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MRNA-based combined vaccine against coronavirus and influenza virus and preparation method of mRNA-based combined vaccine

A combined vaccine and influenza virus technology, applied in the field of medicine, can solve problems such as increasing the pain of patients, and achieve the effects of alleviating the pain of patients, enhancing humoral and cellular immune responses, and simple process

Pending Publication Date: 2021-03-26
IMMORNA (HANGZHOU) BIOTECHNOLOGY CO LTD
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0007] So far, there is no vaccine that can express the antigens of coronavirus and influenza virus at the same time in the market. It needs to be injected twice for different viruses, which increases the suffering of patients.

Method used

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  • MRNA-based combined vaccine against coronavirus and influenza virus and preparation method of mRNA-based combined vaccine
  • MRNA-based combined vaccine against coronavirus and influenza virus and preparation method of mRNA-based combined vaccine
  • MRNA-based combined vaccine against coronavirus and influenza virus and preparation method of mRNA-based combined vaccine

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preparation example Construction

[0063]Methods of preparation of combined vaccines for coronavirus and influenza viruses based on mRNA, including the following steps:

[0064]Step 1, the steps for preparing mRNA-Spike include:

[0065]Step A. Synthesate the SPIKE gene fragment;

[0066]Query the spike amino acid sequence, then optimize the optimized sequence according to the human predecessored codon table, and then add the promoter and APAI sink position (GGGCCC), downstream to the NOTICCCGC, The designed sequence is directly obtained by synthesis, and the gene sequence is shown in Seq ID NO.1 in the sequence list;

[0067]Step B, build TC-83 self-copy carrier;

[0068]Self-replicating mRNA is designed according to the genome of Venezuelama, Venezuea, Veezuequine Encephalitis (Veev) in the alpha virus family, which contains genes encoding alpha virus self-replication assemblies, but lacks coding for manufacturing The structural protein of sexual alpha viral particles, and the constructed plasmid is used as a template to amplify,...

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Abstract

The invention discloses an mRNA-based combined vaccine against coronavirus and influenza virus and a preparation method of the mRNA-based combined vaccine. The mRNA-based combined vaccine comprises aliposome-coated mRNA-Spike and liposome-coated mRNA-HA mixed combined vaccine and a liposome-coated mRNA-RBD-HA combined vaccine. mRNA is designed according to the genome of the venezuelan equine encephalomyelitis virus TC83 in the alpha virus family. The mRNA-Spike contains a Spike protein gene of the novel coronavirus pneumonia, the mRNA-HA contains a hemagglutinin protein gene of the influenza,and the mRNA-Spike contains a Spike protein gene of the nnoval coronavirus pneumonia and a hemagglutinin protein gene of the influenza. By using one mRNA, the antigen of the coronavirus and the antigen of the influenza virus can be expressed at the same time, high immunogenicity is achieved, the pain of a patient can be relieved, and the immune effect of injection of a single virus vaccine can beachieved.

Description

Technical field[0001]The present invention relates to a pharmaceutical field, particularly a combined vaccine for coronaviruses and influenza viruses based on mRNA.Background technique[0002]Coronavirus is a large viral family that can cause serious diseases such as ordinary colds and even Middle Eastern Respiratory Syndrome (MERS), severe acute respiratory syndrome (SARS), COVID19 and other serious diseases. The common signs of infection have respiratory symptoms, fever, cough, shortness of breath, and difficulty breathing. In a more serious case, infection can lead to pneumonia, severe acute respiratory syndrome, renal failure, and even death. Coronavirus is a virus that spreads between animals and humans. The detailed survey results show that severe acute respiratory syndrome is passed from the fruit raccoon to people, the Middle East respiratory syndrome is born from single peak camels. In addition, there are some known coronaviruses to spread in animals, but have not been infect...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K39/215A61K39/145A61P31/14A61P11/00A61P31/16C12N15/85C12N15/50C12N15/44
CPCA61K39/12A61K2039/54A61K2039/545A61K2039/57A61K2039/575A61K2039/70A61P11/00A61P31/14A61P31/16C07K14/005C12N15/85C12N2760/16122C12N2760/16134C12N2770/20022C12N2770/20034C12N2800/22
Inventor 王子豪
Owner IMMORNA (HANGZHOU) BIOTECHNOLOGY CO LTD
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