345 results about "Monoclonal antibody preparation" patented technology

A stabilized human monoclonal antibody preparation containing 1 to 20 mg of D-mannitol per 1 mg of a human monoclonal antibody. This preparation is excellent in stability in a solution state, a freeze drying state and a freezing state, particularly stability against aggregation and precipitation of the human monoclonal antibody at the time of redissolution after freeze drying.

This invention relates to a monoclonal antibody capable of combining with H5 subtype avian influenza virus HA protein specifically, the hybridoma cell line secreting said antibody and a preparing method. The invention also relates to a serial test kit for testing H5 subtype avian influenza virus by the antibody and a bit of said antibody in the test sample of the virus and its usage in treatment.

The invention relates to an anti-human transferrin receptor human antibody and the application, which pertains to the field of molecular biology. The anti-human transferrin receptor human antibody has a single-chain antibody (scFv) amino acid sequence which is shown in a sequence table SEQ ID No.1. The anti-human transferrin receptor human antibody and the application have the advantages that: the human antibody solves the problem of immunogenicity of the target antibody, thus allowing the human antibody to carry out the long-term repeated drug administrations in the human body; the method for screening a genetic engineering antibody in a large-capacity antibody library which is adopted by the invention is easier than the monoclonal antibody preparation, the large-scale production is easy, the molecule of the antibody is small, and the antibody can enter tissues deeply, thus having obvious advantages. A pET-22b (plus) vector which is adopted by the invention contains a stronger T7 promoter, which can ensure the higher expression level of the target antibody. The induced expression conditions after the optimization can ensure that the antibody is expressed in a soluble form and the activity of protein is higher. The 3' end of the pET-22b (plus) vector is provided with six His(histidine) labels, which can easily use the Ni-NTA agarose to carry out affinity chromatography and purification.

The invention provides a genetic engineering cell strain which is constructed on the basis of CRISPR-Cas9 systems and is capable of secreting mouse interleukin-6. Target sequences of sgRNA for specifically targeting mouse ROSA26 genes are shown as SEQ ID NO.1, and site-specific cleavage vectors capable of simultaneously expressing the sgRNA and cas9 proteins are further constructed; donor vectorswith mouse interleukin-6 and ROSA gene homogenous sequences are further constructed, site-specific integration is carried out on the mouse interleukin-6 at ROSA26 sites of mouse cells SP2 / 0, selectionis carried out by the aid of selection markers to obtain homozygous cell strains, and the genetic engineering cell strain with a mouse interleukin-6 high-expression function can be obtained. The genetic engineering cell strain has the advantage that the genetic engineering cell strain can be used for producing hybridoma cells with high-proportion secretion capacity and has a broad application prospect in the field of monoclonal antibody preparation.

The present invention discloses a kind of monoclonal antibody for detecting type O foot and mouth disease virus and its preparation process and use.

The invention discloses an anti-influenza A virus nucleoprotein monoclonal antibody with high affinity and high specificity, its preparation and application. The anti-influenza A virus nucleoprotein monoclonal antibody is secreted by a hybridoma cell strain H1N1-2F10, and the preservation number of the cell strain is CCTCC C201119. The invention also provides a kit prepared with monoclonal antibody for rapid detection of influenza A. The kit has the characteristics of simple operation, strong specificity and high sensitivity, etc.

The invention relates to preparation of troponin I specific locus antibody and a detection kit thereof. A fixed point hydrolysis method is adopted to obtain the required three peptide sections of human cardiac troponin I (cTnI); the three peptide section are orderly subjected to immunogenicity modification and animal immunization so as to obtain high titer immune serum; the three peptide sections extracted in the hydrolysis are taken as the ligand and the polyacrylamide is taken as genin to prepare an affinity column; and finally the obtained high titer immune serum is subjected to immunoaffinity chromatography so as to obtain polyclonal antibodies having specific recognition on the three peptide sections. The antibodies have the advantages that: the affinity of the antibodies is higher than that of a corresponding monoclonal antibody, the specificity is equal to that of a corresponding monoclonal antibody, moreover, the preparation cost is lower, and the preparation process is simpler, compared to that of a monoclonal antibody. The obtained three polyclonal antibodies can be made into an immunity latex kit for a semi-automatic or automatic generation analysis instrument for detecting the content of cardiac troponin I (cTnI) in serum.

