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Detection kit for saccharifying serum albumin by using indirect immunifaction and measuring method

A technology of serum albumin and detection kit, which is applied in the field of indirect immunoassay glycosylated serum albumin detection kit, can solve the problems of difficulty in obtaining antibodies, limited development and application of reagents, difficulty in antibody preparation, etc., and achieves wide application and The effect of promotion, low cost and easy preparation

Active Publication Date: 2013-02-06
SICHUAN XINCHENG BIOLOGICAL CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

These monoclonal antibodies that directly recognize the glycosylated amino acid region are currently difficult to prepare antibodies, and it is difficult to obtain antibodies, and the development and application of reagents are limited.

Method used

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  • Detection kit for saccharifying serum albumin by using indirect immunifaction and measuring method
  • Detection kit for saccharifying serum albumin by using indirect immunifaction and measuring method
  • Detection kit for saccharifying serum albumin by using indirect immunifaction and measuring method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0055] The indirect immunoassay glycosylated serum albumin detection kit in this example consists of reagent 1 (R1) and reagent 2 (R2), and its specific formula (formula 1) is as follows (weight percentage):

[0056] R1：

[0057] Phosphate buffer (PH6.0): 2%

[0058] NaCl: 1.2%

[0059] PEG6000: 3%

[0060] Tween-20: 2%

[0061] Interference Elimination Protein IEPS: 0.05%

[0062] Preservative ProClin300: 0.03%

[0063] Purified water: 91.75%

[0064] R2：

[0065] Phosphate buffer (PH6.0-8.0): 2%

[0066] PEG6000: 1%

[0067] Stabilizer: 5%

[0068] Preservative ProClin300: 0.03%

[0069] Anti-human serum albumin non-glycosylated Lys525 region monoclonal antibody (1.0mg / ml): 1%

[0070] Anti-human serum albumin monoclonal antibody (Lys525 opposite Val 120 region) (1.0mg / ml): 1%

[0071] Purified water: 90%.

Embodiment 2

[0073] The indirect immunoassay glycosylated serum albumin detection kit in this example consists of reagent 1 (R1) and reagent 2 (R2), and its specific formula (formula 2) is as follows (weight percentage):

[0074] R1：

[0075] Phosphate buffer (PH6.0): 2%

[0076] NaCl: 2.4%

[0077] PEG6000: 4%

[0078] Tween-20: 2%

[0079] Interference Elimination Protein IEPS: 0.05%

[0080] Preservative ProClin300: 0.03%

[0081] Purified water: 89.55%

[0082] R2：

[0083] Phosphate buffer (PH6.0-8.0): 2%

[0084] PEG6000: 2%

[0085] Stabilizer: 8%

[0086] Preservative ProClin300: 0.03%

[0087] Anti-human serum albumin non-glycosylated Lys525 region monoclonal antibody (0.5mg / ml): 0.5%

[0088] Anti-human serum albumin monoclonal antibody (Lys525 to Ala 320 region) (0.5mg / ml): 0.5% Purified water: 87%.

Embodiment 3

[0090] The indirect immunoassay glycosylated serum albumin detection kit in this example consists of reagent 1 (R1) and reagent 2 (R2), and its specific formula (formula 3) is as follows (weight percentage):

[0091] R1：

[0092] Phosphate buffer (PH6.0): 2%

[0093] NaCl: 3.5%

[0094] PEG6000: 5%

[0095] Tween-20: 2%

[0096] Interference Elimination Protein IEPS: 0.05%

[0097] Preservative ProClin300: 0.03%

[0098] Purified water: 87.45%

[0099] R2：

[0100] Phosphate buffer (PH6.0-8.0): 2%

[0101] PEG6000: 3%

[0102] Stabilizer: 10%

[0103] Preservative ProClin300: 0.03%

[0104] Anti-human serum albumin non-glycosylated Lys525 region monoclonal antibody (0.1mg / ml): 0.1%

[0105] Anti-human serum albumin monoclonal antibody (Lys525 to Thr 420 region) (0.1mg / ml): 0.1%

[0106] Purified water: 84.8%.

[0107] The formulations of Examples 1-3 can be used to measure the concentration of non-glycosylated albumin in the glycosylated region (B) by immunoturb...

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Abstract

The invention relates to a detection kit for saccharifying serum albumin by using indirect immunifaction. The kit comprises a specificity monoclonal antibody which is prepared for a specific glycosylated area of the serum albumin and can be used for identifying the specific non-glycosylated glycosylated area with high degree of specificity. The kit also comprises a monoclonal antibody for specifically identifying a counterpoint area of the specific glycosylated area, and an immunonephelometry reagent is prepared by the specific monoclonal antibody and the monoclonal antibody. The concentration of albumin which does not take part in saccharifying reaction in the specific area is measured by identifying the specificity monoclonal antibody in the specific non-glycosylated glycosylated area, the concentration of the total albumin is measured by using the method in the prior art, and the concentration of the saccharified albumin can be obtained by subtracting the concentration of the total albumin by the concentration of the albumin which does not take part in the saccharifying reaction. The monoclonal antibody disclosed by the invention is easy to prepare and relatively low in cost, is not limited by the foreign patent, and is convenient to widely apply and popularize.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a detection kit for glycosylated serum albumin by an indirect immune method. Background technique [0002] Diabetes (diabetes) is a sugar, protein, A series of metabolic disorder syndromes including fat, water and electrolytes, clinically characterized by hyperglycemia, polyuria, polydipsia, polyphagia, weight loss, etc. Blood sugar) Once it is not well controlled, it will cause complications, leading to failure and lesions of the kidneys, eyes, feet and other parts, and it cannot be cured. [0003] At present, the routine clinical examination is to detect the blood glucose concentration of the patient, but the blood glucose measurement can only represent the immediate blood glucose level, and cannot show the change of the blood glucose concentration over a period of time, so it cannot be used as an indicator for evaluating the degree of disease control. [0004] Glucose in the blo...

Claims

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Application Information

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IPC IPC(8): G01N33/68
Inventor 王钊赵艾钧谭韬
Owner SICHUAN XINCHENG BIOLOGICAL CO LTD
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