERINARY RES INST CHINESE ACADEMY OF AGRI SCI
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