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Recombinant turkey herpesvirus strain rHMW for expressing H7N9 subtype avian influenza virus chimeric hemagglutinin and construction method of recombinant turkey herpesvirus strain rHMW

A technology of turkey herpes virus and avian influenza virus, which is applied in the field of recombinant virus vector vaccines, can solve the problems of decreased maternal antibody and early disease protection

Inactive Publication Date: 2017-03-15
YANGZHOU UNIV
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Problems solved by technology

However, when using MDV to construct a recombinant virus, the heterologous strong promoter is less regulated by the virus. In the early stage of recombinant virus immunity, the protective antigen gene controlled by the promoter is expressed in large quantities, which is different from the maternal source of the antigen in the body. An antibody reaction that reduces maternal antibodies against that antigen, reducing the body's early protection against a disease

Method used

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  • Recombinant turkey herpesvirus strain rHMW for expressing H7N9 subtype avian influenza virus chimeric hemagglutinin and construction method of recombinant turkey herpesvirus strain rHMW
  • Recombinant turkey herpesvirus strain rHMW for expressing H7N9 subtype avian influenza virus chimeric hemagglutinin and construction method of recombinant turkey herpesvirus strain rHMW
  • Recombinant turkey herpesvirus strain rHMW for expressing H7N9 subtype avian influenza virus chimeric hemagglutinin and construction method of recombinant turkey herpesvirus strain rHMW

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Embodiment Construction

[0029] Material

[0030] rHVT-GFP and pCMGFP were constructed and preserved by the Key Laboratory of Livestock and Poultry Infectious Diseases, Ministry of Agriculture, Yangzhou University (see patent application publication number: CN105002146A). H7N9 subtype avian influenza JX148 (A / chicken / Zhejiang / JX148 / 2014) (GISAID: EPI_ISL_235293), isolated and preserved by the Key Laboratory of Livestock and Poultry Infectious Diseases, Ministry of Agriculture, Yangzhou University, chicken embryo titer 10, hemagglutination inhibition reaction proof The polyantiserum against JX148 has high cross-reactivity with other subtypes of H7N9.

[0031] High fidelity DNA polymerase, T4 DNA ligase, and Agarose Gel DNA Extraction Kit were purchased from Roche Company; the plasmid extraction kit (QIAprep Spin MiniPrep Kit) was a product of QIAGEN Company; the rest of the conventional reagents were domestic analytical grade.

[0032] Step 1. Codon optimization and synthesis

[0033] According to th...

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Abstract

The invention belongs to recombinant viral vector vaccines in the technical fields of molecular biology and biology, and in particular relates to a recombinant turkey herpesvirus strain rHMW for expressing H7N9 subtype avian influenza virus chimeric hemagglutinin and a construction method of the recombinant turkey herpesvirus strain rHMW. The recombinant turkey herpesvirus strain rHMW has a collection number of CGMCC NO. 12984. The recombinant virus can be used for preparing vaccines capable of preventing H7N9 subtype avian influenza viruses. The recombinant turkey herpesvirus strain rHMW disclosed by the invention has obvious effects for preventing and controlling infectious diseases of poultry and achieves huge social benefits and economic benefits.

Description

technical field [0001] The invention belongs to the recombinant virus vector vaccine in the field of molecular biology and biotechnology, in particular to a recombinant turkey herpes virus rHMW capable of expressing the chimeric hemagglutinin protein of H7N9 subtype avian influenza virus, which can be used for immunization Use of poultry in preventing H7N9 subtype avian influenza virus. Background technique [0002] The H7N9 subtype avian influenza that infects humans first broke out in the Yangtze River Delta region of East my country in 2013. It is a new type of avian influenza virus that has been recombined from three sources and is non-pathogenic or low-pathogenic to poultry. So far, it has spread to all parts of the country except Tibet and the Inner Mongolia Autonomous Region. The Pearl River Delta is another new source of outbreaks after the Yangtze River Delta. According to WHO statistics, as of June 13, 2016, there were 781 cases of human infection with H7N9, of wh...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N7/01C12N15/869A61K39/145A61P31/16C12R1/93
CPCC07K14/005A61K39/00C12N15/86C12N2710/16021C12N2710/16043C12N2760/16122C12N2760/16134
Inventor 刘秀梵何东昌郝小利顾敏胡娇王晓泉刘晓文胡顺林
Owner YANGZHOU UNIV
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