The present invention discloses a cadmium chelate monoclonal antibody and a preparation method as well as application of the monoclonal antibody. The monoclonal antibody is of specificity to cadmium -1 - (4 - isothiocyanate-benzyl) - EDTA - bovine serum albumin and the cadmium metal chelate. The preparation method of the cadmium chelate monoclonal antibody is as following: first of all, hapten is prepared and cadmium chelate complete antigen is formed; spleen cells of mouse which are Cd-ITCBE-KLH-immune are combined with SP2 / 0, and the positive clones are screened through a two-step method; after a plurality of times of subcloning, monoclonal antibody that can stably excrete anti-cadmium chelate is obtained. The cadmium chelate monoclonal antibody is used to measure the concentration of cadmium ion through the linear equation: Y = 0.0081X +0.1115, R 2 = 0.9922. The measurement is fast and the cost is low; the measurement result is accurate and is of high sensitivity and strong selectivity; the equipment is simple and can be easily carried for on-spot testing.

The invention relates to a monoclonal antibody and an antibody composition for neutralizing canine distemper virus (CDV), belonging to the technical field of biology. The monoclonal antibody 2G3 disclosed by the invention has high activity for neutralizing CDV and is used for identifying epitopes differently acting on CDV with a monoclonal antibody for neutralizing CDV prepared from another screened hybridoma cell 1D7 strain. Two monoclonal antibodies are prepared into the antibody composition, the neutralizing capability on CDV of which is obviously prior to the single monoclonal antibodies 2G3 and 1D7 in the composition; after being combined, the two monoclonal antibodies can generate antiviral synergistic enhancement action; and both the range and the capability for neutralizing CDV are increased. The monoclonal antibody disclosed by the invention is used for preparing the antibody composition for treating clinical canine distemper paroxysm animal; the virus neutralizing capability is stronger; failure of the monoclonal antibody caused by CDV heteromorphosis can be avoided; and the clinical application range is wider.

The present invention provides a clenbuterol monoclonal antibody and applications thereof. The present invention discloses a clenbuterol monoclonal antibody preparation method, wherein clenbuterol hapten is synthesized, the synthesized clenbuterol hapten and carrier protein are coupled to obtain clenbuterol antigen, and the clenbuterol antigen is adopted to immunize animals to obtain a high specificity monoclonal antibody. The present invention further provides a method for application of the clenbuterol monoclonal antibody in a clenbuterol enzyme-linked immunosorbent assay kit to detect clenbuterol. The present invention further provides a method for application of the clenbuterol monoclonal antibody in a clenbuterol colloidal gold test paper card to detect clenbuterol. The prepared clenbuterol monoclonal antibody has characteristics of high specificity and low cost, wherein the clenbuterol drug detection clenbuterol enzyme-linked immunosorbent assay kit prepared by using the clenbuterol monoclonal antibody and the clenbuterol drug detection clenbuterol colloidal gold test paper card prepared by using the clenbuterol monoclonal antibody have characteristics of convenient operation, high specificity, high sensitivity, high accuracy, high precision, fast detection and the like.

The present invention discloses a reagent box for quickly detect chicken and birds flu virus antibody. This reagent box includes body, 96 aperture enzyme mark bar in the box, test paper and sample diluted liquid. The test paper consist of sample cushion, absorb cushion, besmear IgG Fc golden cushion, bundled with NP decect string and IgG quality string pyroxylin film, in turn absorb cushion, pyroxylin film, golden cushion, sample cushion plaster unsopped water supported slice. This invention relates to a kind of special monoclonal antibody cross bred tumour cell BDRPDP and special identify IgGFc monoclonal antibody preparation. The reagent box which detect chicken and birds flu virus has strong diathesis, higher sensitivity, simply manipulation and diagnose quickly distinct characteristic.

