Newcastle disease virus rLX/H9HA and construction method and application thereof

Abstract

The invention discloses a recombination newcastle disease virus rLX/H9HA of H9 subtype avian influenza virus hemagglutinin albumen. A preservation number is CGMCCNo: 6652. According to the method, a reverse genetic operation platform of an established newcastle disease virus (DVA) weak poison LX strain is used, an HA gene of an H9N2 subtype avian influenza virus (AIV) epidemic strain A/Chicken/Jiangsu/WJ57/2012 is inserted in a genome overall length transcription carrier pLX of the LX strain, and thus a recombination newcastle disease virus genome overall length cDNA clone Plx-H9HA containing a H9N2 subtype AIVHA gene. A recombinant virus rLX/H9HA obtained through transfection on a chick embryo has high breeding geometric mean titer, can still express HA albumen stably through continuous passing of multiple generations, is suitable for mass production of vaccines, and can be used for vaccine manufacturing.

Description

technical field [0001] The invention relates to the application of reverse genetic technology to rescue a recombinant vaccine strain rLX / H9HA expressing the hemagglutinin protein of H9 subtype avian influenza virus with Newcastle disease virus as a carrier, and to use it for vaccine development. Background technique [0002] Reverse genetics manipulation technique (Reverse genetics manipulation technique) refers to the reverse transcription of RNA virus genomic RNA into cDNA, and the artificial construction of new infectious viruses in vitro by using methods such as gene mutation, gene insertion, gene knockout, and gene replacement. , to study the genome structure and function of the virus and the interaction between the virus host [Conzelmann KK. Also often referred to as "virus rescue". It solves the problem that RNA virus genome is difficult to operate, and at the same time provides a new idea for the development of new vaccines [Nakaya T, et al., Recombinant Newcastle d...

AI Technical Summary

Problems solved by technology

[0006] H9N2 subtype avian influenza virus is one of the important pathogens that endanger the current poultry industry. It exists widely in poultry. Although it is low pathogenicity, it has some unique pathogenic characteristics, mainly manifested as high infection rate and high incidence. rate, low mortality rate, often cause latent infection of chicks and rearing chickens and drop of egg production of laying hens, and the egg production rate is difficult to recover, which has caused serious harm to the poultry industry and has important public health significance

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