Method for detecting furaltadone metabolite content in food

A technology for furaltadone and metabolites, which is applied in the field of food safety supervision or food analysis, and enzyme-linked immunosorbent adsorption analysis. It can solve the problems that AMOZ cannot be directly measured, and achieve the effects of low test cost, large test volume and high sensitivity.

Active Publication Date: 2012-08-01
SUZHOU UNIV
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  • Abstract
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  • Claims
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Problems solved by technology

However, it still cannot directly measure AMOZ, but needs to be derivatized with 2-nitrob

Method used

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  • Method for detecting furaltadone metabolite content in food
  • Method for detecting furaltadone metabolite content in food
  • Method for detecting furaltadone metabolite content in food

Examples

Experimental program
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Embodiment

[0077] 1. Preparation of AMOZ modification

[0078] AMOZ is a small molecular compound, which has no immunogenicity and cannot directly immunize animals to produce antibodies. The molecular structure of AMOZ must be chemically modified reasonably and effectively so that it has active groups so that it can be cross-linked with the carrier protein to obtain immune Original and coated antigens. The present invention has prepared three kinds of AMOZ modifiers:

[0079] (1) Synthesis of CPAMOZ I

[0080] Weigh 0.055-0.075mmol of 3-carboxybenzaldehyde (3-CBA) into the reaction bottle, dissolve it with as little anhydrous methanol as possible, then add 0.05-0.08mmol of AMOZ, after the dissolution is complete, react under nitrogen protection at 65°C for 5-12 After 1 hour, take it out, centrifuge the reaction solution, discard the supernatant clear night, and wash the precipitate three times with ice-free ethanol to obtain a white precipitate, which is vacuum-dried, with a yield of 7...

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Abstract

The invention discloses an ELISA (enzyme-linked immune sorbent assay) analytical method for directly detecting furaltadone metabolite AMOZ (3-amino-5-morpholinomethyl-2-oxazolidinone) content in food, which is characterized in that three different AMOZ hapten derivatives are synthesized and crosslinked with carrier protein to synthesize three immunogens and envelope antigens so as to prepare a monoclonal antibody by the hybridoma monoclonal antibody preparation technique. The AMOZ in a sample can be directly detected without derivatization steps by the setup ELISA, and the prepared monoclonal antibody is not in cross reaction with other nitrofurans and metabolite thereof and eight antibiotics, so that the specificity of the antibody is high. AMOZ of different amounts is added into four meat samples including fish, shrimp, chicken and pork liver, the ELISA is used for direct determination, the standard recovery rate ranges from 72.6% to 102.7%, the relative standard deviation is 6.1-17.7%, seven standard samples are detected by HPLC (high performance liquid chromatography) and ELISA simultaneously, determination results are compared, the regression curve is Y=0.9742X-7.6001, and the linearly dependent coefficient is 0.9889, so that the HPLC and the ELISA have high relativity.

Description

technical field [0001] The invention relates to an enzyme-linked immunosorbent assay method (ELISA) for directly detecting the content of furaltadone metabolite 5-morpholinemethyl-3-amino-2-oxazolidinone (AMOZ) in food, belonging to food safety supervision Or the research field of food analysis. Background technique [0002] Furaltadone (FTD) belongs to nitrofuran drugs and is a broad-spectrum antibiotic, which has been widely used in the prevention and treatment of dysentery, enteritis and coccidiosis in livestock and poultry. However, studies have shown that the drug has serious carcinogenic and other toxic and side effects. Therefore, countries around the world have banned the drug and limited the residue standards for more than ten years. However, there are still reports of furaltadone exceeding the standard at home and abroad from time to time. The furaltadone antibiotic is sensitive to light and has the characteristics of rapid metabolism. The half-life in animals is...

Claims

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Application Information

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IPC IPC(8): G01N33/64G01N33/531
Inventor 邓安平宋娟杨红
Owner SUZHOU UNIV
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