36 results about "Albumin concentrations" patented technology

The invention relates to a protein separation and purification process method in the biological product technology, in particular to a human serum albumin separation method, comprising protein separation and purification process. The method is characterized in that the method takes plasma as raw material; first of all, composition I, composition II and composition III are respectively precipitated; then the composition I, II and III are separated together; finally compositions IV-1 and IV-4 are precipitated respectively and separated together; the solid-liquid separation uses a pressure filtration technology, with the fluid intake pressure no more than 0.2Mpa. The method of the invention adopts the pressure filtration technology in the separation process, adjusts and controls the ethanol albumin concentration, temperature, pH value and other parameters in the albumin separation, and overcomes the disadvantages that the existing technology which produces human albumin through low-temperature ethanol separation has lower albumen composition yield and the unstable substances (such as lipoprotein) in the albumin composition are not completely removed. Compared with the low-temperature ethanol method, the method of the invention has high yield of albumin (not less than 2.9g / 100ml) and good product quality.

The invention relates to a detection kit for saccharifying serum albumin by using indirect immunifaction. The kit comprises a specificity monoclonal antibody which is prepared for a specific glycosylated area of the serum albumin and can be used for identifying the specific non-glycosylated glycosylated area with high degree of specificity. The kit also comprises a monoclonal antibody for specifically identifying a counterpoint area of the specific glycosylated area, and an immunonephelometry reagent is prepared by the specific monoclonal antibody and the monoclonal antibody. The concentration of albumin which does not take part in saccharifying reaction in the specific area is measured by identifying the specificity monoclonal antibody in the specific non-glycosylated glycosylated area, the concentration of the total albumin is measured by using the method in the prior art, and the concentration of the saccharified albumin can be obtained by subtracting the concentration of the total albumin by the concentration of the albumin which does not take part in the saccharifying reaction. The monoclonal antibody disclosed by the invention is easy to prepare and relatively low in cost, is not limited by the foreign patent, and is convenient to widely apply and popularize.

The invention belongs to the technical field of medical examination and particularly relates to a kit for measuring a concentration ratio of glycolated serum albumin and albumin. The kit comprises a reagent R1, a reagent R2, a reagent R3 and a reagent R4, wherein the reagent I contains a protein denaturant, hydroxyl-alkyl amine, trypsin, an anti-interference agent, a preservative and a buffer solution; the reagent II contains fructose amino acid oxidase, trehalose and a buffer solution; the reagent III contains amino acid oxidase, trehalose and a buffer solution; and the reagent IIII is a luminescent substrate 1,2-dioxetane boronic acid. The kit provided by the invention is similar with an existing enzymatic method detection kit in detection principle, but is lower in cost, high in interference resistance, accurate in detection, stable in property and convenient for extensive use.

The present invention is directed to tissue sealant compositions comprising: a multi-arm reactive polyethylene glycol polymer having at least 3 electrophilic groups; albumin; a buffer; water; and entrained gas as bubbles; wherein concentration of albumin in a liquid component of the sealant is within range of 50-200 mg / ml; and wherein concentration of multi-arm PEG in said liquid component of the sealant is within range of 25-100 mg / mL.