37 results about "Sprivivirus" patented technology

The invention provides fusion protein 210 and applications thereof in virus replication optimization. The fusion protein 210 is a truncated part of P60 protein, preserves a function for promoting replication of a plurality of viruses of the P60, and does not generate toxicity on cells in an effective concentration range. The fusion protein 210 can increase the virus titer by two orders of magnitudes in a wide-spectrum range (0.0001-0.1 MOI), and therefore virus identification and detection can be facilitated, rhabdovirus VSV, flaviviridae HCV, parvoviridae EV71, and the like can be detected, and time for detection is shortened by 50% or less. The fusion protein 210 obtained by a prokaryotic expression system is high in expression efficiency and easy to purify, the one-step purification efficiency can be 85% or above, and the protein final concentration can be 3 mg / mL. Accordingly, through directly adding the fusion protein 210 into a virus culture medium, a plurality of virus isolate strains can be detected under low titer conditions, and operation is simple, convenient and rapid. The fusion protein 210 has important influences and significance in the fields of production, learning, researching, and the like of the virology.

The invention discloses the application of compound PS-341 in the preparation of Picornaviridae Enterovirus inhibitors. The compound PS-341 has a structure shown in formula (I): the Picornaviridae Enterovirus virus For EV D68 virus, CV A16 virus. Experiments have proved that the compound PS‑341 inhibits the replication function of enteroviruses EV D68 and CV A16, and provides an effective solution for the prevention and treatment of enteroviruses EV D68 and CV A16.