The invention discloses an ELISA (enzyme-linked immune sorbent assay) analytical method for directly detecting furaltadone metabolite AMOZ (3-amino-5-morpholinomethyl-2-oxazolidinone) content in food, which is characterized in that three different AMOZ hapten derivatives are synthesized and crosslinked with carrier protein to synthesize three immunogens and envelope antigens so as to prepare a monoclonal antibody by the hybridoma monoclonal antibody preparation technique. The AMOZ in a sample can be directly detected without derivatization steps by the setup ELISA, and the prepared monoclonal antibody is not in cross reaction with other nitrofurans and metabolite thereof and eight antibiotics, so that the specificity of the antibody is high. AMOZ of different amounts is added into four meat samples including fish, shrimp, chicken and pork liver, the ELISA is used for direct determination, the standard recovery rate ranges from 72.6% to 102.7%, the relative standard deviation is 6.1-17.7%, seven standard samples are detected by HPLC (high performance liquid chromatography) and ELISA simultaneously, determination results are compared, the regression curve is Y=0.9742X-7.6001, and the linearly dependent coefficient is 0.9889, so that the HPLC and the ELISA have high relativity.