The invention provides a hybridoma prepared from canine parvovirus antigens. An anti-canine-parvovirus monoclonal antibody secreted from the hybridoma has quite high relative affinity constant and good neutralizing activity, and a vaccine composition prepared from the monoclonal antibody can effectively prevent and treat canine parvovirus infections. A canine parvovirus detection kit prepared with two kinds of monoclonal antibodies has the advantages of quickness, convenience, accuracy, good sensitivity, specificity and repeatability and has higher sensitivity than colloidal gold-labeled commercial test strips.

The invention discloses a monoclonal antibody preparing method of high-flux, which comprises the following steps: (a) proceeding immune inoculation for non-human mammal animal through multiple immunogen; (b) compounding splenocyte of immune inoculation animal and bone marrow oncocyte to fuse into cross oncocyte; (c) collecting the cross oncocyte; culturing; preparing cross oncocyte suspension; (d) screening cross oncocyte through biological chip to produce cross oncocyte system of immunogen monoclonal antibody.

The invention discloses a mouse monoclonal antibody against a human Siglec-15 protein. The specificity is high, the performance is stable and the titer is high. The invention further relates to application of the monoclonal antibody to preparation of immunohistochemical detection tools for the Siglec-15 protein. According to the mouse monoclonal antibody against the human Siglec-15 protein, an immunohistochemistry method is used for detecting expression of Siglec-15 on the tumor cell surface for selection, the curative effect and the prognosis of therapeutic schedules of malignant tumors. Theinvention further relates to the application of the monoclonal antibody to preparation of antibody drugs for immunotherapy of the malignant tumors. The important application value is achieved. The adopted antibody drugs can be full-length or partial sections (Fab, F(ab)'2 or ScFv) of an Siglec-15 monoclonal antibody or combined use of the Siglec-15 monoclonal antibody and a PD-L1 monoclonal antibody. The malignant tumors mainly include colorectal cancer, endometrial cancer, thyroid cancer, bladder cancer, kidney cancer, lung cancer, liver cancer and the like.

The invention discloses monoclonal antibody of anti-dengue virus E protein, which is generated through the secretion of the mice hybridoma cells 2B10 with the collection number of CGMCC No.2407; the monoclonal antibody provided by the invention has the advantages that the comprehensive effectiveness is high, the monoclonal antibody can be specifically combined with the dengue virus and can be applied to the preparation of the novel and effective medicine which can prevent and cure dengue virus infection and can also be manufactured into reagent kits for testing dengue virus.

A method for simultaneously detecting zearalenone and fumonisins in the technical field of biological detection engineering, comprising the following steps: 1. Prepare conjugates ZEN-BSA and ZEN-OVA; FB1-KLH and FB1-OVA; 2. Prepare anti-ZEN monoclonal antibody and anti-FB1 monoclonal antibody respectively; 3. Prepare colloidal gold, respectively label anti-ZEN monoclonal antibody and anti-FB1 monoclonal antibody; spray the labeled monoclonal antibody onto the gold label pad ; 4. Spotting on the nitrocellulose membrane; 5. Assemble into a test strip; 6. Drop the methanol extraction supernatant of the sample to be tested into the sample groove of the test strip to obtain the points on the nitrocellulose membrane. The color development result of the sample band, the identification, and the result are obtained. The detection object of the method of the present invention is highly targeted and has high accuracy; the detection speed is fast and the required time is short, the loss of manpower and material resources caused by using two detection methods to detect two toxins is reduced, and it is convenient for promotion and application at the grassroots level.

