35 results about "Albumin concentrations" patented technology

The present invention is directed to tissue sealant compositions comprising: a multi-arm reactive polyethylene glycol polymer having at least 3 electrophilic groups; albumin; a buffer; water; and entrained gas as bubbles; wherein concentration of albumin in a liquid component of the sealant is within range of 50-200 mg/ml; and wherein concentration of multi-arm PEG in said liquid component of the sealant is within range of 25-100 mg/mL and wherein PEG-SG portion is composed of a blend of 2 PEG-SG different in arm number or arm lengths.

The invention relates to a detection kit for saccharifying serum albumin by using indirect immunifaction. The kit comprises a specificity monoclonal antibody which is prepared for a specific glycosylated area of the serum albumin and can be used for identifying the specific non-glycosylated glycosylated area with high degree of specificity. The kit also comprises a monoclonal antibody for specifically identifying a counterpoint area of the specific glycosylated area, and an immunonephelometry reagent is prepared by the specific monoclonal antibody and the monoclonal antibody. The concentration of albumin which does not take part in saccharifying reaction in the specific area is measured by identifying the specificity monoclonal antibody in the specific non-glycosylated glycosylated area, the concentration of the total albumin is measured by using the method in the prior art, and the concentration of the saccharified albumin can be obtained by subtracting the concentration of the total albumin by the concentration of the albumin which does not take part in the saccharifying reaction. The monoclonal antibody disclosed by the invention is easy to prepare and relatively low in cost, is not limited by the foreign patent, and is convenient to widely apply and popularize.

The invention relates to a protein separation and purification process method in the biological product technology, in particular to a human serum albumin separation method, comprising protein separation and purification process. The method is characterized in that the method takes plasma as raw material; first of all, composition I, composition II and composition III are respectively precipitated; then the composition I, II and III are separated together; finally compositions IV-1 and IV-4 are precipitated respectively and separated together; the solid-liquid separation uses a pressure filtration technology, with the fluid intake pressure no more than 0.2MPa. The method of the invention adopts the pressure filtration technology in the separation process, adjusts and controls the ethanol albumin concentration, temperature, pH value and other parameters in the albumin separation, and overcomes the disadvantages that the existing technology which produces human albumin through low-temperature ethanol separation has lower albumen composition yield and the unstable substances (such as lipoprotein) in the albumin composition are not completely removed. Compared with the low-temperature ethanol method, the method of the invention has high yield of albumin (not less than 2.9g/100ml) and good product quality.

The invention belongs to the technical field of medical examination and particularly relates to a kit for measuring a concentration ratio of glycolated serum albumin and albumin. The kit comprises a reagent R1, a reagent R2, a reagent R3 and a reagent R4, wherein the reagent I contains a protein denaturant, hydroxyl-alkyl amine, trypsin, an anti-interference agent, a preservative and a buffer solution; the reagent II contains fructose amino acid oxidase, trehalose and a buffer solution; the reagent III contains amino acid oxidase, trehalose and a buffer solution; and the reagent IIII is a luminescent substrate 1,2-dioxetane boronic acid. The kit provided by the invention is similar with an existing enzymatic method detection kit in detection principle, but is lower in cost, high in interference resistance, accurate in detection, stable in property and convenient for extensive use.

The invention discloses a micromolecule fluorescent probe and application thereof .The fluorescent probe is of the structure shown in the general formula I based on the TICT mechanism .Background fluorescence of the micromolecule fluorescent probe is weak in PBS buffer liquid, and the fluorescence intensity is obviously increased after the probe enters a hydrophobic cavity of human serum albumin HSA .The micromolecule fluorescent probe is good in selectivity and not disturbed by other common amino acid and protein molecules .In addition, response time is short after the probe interacts with HSA, and the probe can achieve specific binding of a FA1 locus of HSA .In the PBS buffer liquid, the fluorescence intensity and the HSA concentration show the good linear relation, and accurate detection can be achieved for human serum albumin HSA .In the real urine testing environment, the fluorescence intensity of the micromolecule fluorescent probe and albumin concentration in urine have the good linear relation, and therefore the micromolecule fluorescent probe has the good biological application prospect.

The invention relates to a quick nondestructive testing method of albumin products and particularly relates to an analysis method used for predicting albumin concentration through the characteristic spectrum segment of albumin and further verifying the identity of albumin products. The invention further relates to an analysis device executing the analysis method and an analysis system comprising the analysis device. The method, the device and the system provided by the invention combine a laser Raman spectrum with a partial least squares method to predict albumin contents in the albumin products, and compared with other quantitative techniques, the method, the device and the system have the advantages of simplicity in operation, rapid analysis, and the like.

The present invention relates to a method and system for using a hemofilter to treat IMRD, hepatic failure, exogenous intoxication and other conditions associated with toxins in a patient's blood. One treatment includes the use of a very large pore hemofilter to remove target complex molecules and/or target molecules from a patient's blood and to infuse a replacement fluid into the patient's blood to maintain a prescribed albumin concentration in the patient's blood.

