Pelodiscus sinensis picornavirus complete genome sequence and applications thereof

A technology of RNA virus and Chinese soft-shelled turtle, which is applied to the genome sequence of Chinese soft-shelled turtle picorna virus and its application field, can solve the problems of lack of and unfavorable nucleic acid detection of Chinese soft-shelled turtle liver necrosis and enterohemorrhagic disease virus and the development of virus vaccines

Active Publication Date: 2013-11-13
ZHEJIANG INST OF FRESH WATER FISHERIES
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Problems solved by technology

[0004] In view of the lack of genome sequence information of Chinese soft-shelled turtle picornavirus in the prior art, which is not conducive to the detection of Chinese soft-shelled turtle liver necrosis enterohemorrhagic virus nucleic acid and the development of virus vaccines, the purpose of the present invention is to ...

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  • Pelodiscus sinensis picornavirus complete genome sequence and applications thereof
  • Pelodiscus sinensis picornavirus complete genome sequence and applications thereof
  • Pelodiscus sinensis picornavirus complete genome sequence and applications thereof

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Embodiment Construction

[0016] After extensive and in-depth research, the present inventors obtained for the first time the whole genome sequence of the Chinese soft-shelled turtle picornavirus with the characteristic of high-efficiency and stable proliferation on the spleen cells of Chinese soft-shelled turtle. On this basis, the present invention has been accomplished.

[0017] A polynucleotide of the invention may be in the form of DNA or RNA. Forms of DNA include cDNA, genomic DNA, or synthetic DNA. DNA can be single-stranded or double-stranded. DNA can be either the coding strand or the non-coding strand.

[0018] The complete genome sequence or its fragments of the present invention can usually be obtained by PCR amplification, recombination or artificial synthesis. For the PCR amplification method, primers can be designed according to the relevant nucleotide sequences disclosed in the present invention, especially the open reading frame sequence, and the cDNA library prepared by conventiona...

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Abstract

The invention relates to a separated nucleic acid molecule having a nucleotide sequence shown as SEQ ID NO:1 or an antisense sequence of the SEQ ID NO:1. The nucleic acid molecule is derived from pelodiscus sinensis picornavirus strain CNSR2011. The nucleic acid molecule can be used for preparing primers, probes or kits, wherein the primers, the probes and the kits are used for detecting the pelodiscus sinensis picornavirus. The nucleic acid molecule can also be used for preparing a vaccine of the pelodiscus sinensis picornavirus, and an anti-pelodiscus sinensis picornavirus antibody.

Description

technical field [0001] The present invention relates to the field of viral genomics. More specifically, the present invention relates to the genome sequence of Chinese soft-shelled turtle picornavirus and its application. Background technique [0002] The Chinese softshell turtle, Latin: Pelodiscus sinensis (Wiegmann, 1835), commonly known as softshell turtle, water fish, tuanyu and bastard in Chinese, and English common name: Chinese softshell turtle, belongs to the genus of the turtle family Turtle family of the turtle order, and is a precious and economical softshell turtle. Highly valuable aquatic animals. The soft-shelled soft-shelled turtle has delicious meat, rich nutrition and high protein content, especially the skirt is a popular delicacy. In 2010, the aquaculture output of soft-shelled soft-shelled turtle in China was 266,000 tons, of which the aquaculture output in Zhejiang Province accounted for 46.6% of the national soft-shelled soft-shelled turtle production...

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Application Information

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IPC IPC(8): C12N15/41C12Q1/70C12Q1/68C12N15/11A61K39/125A61P31/14C07K16/10C12R1/93
Inventor 潘晓艺沈锦玉蔺凌云姚嘉赟徐洋尹文林
Owner ZHEJIANG INST OF FRESH WATER FISHERIES
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