Human sepsis pathogen detection kit and detection method

Abstract

The invention provides a human sepsis pathogen detection kit and a corresponding detection method. The kit provided by the invention comprises primers and fluorescent probes fordetecting acinetobacter baumannii, klebsiella pneumoniae, streptococcus pneumoniae, bacteroides fragilis, staphylococcus aureus, pseudomonas aeruginosa, aeromonas hydrophila, escherichia coli, quail chicken enterococcus, enterobacter cloacae, stenotrophomonas maltophilia, coagulase negative staphylococcus, candida albicans, candida tropicalis, candida glabrata, cytomegalovirus, human herpesvirus type 4, human parvovirus B19, Candida parapsilosis, streptococcus.

Description

technical field [0001] The invention belongs to the technical field of molecular biology detection, in particular, the invention relates to a detection kit and a corresponding detection method for human sepsis pathogens. Background technique [0002] Sepsis is a syndrome in which infection causes dysregulation of the host response, leading to life-threatening organ dysfunction, and is a clinical syndrome with a high mortality rate. Sepsis not only seriously threatens human health, but also brings a huge economic burden to medical care. The fatality rate of sepsis in my country is higher than that in developed countries. A population-based epidemiological survey of sepsis found that 1,025,997 patients died of sepsis nationwide in 2015, accounting for 12.6% of the total case fatality rate. Another single-center retrospective study of 759 patients with severe sepsis from 2006 to 2016 showed that the mortality rate was as high as 36.6%. [0003] In May 2018, Chinese emergency...

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Problems solved by technology

At the same time, the positive rate of blood culture will be affected by factors such as blood collection volume, time of blood collection, frequency of blood collection, etc., which will interfere with the positive rate of blood culture in sepsis

In addition, it takes a long time to diagnose sepsis by blood culture, usually 48 to 72 hours

(2) Blood routine: It is currently considered clinically that white blood cells are 9 / L or greater than >20×10 9 / L should be highly suspected of infection, however, studies have shown that 50% of neonatal sepsis children's white blood cells can be within the normal range, and abnormal white blood cell counts can also appear in other pathological conditions, so the white blood cell count is very important for sepsis infection Diagnosis lacks sensitivity and specificity

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