The invention discloses an Lp(a) (Lipoprotein(a)) resisting monoclonal antibody and a latex-enhanced immunonephelometric detection kit for Lp(a). The invention provides a hybridoma cell strain which can stably secrete the Lp(a) monoclonal antibody and has the collection number of CGMCC No. 8509. The hybridoma cell strain provided by the invention can stably produce the Lp(a) monoclonal antibody, and personal pairing, specific Lp(a) combination and no combination with Kringle IV-2 can be realized. Shown by a test that serum samples of patients are detected by an Lp(a) kit prepared from the Lp(a) resisting monoclonal antibody, values detected by the latex-enhanced immunonephelometric detection kit prepared from the Lp(a) resisting monoclonal antibody have high conformity with detected values of commercially available kits, and the accuracy is reliable, so that the latex-enhanced immunonephelometric detection kit can be applied to Lp(a) detection.

The invention relates to the technical field of preparation of monoclonal antibodies, and in particular relates to a preparation method of a monoclonal antibody and a kit thereof. The preparation method comprises the following steps: performing immunization on an animal by adopting an antigen, and taking immune spleen cells; fusing the immune spleen cells with myeloma cells to obtain fusion cells; detecting the fusion cells by adopting an indirect ELISA (enzyme-linked immunosorbent assay) method to obtain positive hybridoma cells; detecting the positive hybridoma cells by adopting a competitive inhibition ELISA method to obtain hybridoma cells with competitive reactions; sub-cloning the hybridoma cells with competitive reactions to obtain monoclonal antibody hybridoma cells; and excreting the monoclonal antibody hybridoma cells to obtain the monoclonal antibody. According to the preparation method provided by the invention, the monoclonal antibody is screened by adopting a method combining the indirect ELISA method and the competitive inhibition ELISA method, the operation is simple and convenient, the purposiveness is strong, the workload is small, and a high-quality hybridoma cell strain can be locked in a short time.

The invention relates to a humanized monoclonal antibody targeting Claudin18.2 as well as a preparation method and application thereof. According to the present invention, the Claudin18.2 humanized monoclonal antibody obtained through the cell fusion and the hybridoma technology can be highly specifically combined with CHO-Claudin18.2 cells, but is almost not combined with CHO-Claudin18.1 cells, the preparation method of the Claudin18.2 humanized monoclonal antibody of the present invention has simple steps, and the obtained Claudin18.2 humanized monoclonal antibody has good ADCC and CDC activity on the expression of Claudin18.2 positive tumor cells. The humanized monoclonal antibody of Claudin18.2 disclosed by the invention is high in specificity and small in side effect, and has a very good prospect in application to treatment and / or prevention or diagnosis of Claudin18.2-related diseases such as tumors.

The invention discloses an ELISA (enzyme-linked immune sorbent assay) analytical method for directly detecting furaltadone metabolite AMOZ (3-amino-5-morpholinomethyl-2-oxazolidinone) content in food, which is characterized in that three different AMOZ hapten derivatives are synthesized and crosslinked with carrier protein to synthesize three immunogens and envelope antigens so as to prepare a monoclonal antibody by the hybridoma monoclonal antibody preparation technique. The AMOZ in a sample can be directly detected without derivatization steps by the setup ELISA, and the prepared monoclonal antibody is not in cross reaction with other nitrofurans and metabolite thereof and eight antibiotics, so that the specificity of the antibody is high. AMOZ of different amounts is added into four meat samples including fish, shrimp, chicken and pork liver, the ELISA is used for direct determination, the standard recovery rate ranges from 72.6% to 102.7%, the relative standard deviation is 6.1-17.7%, seven standard samples are detected by HPLC (high performance liquid chromatography) and ELISA simultaneously, determination results are compared, the regression curve is Y=0.9742X-7.6001, and the linearly dependent coefficient is 0.9889, so that the HPLC and the ELISA have high relativity.

The invention relates to a monoclonal antibody of solid particles of specific binding coxsackievirus A16 (CA16), and a conservative variant or an active fragment of the monoclonal antibody. The invention further relates to a method for detecting a CA16 solid particle antigen by using the monoclonal antibody and use of the monoclonal antibody for preparing drugs for preventing or detecting or treating CA16.