The method includes steps: chemical reaction produces stable single oxygen source; reaction between single state oxygen and high selectivity, high sensitive chemiluminescence probe produces intermediate product in high energy, and sending out photons caused by deexcitation quickly; albumin in minute quantities combined with FCLA enhances sensitivity of chemiluminescence intensity; it is linear relation between magnitude of chemiluminescence enhanced sensitivity and concentration of albumin; using change of luminous intensity corresponding to change of concentration of albumin can detect albumin in trace amount. Equipment for implementing the method includes darkroom, sample cell, sample injector in minute quantities, integrating sphere, module for receiving chemiluminescence, module for treating electrical pulse, and computer etc. Features are: small sample needed, short time consumption, low cost, high sensitivity, and certain degree of interference immunity.

The invention discloses a traditional Chinese medicine feed additive for improving immune function of pigs as well as a preparation method and application of the additive. The additive is prepared from the following components in parts by weight: astragalus membranaceus, fructus alpiniae oxyphyllae, Chinese yam, endothelium corneum gigeriae galli, poria cocos, hawthorn, pinellia ternate, rhizoma atractylodis, ginger, fructus amomi and liquorice. The preparation method comprises the steps of drying, smashing, screening and uniformly mixing the raw materials by adopting a traditional Chinese medicine production device to prepare, adding a prepared traditional Chinese medicine preparation into basic ration of a small healthy Meishan pig of 60 days, uniformly blending to feed, and carrying out free choice feeding and free water drinking. Upon verification of tests, the method can obviously improve the concentration of total protein and albumin in porcine serum; can significantly improve the content of immune globulin IgG and the content of a complement C3 in the porcine serum; obviously improves the antibody level of post-inoculation swine fever; increases the pig spleen indexes. Moreover, the defects of various chemicals and antibiotics feed additives are overcome, toxic and side effects and drug residues do not exist, and the body immune function is improved while the production of green and high-quality animal products is guaranteed.

The invention discloses a method for detecting concentration of serum albumin by a fluorescent carbon dot probe from roasted chicken. The method comprises the following steps: step 1, mixing fluorescent carbon dot solutions with different concentrations extracted from the roasted chicken with a human serum albumin solution to prepare standard solutions with different concentrations, detecting fluorescence intensity of each standard solution to obtain fluorescence spectrograms of the standard solutions, taking the difference between fluorescence intensity when the concentration of human serum albumin is zero and fluorescence intensity of the standard solutions with other concentrations as dependent variables, and establishing a linear relationship with the concentration of human serum albumin; step 2, randomly preparing a human serum protein sample solution containing fluorescent carbon dots, detecting concentration of serum albumin in the sample, and detecting concentration of serum albumin in the sample solution through the linear relationship. According to the method, the concentration of serum protein in the solution is detected through the linear relationship, the method has simple and convenient detection process, high sensitivity and low detection limit, and online in-situ rapid and sensitive detection of hemoglobin concentration of an actual sample can be realized.

An object of the present invention is to provide a method for predicting prognosis for a cancer patient. The present invention provides a method for predicting prognosis for a cancer patient, wherein at least one index selected from the group consisting of an index of nutritional state, an index of sugar metabolism state, and an index of inflammatory state in the cancer patient is used as an index of prognosis. The index of nutritional state is preferably a concentration of albumin in the blood, the index of sugar metabolism state is preferably a concentration of glucose in the blood, and the index of inflammatory state is preferably a concentration of CRP in the blood.

The invention relates to the technical field of blood detection, in particular to a reagent and method for detecting whether the added sample amount is abnormal or not. According to the method, human serum albumin in a to-be-detected sample is detected based on a fluorescence reaction of albumin and albumin blue, a reasonable emission fluorescence acceptance range is set according to the sample adding amounts of different items, and if the acceptance range is exceeded, the sample adding amount in the testing process is judged to be abnormal; and prompting that the test result is invalid and needs to be retested or prompting that the albumin concentration needs to be rechecked. Experiments show that the method provided by the invention is accurate and reliable, and can effectively reduce the risk of misinformation of results during detection.

The present invention relates to a resuscitation fluid and method for a cryopreserved human PBMC. The resuscitation fluid includes the main components: recombinant human albumin with the concentrationof 5 g/ml, transferring with the concentration of 1 g/ml, glutamine with the concentration of 0.365 ug/ml, insulin with the concentration of 3.5 ug/ml, vitamin B with the concentration of 0.68 mg/ml,dextranum with the concentration of 0.3 g/ml, sodium chloride with the concentration of 6.9 mg/ml and calcium chloride with the concentration of 0.12 mg/ml. The resuscitation fluid contains no plasmas, the toxic effect of DMSO to cells may be effectively neutralized, cellular metabolites may be decomposed, the viability of the cells may be improved, the stability of cell membranes may be enhanced, the survival rate of the resuscitated cells may be increased, the bioactivity achieved before cryopreservation is recovered to the maximum extent, and thus, an immune cell may be effectively inducedto be activated to form a target cell.

The present invention relates to a determination method and system thereof. The method includes: providing or receiving a urine sample; distributing the urine sample into a first urine sample and a second urine sample; measuring a total protein concentration of the first urine sample; measuring a creatinine concentration and an albumin concentration of the second urine sample; and calculating a urine protein to creatinine ratio (UPCR) and a urine albumin to creatinine ratio (UACR), in which the UPCR is defined as a ratio of the total protein concentration to the creatinine concentration, and the UACR is defined as a ratio of the albumin concentration to the creatinine concentration.