Method for preparing monoclonal antibody resisting O-type foot and mouth disease virus and antibody and use

A foot-and-mouth disease virus and monoclonal antibody technology, applied in chemical instruments and methods, immunoglobulins, material testing products, etc., can solve the problems of undiagnosed virus, high speed and specificity, and achieve high specificity and antibody titer. High, sensitive effect

Inactive Publication Date: 2007-01-24
LANZHOU INST OF VETERINARY SCI CHINESE ACAD OF AGRI SCI
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Problems solved by technology

However, no effective, rapid and specific expertise fo

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[0018] The following is a specific preparation process introduction of the present invention:

[0019] (1) BHK-21 cells cultured in a spinner bottle were first used to proliferate O-type foot-and-mouth disease virus, and the virus liquid was collected. The virus liquid was frozen and thawed 3 times. 1% BEI was used to inactivate at 30°C for 28 hours and terminated with 2% sodium thiosulfate. After centrifuging at 8000rpm for 35min to remove cell debris, add PEG6000 at an amount of 80 grams per liter of liquid, add NaCl at an amount of 40 grams per liter of liquid, stir at 4°C for 12 hours, and overnight at 4°C; centrifuge at 8000rpm / min for 35min to precipitate the virus; Suspend the precipitate with TNE (0.1M Tris, 0.5M NaCl, 0.05M EDTA, pH7.6) to make the volume 1% of the original volume. Add 2 times the volume of trichlorethylene to degrease. Centrifuge at 12000rpm for 20min. Take the supernatant into a new centrifuge tube and centrifuge at 30,000rpm for 150min; resuspe...

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Abstract

The present invention discloses a kind of monoclonal antibody for detecting type O foot and mouth disease virus and its preparation process and use.

Description

technical field [0001] The invention relates to a method for preparing a monoclonal antibody for detecting O-type foot-and-mouth disease virus, the antibody, and the application of the antibody. Background technique [0002] Foot-and-mouth disease is a major animal disease, and all countries in the world attach great importance to the inspection and quarantine, prevention and control of the disease. At present, the main method of detecting and diagnosing FMD is to detect the FMD virus and its antibody level in the diseased or infected animals, such as virus isolation, ELISA and other methods. Direct isolation of virus is the most reliable diagnosis of foot-and-mouth disease, but a longer cycle will delay the best control time of this severe infectious disease; ELISA is simple and easy, but it needs to distinguish whether it is natural infection or antibodies produced by vaccine immunity. The diagnosis cannot be made in time. In order to facilitate the accurate and timely d...

Claims

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Application Information

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IPC IPC(8): C07K16/20C07K1/36G01N33/53G01N33/577
Inventor 常惠芸杜惠芬邵军军李克生吴海波林彤独军政丛国正谢庆阁
Owner LANZHOU INST OF VETERINARY SCI CHINESE ACAD OF AGRI SCI
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