The invention discloses a method for detecting insecticidal crystal proteins Bt Cry1Ab / Ac and a special enzyme linked immunosorbent assay kit thereof. An anti-Bt Cry1Ab / Ac monoclonal antibody used by the method and the kit is secreted by a hybridoma cell strain Bt2F9, and the application number of the hybridoma cell strain in CGMCC (China General Microbiological Culture Collection Center) is CGMCC No.5162. The monoclonal antibody is obtained by simultaneously using a Bt Cry1Ac protein solution and an extracting solution of Bt Cry1Ac transgenic cotton plant leaves as a coating antigen and screening hybridoma cells through an indirect non-competitive ELISA (Enzyme-Linked Immuno Sorbent Assay) method, the monoclonal antibody has high affinity for Bt Cry1Ab / Ac protein expressed in plants, and the affinity constant is 1.03*10<9>M<-1>. The double-antibody sandwich enzyme linked immunosorbent assay kit prepared by the monoclonal antibody is used for qualitatively or quantitatively detecting proteins Bt Cry1Ab / Ac in transgenic plants such as cotton and corn, the linear detection range is 0.78-50ng / mL, and the kit has the advantages of fastness and sensitiveness.

The invention relates to a Siglec-15 monoclonal antibody and applications thereof. Through a cell fusion and hybridoma technology, the obtained human Siglec-15 monoclonal antibody can highly and specifically bind to the cells expressing human Siglec-15 antigen; the monoclonal antibody targeting human Siglec-15 is simple in preparation method; and the obtained anti-human Siglec-15 monoclonal antibody can well detect Siglec-15 protein expressed on tumor cells. The monoclonal antibody targeting the Siglec-15 is high in antibody specificity and has good application prospects on treating and / or preventing or diagnosing diseases, such as tumor, related to the Siglec-15.

The invention relates to an imidacloprid pesticide against monoclonal antibody and a preparation method thereof, and belongs to the technical field of biology. The preparation method comprises the following steps of: immunizing a BALB / c mouse by using a coupling substance of immune hapten 1-[6-(2-carboxyethylsulfenyl-3-pyridine) methyl]-N-nitro-2-imidazoline imine and bovine serum albumin, preparing hybrid tumor cells from spleen cells and myeloma cells Sp2 / 0 of the immunized mouse by the hybrid tumor technology, and obtaining hybrid tumor strains 2F11 / A9 capable of stably secreting the imidacloprid pesticide against monoclonal antibody. By effectiveness verification, the antibody can be used for sensitive and quick detection of imidacloprid residues in agricultural production environments and agricultural products. The preparation technique for the imidacloprid pesticide against monoclonal antibody is simple and feasible, does not need special instruments and equipment in the whole preparation process of the antibody, and is easy for scale production in factories.

The invention relates to a detection kit for saccharifying serum albumin by using indirect immunifaction. The kit comprises a specificity monoclonal antibody which is prepared for a specific glycosylated area of the serum albumin and can be used for identifying the specific non-glycosylated glycosylated area with high degree of specificity. The kit also comprises a monoclonal antibody for specifically identifying a counterpoint area of the specific glycosylated area, and an immunonephelometry reagent is prepared by the specific monoclonal antibody and the monoclonal antibody. The concentration of albumin which does not take part in saccharifying reaction in the specific area is measured by identifying the specificity monoclonal antibody in the specific non-glycosylated glycosylated area, the concentration of the total albumin is measured by using the method in the prior art, and the concentration of the saccharified albumin can be obtained by subtracting the concentration of the total albumin by the concentration of the albumin which does not take part in the saccharifying reaction. The monoclonal antibody disclosed by the invention is easy to prepare and relatively low in cost, is not limited by the foreign patent, and is convenient to widely apply and popularize.

The invention relates to a hybridoma cell strain secreting a high-neutralization activity infectious bursal disease virus (IBDV) monoclonal antibody, and belongs to the field of biotechnology. The hybridoma cell strain C128 is screened from an established bank of hybridoma cells secreting the IBDV monoclonal antibody, and a mice ascites monoclonal antibody prepared by the hybridoma cell strain has IBDV neutralization titer of 1010. The high-neutralization activity IBDV monoclonal antibody secreted by the hybridoma cell strain C128 has high neutralization titer and a wide IBDV strain resistance range, can be used for clinical treatment on IBD-infected animals, and has obvious curative effects, good safety and no adverse side reaction. A monoclonal antibody preparation prepared by the hybridoma cell strain C128 keeps neutralization titer after being preserved for 2 years and has good stability.

The invention discloses a glycocholic acid specific semiantigen, a preparation method for a glycocholic acid specific monoclonal antibody based on the semiantigen, and an immunity chromatography detection method for detecting glycocholic acid. The above specific monoclonal antibody is obtained from a glycocholic-acid-specific-semiantigen-coupled protein immune animal. The immunogen prepared through coupling of the glycocholic acid specific semiantigen and protein keeps a specific active site of glycocholic acid, and the cross reaction rates of the obtained monoclonal antibody with other 8 kinds of bile acids are all smaller than 10%. A time-resolved immunochromatographic test strip prepared by using the above glycocholic acid specific monoclonal antibody is capable of rapidly determining the content of glycocholic acid in a sample, and specificity and accuracy of current glycocholic acid clinic diagnosis are improved.

The invention relates to an anti-human transferrin receptor human antibody and the application, which pertains to the field of molecular biology. The anti-human transferrin receptor human antibody has a single-chain antibody (scFv) amino acid sequence which is shown in a sequence table SEQ ID No.1. The anti-human transferrin receptor human antibody and the application have the advantages that: the human antibody solves the problem of immunogenicity of the target antibody, thus allowing the human antibody to carry out the long-term repeated drug administrations in the human body; the method for screening a genetic engineering antibody in a large-capacity antibody library which is adopted by the invention is easier than the monoclonal antibody preparation, the large-scale production is easy, the molecule of the antibody is small, and the antibody can enter tissues deeply, thus having obvious advantages. A pET-22b (plus) vector which is adopted by the invention contains a stronger T7 promoter, which can ensure the higher expression level of the target antibody. The induced expression conditions after the optimization can ensure that the antibody is expressed in a soluble form and theactivity of protein is higher. The 3' end of the pET-22b (plus) vector is provided with six His(histidine) labels, which can easily use the Ni-NTA agarose to carry out affinity chromatography and purification.

The invention provides a flexible high-flux cell electric fusion microelectrode array chip device, which is composed of an array chip and a fusion pool. The array chip takes a flexible transparent polyimide film as a substrate, the lower surface forms a cross comb-shaped array multimicroelectrode through etching, the electrode group is composed of two comb-shaped microelectrode array electrodes which are mutually crossed but not contacted or connected in electrical structure, and a microchannel between both internal electrodes of the electrode group serves as a service passage; the array chipis inversely buckled on the fusion pool, and the cross comb-shaped array multimicroelectrode on the array chip corresponds to the cell electric fusion pool and falls in the cell electric fusion pool.The invention has the advantages that the use is convenient, the processing method is simple, the cost is extremely inexpensive, and the chip device can not cause damages to operators or cells, thereby being suitable for being widely applied in the fields of genetics, animal and plant distant hybridization breeding, developmental biology, medicine screen, mono-clonal antibody preparing and mammalian clone.

The invention discloses an enzyme-linked immuno sorbent assay (ELISA) method for detecting the melamine content in a food. The method is characterized in that three different melamine hapten derivatives are synthesized and are linked with a carrier protein to synthesize three immunogens and three envelope antigens; and a monoclonal antibody is prepared by using a hybridoma monoclonal antibody preparation technology. Different amounts of melamine are added into four samples, namely pure milk, milk powder, chicken feeds and pig feeds, and are directly measured by ELISA; the labeling recycling rate is 72.6 to 133.3 percent; the relative standard deflection is 0.8 to 18.9 percent; when the samples are labeled, measured results are detected and compared by using high performance liquid chromatography (HPLC) and ELISA; a regression curve is Y which is equal to 1.5589x-1.4004; a linear relevant coefficient is 0.9902; and a result shows that the two methods are relatively high in relevance.